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Alveolar macrophages develop from fetal monocytes that differentiate into long-lived cells in the first week of life via GM-CSF

(2013) JOURNAL OF EXPERIMENTAL MEDICINE. 210(10). p.1977-1992
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Abstract
Tissue-resident macrophages can develop from circulating adult monocytes or from primitive yolk sac-derived macrophages. The precise ontogeny of alveolar macrophages (AMFs) is unknown. By performing BrdU labeling and parabiosis experiments in adult mice, we found that circulating monocytes contributed minimally to the steady-state AMF pool. Mature AMFs were undetectable before birth and only fully colonized the alveolar space by 3 d after birth. Before birth, F4/80(hi)CD11b(lo) primitive macrophages and Ly6C(hi)CD11b(hi) fetal monocytes sequentially colonized the developing lung around E12.5 and E16.5, respectively. The first signs of AMF differentiation appeared around the saccular stage of lung development (E18.5). Adoptive transfer identified fetal monocytes, and not primitive macrophages, as the main precursors of AMFs. Fetal monocytes transferred to the lung of neonatal mice acquired an AMF phenotype via defined developmental stages over the course of one week, and persisted for at least three months. Early AMF commitment from fetal monocytes was absent in GM-CSF-deficient mice, whereas short-term perinatal intrapulmonary GM-CSF therapy rescued AMF development for weeks, although the resulting AMFs displayed an immature phenotype. This demonstrates that tissue-resident macrophages can also develop from fetal monocytes that adopt a stable phenotype shortly after birth in response to instructive cytokines, and then self-maintain throughout life.
Keywords
LANGERHANS CELLS, DEFICIENT MICE, DENDRITIC CELLS, TISSUE MACROPHAGES, LUNG MACROPHAGES, BLOOD MONOCYTES, LYMPHOID ORGANS, KUPFFER CELLS, SELF-RENEWAL, BONE-MARROW

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MLA
Guilliams, Martin, Ismé De Kleer, Sandrine Henri, et al. “Alveolar Macrophages Develop from Fetal Monocytes That Differentiate into Long-lived Cells in the First Week of Life via GM-CSF.” JOURNAL OF EXPERIMENTAL MEDICINE 210.10 (2013): 1977–1992. Print.
APA
Guilliams, M., De Kleer, I., Henri, S., Post, S., Vanhoutte, L., De Prijck, S., Deswarte, K., et al. (2013). Alveolar macrophages develop from fetal monocytes that differentiate into long-lived cells in the first week of life via GM-CSF. JOURNAL OF EXPERIMENTAL MEDICINE, 210(10), 1977–1992.
Chicago author-date
Guilliams, Martin, Ismé De Kleer, Sandrine Henri, Sijranke Post, Leen Vanhoutte, Sofie De Prijck, Kim Deswarte, Bernard Malissen, Hamida Hammad, and Bart Lambrecht. 2013. “Alveolar Macrophages Develop from Fetal Monocytes That Differentiate into Long-lived Cells in the First Week of Life via GM-CSF.” Journal of Experimental Medicine 210 (10): 1977–1992.
Chicago author-date (all authors)
Guilliams, Martin, Ismé De Kleer, Sandrine Henri, Sijranke Post, Leen Vanhoutte, Sofie De Prijck, Kim Deswarte, Bernard Malissen, Hamida Hammad, and Bart Lambrecht. 2013. “Alveolar Macrophages Develop from Fetal Monocytes That Differentiate into Long-lived Cells in the First Week of Life via GM-CSF.” Journal of Experimental Medicine 210 (10): 1977–1992.
Vancouver
1.
Guilliams M, De Kleer I, Henri S, Post S, Vanhoutte L, De Prijck S, et al. Alveolar macrophages develop from fetal monocytes that differentiate into long-lived cells in the first week of life via GM-CSF. JOURNAL OF EXPERIMENTAL MEDICINE. 2013;210(10):1977–92.
IEEE
[1]
M. Guilliams et al., “Alveolar macrophages develop from fetal monocytes that differentiate into long-lived cells in the first week of life via GM-CSF,” JOURNAL OF EXPERIMENTAL MEDICINE, vol. 210, no. 10, pp. 1977–1992, 2013.
@article{4178862,
  abstract     = {{Tissue-resident macrophages can develop from circulating adult monocytes or from primitive yolk sac-derived macrophages. The precise ontogeny of alveolar macrophages (AMFs) is unknown. By performing BrdU labeling and parabiosis experiments in adult mice, we found that circulating monocytes contributed minimally to the steady-state AMF pool. Mature AMFs were undetectable before birth and only fully colonized the alveolar space by 3 d after birth. Before birth, F4/80(hi)CD11b(lo) primitive macrophages and Ly6C(hi)CD11b(hi) fetal monocytes sequentially colonized the developing lung around E12.5 and E16.5, respectively. The first signs of AMF differentiation appeared around the saccular stage of lung development (E18.5). Adoptive transfer identified fetal monocytes, and not primitive macrophages, as the main precursors of AMFs. Fetal monocytes transferred to the lung of neonatal mice acquired an AMF phenotype via defined developmental stages over the course of one week, and persisted for at least three months. Early AMF commitment from fetal monocytes was absent in GM-CSF-deficient mice, whereas short-term perinatal intrapulmonary GM-CSF therapy rescued AMF development for weeks, although the resulting AMFs displayed an immature phenotype. This demonstrates that tissue-resident macrophages can also develop from fetal monocytes that adopt a stable phenotype shortly after birth in response to instructive cytokines, and then self-maintain throughout life.}},
  author       = {{Guilliams, Martin and De Kleer, Ismé and Henri, Sandrine and Post, Sijranke and Vanhoutte, Leen and De Prijck, Sofie and Deswarte, Kim and Malissen, Bernard and Hammad, Hamida and Lambrecht, Bart}},
  issn         = {{0022-1007}},
  journal      = {{JOURNAL OF EXPERIMENTAL MEDICINE}},
  keywords     = {{LANGERHANS CELLS,DEFICIENT MICE,DENDRITIC CELLS,TISSUE MACROPHAGES,LUNG MACROPHAGES,BLOOD MONOCYTES,LYMPHOID ORGANS,KUPFFER CELLS,SELF-RENEWAL,BONE-MARROW}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{1977--1992}},
  title        = {{Alveolar macrophages develop from fetal monocytes that differentiate into long-lived cells in the first week of life via GM-CSF}},
  url          = {{http://dx.doi.org/10.1084/jem.20131199}},
  volume       = {{210}},
  year         = {{2013}},
}

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