Advanced search
1 file | 3.08 MB Add to list

Establishment of feline intestinal epithelial cell cultures for the propagation and study of feline enteric coronaviruses

Lowiese Desmarets (UGent) , Sebastiaan Theuns (UGent) , Dominique Olyslaegers (UGent) , Annelike Dedeurwaerder (UGent) , Ben Vermeulen (UGent) , Inge Roukaerts (UGent) and Hans Nauwynck (UGent)
Author
Organization
Abstract
Feline infectious peritonitis (FIP) is the most feared infectious cause of death in cats, induced by feline infectious peritonitis virus (FIPV). This coronavirus is a virulent mutant of the harmless, ubiquitous feline enteric coronavirus (FECV). To date, feline coronavirus (FCoV) research has been hampered by the lack of susceptible cell lines for the propagation of serotype I FCoVs. In this study, long-term feline intestinal epithelial cell cultures were established from primary ileocytes and colonocytes by simian virus 40 (SV40) T-antigen-and human Telomerase Reverse Transcriptase (hTERT)-induced immortalization. Subsequently, these cultures were evaluated for their usability in FCoV research. Firstly, the replication capacity of the serotype II strains WSU 79-1683 and WSU 79-1146 was studied in the continuous cultures as was done for the primary cultures. In accordance with the results obtained in primary cultures, FCoV WSU 79-1683 still replicated significantly more efficient compared to FCoV WSU 79-1146 in both continuous cultures. In addition, the cultures were inoculated with faecal suspensions from healthy cats and with faecal or tissue suspensions from FIP cats. The cultures were susceptible to infection with different serotype I enteric strains and two of these strains were further propagated. No infection was seen in cultures inoculated with FIPV tissue homogenates. In conclusion, a new reliable model for FCoV investigation and growth of enteric field strains was established. In contrast to FIPV strains, FECVs showed a clear tropism for intestinal epithelial cells, giving an explanation for the observation that FECV is the main pathotype circulating among cats.
Keywords
REPLICATION, PATHOGENICITY, PREVALENCE, EVOLUTION, HEALTHY CATS, 3C GENE, PATHOGENESIS, PERSISTENCE, INFECTIOUS PERITONITIS, SPIKE PROTEIN

Downloads

  • 1297-9716-44-71.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 3.08 MB

Citation

Please use this url to cite or link to this publication:

MLA
Desmarets, Lowiese et al. “Establishment of Feline Intestinal Epithelial Cell Cultures for the Propagation and Study of Feline Enteric Coronaviruses.” VETERINARY RESEARCH 44 (2013): n. pag. Print.
APA
Desmarets, L., Theuns, S., Olyslaegers, D., Dedeurwaerder, A., Vermeulen, B., Roukaerts, I., & Nauwynck, H. (2013). Establishment of feline intestinal epithelial cell cultures for the propagation and study of feline enteric coronaviruses. VETERINARY RESEARCH, 44.
Chicago author-date
Desmarets, Lowiese, Sebastiaan Theuns, Dominique Olyslaegers, Annelike Dedeurwaerder, Ben Vermeulen, Inge Roukaerts, and Hans Nauwynck. 2013. “Establishment of Feline Intestinal Epithelial Cell Cultures for the Propagation and Study of Feline Enteric Coronaviruses.” Veterinary Research 44.
Chicago author-date (all authors)
Desmarets, Lowiese, Sebastiaan Theuns, Dominique Olyslaegers, Annelike Dedeurwaerder, Ben Vermeulen, Inge Roukaerts, and Hans Nauwynck. 2013. “Establishment of Feline Intestinal Epithelial Cell Cultures for the Propagation and Study of Feline Enteric Coronaviruses.” Veterinary Research 44.
Vancouver
1.
Desmarets L, Theuns S, Olyslaegers D, Dedeurwaerder A, Vermeulen B, Roukaerts I, et al. Establishment of feline intestinal epithelial cell cultures for the propagation and study of feline enteric coronaviruses. VETERINARY RESEARCH. 2013;44.
IEEE
[1]
L. Desmarets et al., “Establishment of feline intestinal epithelial cell cultures for the propagation and study of feline enteric coronaviruses,” VETERINARY RESEARCH, vol. 44, 2013.
@article{4158054,
  abstract     = {Feline infectious peritonitis (FIP) is the most feared infectious cause of death in cats, induced by feline infectious peritonitis virus (FIPV). This coronavirus is a virulent mutant of the harmless, ubiquitous feline enteric coronavirus (FECV). To date, feline coronavirus (FCoV) research has been hampered by the lack of susceptible cell lines for the propagation of serotype I FCoVs. In this study, long-term feline intestinal epithelial cell cultures were established from primary ileocytes and colonocytes by simian virus 40 (SV40) T-antigen-and human Telomerase Reverse Transcriptase (hTERT)-induced immortalization. Subsequently, these cultures were evaluated for their usability in FCoV research. Firstly, the replication capacity of the serotype II strains WSU 79-1683 and WSU 79-1146 was studied in the continuous cultures as was done for the primary cultures. In accordance with the results obtained in primary cultures, FCoV WSU 79-1683 still replicated significantly more efficient compared to FCoV WSU 79-1146 in both continuous cultures. In addition, the cultures were inoculated with faecal suspensions from healthy cats and with faecal or tissue suspensions from FIP cats. The cultures were susceptible to infection with different serotype I enteric strains and two of these strains were further propagated. No infection was seen in cultures inoculated with FIPV tissue homogenates. In conclusion, a new reliable model for FCoV investigation and growth of enteric field strains was established. In contrast to FIPV strains, FECVs showed a clear tropism for intestinal epithelial cells, giving an explanation for the observation that FECV is the main pathotype circulating among cats.},
  articleno    = {71},
  author       = {Desmarets, Lowiese and Theuns, Sebastiaan and Olyslaegers, Dominique and Dedeurwaerder, Annelike and Vermeulen, Ben and Roukaerts, Inge and Nauwynck, Hans},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keywords     = {REPLICATION,PATHOGENICITY,PREVALENCE,EVOLUTION,HEALTHY CATS,3C GENE,PATHOGENESIS,PERSISTENCE,INFECTIOUS PERITONITIS,SPIKE PROTEIN},
  language     = {eng},
  pages        = {13},
  title        = {Establishment of feline intestinal epithelial cell cultures for the propagation and study of feline enteric coronaviruses},
  url          = {http://dx.doi.org/10.1186/1297-9716-44-71},
  volume       = {44},
  year         = {2013},
}

Altmetric
View in Altmetric
Web of Science
Times cited: