Ghent University Academic Bibliography

Advanced

Enhancing pterin and para-aminobenzoate content is not sufficient to successfully biofortify potato tubers and Arabidopsis thaliana plants with folate

Dieter Blancquaert, Serguei Storojenko UGent, Jeroen Van Daele, Christophe Stove UGent, Richard GF Visser, Willy Lambert and Dominique Van Der Straeten UGent (2013) JOURNAL OF EXPERIMENTAL BOTANY. 64(12). p.3899-3909
abstract
Folates are important cofactors in one-carbon metabolism in all living organisms. Since only plants and micro- organisms are capable of biosynthesizing folates, humans depend entirely on their diet as a folate source. Given the low folate content of several staple crop products, folate deficiency affects regions all over the world. Folate biofortification of staple crops through enhancement of pterin and para-aminobenzoate levels, precursors of the folate biosynthesis pathway, was reported to be successful in tomato and rice. This study shows that the same strategy is not sufficient to enhance folate content in potato tubers and Arabidopsis thaliana plants and concludes that other steps in folate biosynthesis and/or metabolism need to be engineered to result in substantial folate accumulation. The findings provide a plausible explanation why, more than half a decade after the proof of concept in rice and tomato, successful folate biofortification of other food crops through enhancement of para-aminobenzoate and pterin content has not been reported thus far. A better understanding of the folate pathway is required in order to determine an engineering strategy that can be generalized to most staple crops.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
TIME QUANTITATIVE PCR, tuber, FOLIC-ACID, TOMATO FRUIT, GENE, EXPRESSION, TRANSFORMATION, HOMOCYSTEINE, PROMOTER, DISEASE, FORTIFICATION, potato, metabolic engineering, folic acid, folate, biofortification, Arabidopsis thaliana, para-aminobenzoate, pterin
journal title
JOURNAL OF EXPERIMENTAL BOTANY
J. Exp. Bot.
volume
64
issue
12
pages
3899 - 3909
Web of Science type
Article
Web of Science id
000323578700028
JCR category
PLANT SCIENCES
JCR impact factor
5.794 (2013)
JCR rank
11/199 (2013)
JCR quartile
1 (2013)
ISSN
0022-0957
DOI
10.1093/jxb/ert224
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4168547
handle
http://hdl.handle.net/1854/LU-4168547
date created
2013-10-22 11:07:57
date last changed
2016-12-19 15:39:44
@article{4168547,
  abstract     = {Folates are important cofactors in one-carbon metabolism in all living organisms. Since only plants and micro- organisms are capable of biosynthesizing folates, humans depend entirely on their diet as a folate source. Given the low folate content of several staple crop products, folate deficiency affects regions all over the world. Folate biofortification of staple crops through enhancement of pterin and para-aminobenzoate levels, precursors of the folate biosynthesis pathway, was reported to be successful in tomato and rice. This study shows that the same strategy is not sufficient to enhance folate content in potato tubers and Arabidopsis thaliana plants and concludes that other steps in folate biosynthesis and/or metabolism need to be engineered to result in substantial folate accumulation. The findings provide a plausible explanation why, more than half a decade after the proof of concept in rice and tomato, successful folate biofortification of other food crops through enhancement of para-aminobenzoate and pterin content has not been reported thus far. A better understanding of the folate pathway is required in order to determine an engineering strategy that can be generalized to most staple crops.},
  author       = {Blancquaert, Dieter and Storojenko, Serguei and Van Daele, Jeroen and Stove, Christophe and Visser, Richard GF and Lambert, Willy and Van Der Straeten, Dominique},
  issn         = {0022-0957},
  journal      = {JOURNAL OF EXPERIMENTAL BOTANY},
  keyword      = {TIME QUANTITATIVE PCR,tuber,FOLIC-ACID,TOMATO FRUIT,GENE,EXPRESSION,TRANSFORMATION,HOMOCYSTEINE,PROMOTER,DISEASE,FORTIFICATION,potato,metabolic engineering,folic acid,folate,biofortification,Arabidopsis thaliana,para-aminobenzoate,pterin},
  language     = {eng},
  number       = {12},
  pages        = {3899--3909},
  title        = {Enhancing pterin and para-aminobenzoate content is not sufficient to successfully biofortify potato tubers and Arabidopsis thaliana plants with folate},
  url          = {http://dx.doi.org/10.1093/jxb/ert224},
  volume       = {64},
  year         = {2013},
}

Chicago
Blancquaert, Dieter, Serguei Storojenko, Jeroen Van Daele, Christophe Stove, Richard GF Visser, Willy Lambert, and Dominique Van Der Straeten. 2013. “Enhancing Pterin and Para-aminobenzoate Content Is Not Sufficient to Successfully Biofortify Potato Tubers and Arabidopsis Thaliana Plants with Folate.” Journal of Experimental Botany 64 (12): 3899–3909.
APA
Blancquaert, D., Storojenko, S., Van Daele, J., Stove, C., Visser, R. G., Lambert, W., & Van Der Straeten, D. (2013). Enhancing pterin and para-aminobenzoate content is not sufficient to successfully biofortify potato tubers and Arabidopsis thaliana plants with folate. JOURNAL OF EXPERIMENTAL BOTANY, 64(12), 3899–3909.
Vancouver
1.
Blancquaert D, Storojenko S, Van Daele J, Stove C, Visser RG, Lambert W, et al. Enhancing pterin and para-aminobenzoate content is not sufficient to successfully biofortify potato tubers and Arabidopsis thaliana plants with folate. JOURNAL OF EXPERIMENTAL BOTANY. 2013;64(12):3899–909.
MLA
Blancquaert, Dieter, Serguei Storojenko, Jeroen Van Daele, et al. “Enhancing Pterin and Para-aminobenzoate Content Is Not Sufficient to Successfully Biofortify Potato Tubers and Arabidopsis Thaliana Plants with Folate.” JOURNAL OF EXPERIMENTAL BOTANY 64.12 (2013): 3899–3909. Print.