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Cloning and characterization of the NADPH cytochrome P450 reductase gene (CPR) from Candida bombicola

(2007) FEMS YEAST RESEARCH. 7(6). p.922-928
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Abstract
Candida bombicola is a yeast with at least two appealing features. The species can grow on alkanes when provided as the sole carbon source, and it produces glycolipids, which have several industrial, cosmetic and pharmaceutical applications. Both metabolic processes require in their pathway the activity of cytochrome P450 monooxygenase. This enzyme needs and gets reducing equivalents from NADPH cytochrome P450 reductase (CPR). The CPR gene of Candida bombicola was isolated using degenerate PCR and genomic walking. The gene encodes an enzyme of 687 amino acids, which shows homology with known CPRs of other species. The functionality of the gene was proven by heterologous expression in Escherichia coli. The recombinant protein exhibited NADPH-dependent cytochrome c reducing activity. Cloning and characterization of this enzyme is an important step in the study of the cytochrome P450 monooxygenase system of Candida bombicola. The GenBank accession number of the sequence described in this article is EF050789.
Keywords
ENDOPLASMIC-RETICULUM, TROPICALIS, OXIDOREDUCTASE GENE, MEMBRANE TOPOLOGY, MESSENGER-RNA, SYSTEM, SEQUENCES, MALTOSA, P-450 REDUCTASE, HETEROLOGOUS EXPRESSION, COX, Candida bombicola, NADPH cytochrome P450 reductase, phylogenetic tree, CPR, cytochrome c oxidase assembly protein

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Citation

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MLA
Van Bogaert, Inge, Dirk Develter, Wim Soetaert, et al. “Cloning and Characterization of the NADPH Cytochrome P450 Reductase Gene (CPR) from Candida Bombicola.” FEMS YEAST RESEARCH 7.6 (2007): 922–928. Print.
APA
Van Bogaert, Inge, Develter, D., Soetaert, W., & Vandamme, E. (2007). Cloning and characterization of the NADPH cytochrome P450 reductase gene (CPR) from Candida bombicola. FEMS YEAST RESEARCH, 7(6), 922–928.
Chicago author-date
Van Bogaert, Inge, Dirk Develter, Wim Soetaert, and Erick Vandamme. 2007. “Cloning and Characterization of the NADPH Cytochrome P450 Reductase Gene (CPR) from Candida Bombicola.” Fems Yeast Research 7 (6): 922–928.
Chicago author-date (all authors)
Van Bogaert, Inge, Dirk Develter, Wim Soetaert, and Erick Vandamme. 2007. “Cloning and Characterization of the NADPH Cytochrome P450 Reductase Gene (CPR) from Candida Bombicola.” Fems Yeast Research 7 (6): 922–928.
Vancouver
1.
Van Bogaert I, Develter D, Soetaert W, Vandamme E. Cloning and characterization of the NADPH cytochrome P450 reductase gene (CPR) from Candida bombicola. FEMS YEAST RESEARCH. 2007;7(6):922–8.
IEEE
[1]
I. Van Bogaert, D. Develter, W. Soetaert, and E. Vandamme, “Cloning and characterization of the NADPH cytochrome P450 reductase gene (CPR) from Candida bombicola,” FEMS YEAST RESEARCH, vol. 7, no. 6, pp. 922–928, 2007.
@article{417673,
  abstract     = {Candida bombicola is a yeast with at least two appealing features. The species can grow on alkanes when provided as the sole carbon source, and it produces glycolipids, which have several industrial, cosmetic and pharmaceutical applications. Both metabolic processes require in their pathway the activity of cytochrome P450 monooxygenase. This enzyme needs and gets reducing equivalents from NADPH cytochrome P450 reductase (CPR). The CPR gene of Candida bombicola was isolated using degenerate PCR and genomic walking. The gene encodes an enzyme of 687 amino acids, which shows homology with known CPRs of other species. The functionality of the gene was proven by heterologous expression in Escherichia coli. The recombinant protein exhibited NADPH-dependent cytochrome c reducing activity. Cloning and characterization of this enzyme is an important step in the study of the cytochrome P450 monooxygenase system of Candida bombicola. The GenBank accession number of the sequence described in this article is EF050789.},
  author       = {Van Bogaert, Inge and Develter, Dirk and Soetaert, Wim and Vandamme, Erick},
  issn         = {1567-1356},
  journal      = {FEMS YEAST RESEARCH},
  keywords     = {ENDOPLASMIC-RETICULUM,TROPICALIS,OXIDOREDUCTASE GENE,MEMBRANE TOPOLOGY,MESSENGER-RNA,SYSTEM,SEQUENCES,MALTOSA,P-450 REDUCTASE,HETEROLOGOUS EXPRESSION,COX,Candida bombicola,NADPH cytochrome P450 reductase,phylogenetic tree,CPR,cytochrome c oxidase assembly protein},
  language     = {eng},
  number       = {6},
  pages        = {922--928},
  title        = {Cloning and characterization of the NADPH cytochrome P450 reductase gene (CPR) from Candida bombicola},
  url          = {http://dx.doi.org/10.1111/j.1567-1364.2007.00262.x},
  volume       = {7},
  year         = {2007},
}

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