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Absence of Siglec-H in MCMV infection elevates interferon alpha production but does not enhance viral clearance

(2013) PLOS PATHOGENS. 9(9).
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Abstract
Plasmacytoid dendritic cells (pDCs) express the I-type lectin receptor Siglec-H and produce interferon alpha (IFN alpha), a critical antiviral cytokine during the acute phase of murine cytomegalovirus (MCMV) infection. The ligands and biological functions of Siglec-H still remain incompletely defined in vivo. Thus, we generated a novel bacterial artificial chromosome (BAC)-transgenic "pDCre" mouse which expresses Cre recombinase under the control of the Siglec-H promoter. By crossing these mice with a Rosa26 reporter strain, a representative fraction of Siglec-H+ pDCs is terminally labeled with red fluorescent protein (RFP). Interestingly, systemic MCMV infection of these mice causes the downregulation of Siglec-H surface expression. This decline occurs in a TLR9- and MyD88-dependent manner. To elucidate the functional role of Siglec-H during MCMV infection, we utilized a novel Siglec-H deficient mouse strain. In the absence of Siglec-H, the low infection rate of pDCs with MCMV remained unchanged, and pDC activation was still intact. Strikingly, Siglec-H deficiency induced a significant increase in serum IFNa levels following systemic MCMV infection. Although Siglec-H modulates anti-viral IFNa production, the control of viral replication was unchanged in vivo. The novel mouse models will be valuable to shed further light on pDC biology in future studies.
Keywords
ACTIVATION, PROGENITORS, I INTERFERON, MOUSE BONE-MARROW, BAC TRANSGENIC MICE, PERSISTENT LCMV INFECTION, PLASMACYTOID DENDRITIC CELLS, INNATE, TOLERANCE, CYTOMEGALOVIRUS-INFECTION

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Chicago
Puttur, Franz, Catharina Arnold-Schrauf, Katharina Lahl, Gulhas Solmaz, Marc Lindenberg, Christian Thomas Mayer, Melanie Gohmert, et al. 2013. “Absence of Siglec-H in MCMV Infection Elevates Interferon Alpha Production but Does Not Enhance Viral Clearance.” Plos Pathogens 9 (9).
APA
Puttur, F., Arnold-Schrauf, C., Lahl, K., Solmaz, G., Lindenberg, M., Mayer, C. T., Gohmert, M., et al. (2013). Absence of Siglec-H in MCMV infection elevates interferon alpha production but does not enhance viral clearance. PLOS PATHOGENS, 9(9).
Vancouver
1.
Puttur F, Arnold-Schrauf C, Lahl K, Solmaz G, Lindenberg M, Mayer CT, et al. Absence of Siglec-H in MCMV infection elevates interferon alpha production but does not enhance viral clearance. PLOS PATHOGENS. 2013;9(9).
MLA
Puttur, Franz, Catharina Arnold-Schrauf, Katharina Lahl, et al. “Absence of Siglec-H in MCMV Infection Elevates Interferon Alpha Production but Does Not Enhance Viral Clearance.” PLOS PATHOGENS 9.9 (2013): n. pag. Print.
@article{4172991,
  abstract     = {Plasmacytoid dendritic cells (pDCs) express the I-type lectin receptor Siglec-H and produce interferon alpha (IFN alpha), a critical antiviral cytokine during the acute phase of murine cytomegalovirus (MCMV) infection. The ligands and biological functions of Siglec-H still remain incompletely defined in vivo. Thus, we generated a novel bacterial artificial chromosome (BAC)-transgenic {\textacutedbl}pDCre{\textacutedbl} mouse which expresses Cre recombinase under the control of the Siglec-H promoter. By crossing these mice with a Rosa26 reporter strain, a representative fraction of Siglec-H+ pDCs is terminally labeled with red fluorescent protein (RFP). Interestingly, systemic MCMV infection of these mice causes the downregulation of Siglec-H surface expression. This decline occurs in a TLR9- and MyD88-dependent manner. To elucidate the functional role of Siglec-H during MCMV infection, we utilized a novel Siglec-H deficient mouse strain. In the absence of Siglec-H, the low infection rate of pDCs with MCMV remained unchanged, and pDC activation was still intact. Strikingly, Siglec-H deficiency induced a significant increase in serum IFNa levels following systemic MCMV infection. Although Siglec-H modulates anti-viral IFNa production, the control of viral replication was unchanged in vivo. The novel mouse models will be valuable to shed further light on pDC biology in future studies.},
  articleno    = {e1003648},
  author       = {Puttur, Franz and Arnold-Schrauf, Catharina and Lahl, Katharina and Solmaz, Gulhas and Lindenberg, Marc and Mayer, Christian Thomas and Gohmert, Melanie and Swallow, Maxine and van Helt, Christopher and Schmitt, Heike and Nitschke, Lars and Lambrecht, Bart and Lang, Roland and Messerle, Martin and Sparwasser, Tim},
  issn         = {1553-7374},
  journal      = {PLOS PATHOGENS},
  keyword      = {ACTIVATION,PROGENITORS,I INTERFERON,MOUSE BONE-MARROW,BAC TRANSGENIC MICE,PERSISTENT LCMV INFECTION,PLASMACYTOID DENDRITIC CELLS,INNATE,TOLERANCE,CYTOMEGALOVIRUS-INFECTION},
  language     = {eng},
  number       = {9},
  pages        = {12},
  title        = {Absence of Siglec-H in MCMV infection elevates interferon alpha production but does not enhance viral clearance},
  url          = {http://dx.doi.org/10.1371/journal.ppat.1003648},
  volume       = {9},
  year         = {2013},
}

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