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Identification of a microscopically selected microorganism in milk samples

(2014) JOURNAL OF DAIRY SCIENCE. 97(2). p.609-615
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Abstract
Identification of unwanted microbial contaminants microscopically observed in food products is challenging due to their low abundance in a complex matrix, quite often containing other microorganisms. Therefore, a selective identification method was developed using laser capture microdissection in combination with direct-captured cell PCR. This procedure was validated with Geobacillus stearothermophilus and further used to identify microbial contaminants present in some industrial milk samples. The microscopically observed contaminants were identified as mainly Methylobacterium species.
Keywords
Laser capture microdissection, cultivation independent identification, bacterial contamination, direct captured cell PCR, LASER-CAPTURE MICRODISSECTION, SEQUENCE-ANALYSIS, FOOD-INDUSTRY, FRESH-WATER, DNA, POPULATIONS, BACTERIA, CULTURE, PCR, INFECTION

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MLA
Bracke, Nathalie, Mario Van Poucke, Bram Baert, et al. “Identification of a Microscopically Selected Microorganism in Milk Samples.” JOURNAL OF DAIRY SCIENCE 97.2 (2014): 609–615. Print.
APA
Bracke, Nathalie, Van Poucke, M., Baert, B., Wynendaele, E., De Bels, L., Van Den Broeck, W., Peelman, L., et al. (2014). Identification of a microscopically selected microorganism in milk samples. JOURNAL OF DAIRY SCIENCE, 97(2), 609–615.
Chicago author-date
Bracke, Nathalie, Mario Van Poucke, Bram Baert, Evelien Wynendaele, Lobke De Bels, Wim Van Den Broeck, Luc Peelman, Christian Burvenich, and Bart De Spiegeleer. 2014. “Identification of a Microscopically Selected Microorganism in Milk Samples.” Journal of Dairy Science 97 (2): 609–615.
Chicago author-date (all authors)
Bracke, Nathalie, Mario Van Poucke, Bram Baert, Evelien Wynendaele, Lobke De Bels, Wim Van Den Broeck, Luc Peelman, Christian Burvenich, and Bart De Spiegeleer. 2014. “Identification of a Microscopically Selected Microorganism in Milk Samples.” Journal of Dairy Science 97 (2): 609–615.
Vancouver
1.
Bracke N, Van Poucke M, Baert B, Wynendaele E, De Bels L, Van Den Broeck W, et al. Identification of a microscopically selected microorganism in milk samples. JOURNAL OF DAIRY SCIENCE. 2014;97(2):609–15.
IEEE
[1]
N. Bracke et al., “Identification of a microscopically selected microorganism in milk samples,” JOURNAL OF DAIRY SCIENCE, vol. 97, no. 2, pp. 609–615, 2014.
@article{4163872,
  abstract     = {Identification of unwanted microbial contaminants microscopically observed in food products is challenging due to their low abundance in a complex matrix, quite often containing other microorganisms. Therefore, a selective identification method was developed using laser capture microdissection in combination with direct-captured cell PCR. This procedure was validated with Geobacillus stearothermophilus and further used to identify microbial contaminants present in some industrial milk samples. The microscopically observed contaminants were identified as mainly Methylobacterium species.},
  author       = {Bracke, Nathalie and Van Poucke, Mario and Baert, Bram and Wynendaele, Evelien and De Bels, Lobke and Van Den Broeck, Wim and Peelman, Luc and Burvenich, Christian and De Spiegeleer, Bart},
  issn         = {0022-0302},
  journal      = {JOURNAL OF DAIRY SCIENCE},
  keywords     = {Laser capture microdissection,cultivation independent identification,bacterial contamination,direct captured cell PCR,LASER-CAPTURE MICRODISSECTION,SEQUENCE-ANALYSIS,FOOD-INDUSTRY,FRESH-WATER,DNA,POPULATIONS,BACTERIA,CULTURE,PCR,INFECTION},
  language     = {eng},
  number       = {2},
  pages        = {609--615},
  title        = {Identification of a microscopically selected microorganism in milk samples},
  url          = {http://dx.doi.org/10.3168/jds.2013-6932},
  volume       = {97},
  year         = {2014},
}

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