Advanced search
1 file | 506.51 KB

Differential response to acute low dose radiation in primary and immortalized endothelial cells

Author
Organization
Project
Center for nano- and biophotonics (NB-Photonics)
Abstract
Purpose : The low dose radiation response of primary human umbilical vein endothelial cells (HUVEC) and its immortalized derivative, the EA.hy926 cell line, was evaluated and compared. Material and methods: DNA damage and repair, cell cycle progression, apoptosis and cellular morphology in HUVEC and EA.hy926 were evaluated after exposure to low (0.05-0.5 Gy) and high doses (2 and 5 Gy) of acute X-rays. Results : Subtle, but significant increases in DNA double-strand breaks (DSB) were observed in HUVEC and EA.hy926 30 min after low dose irradiation (0.05 Gy). Compared to high dose irradiation (2 Gy), relatively more DSB/Gy were formed after low dose irradiation. Also, we observed a dose-dependent increase in apoptotic cells, down to 0.5 Gy in HUVEC and 0.1 Gy in EA.hy926 cells. Furthermore, radiation induced significantly more apoptosis in EA.hy926 compared to HUVEC. Conclusions : We demonstrated for the first time that acute low doses of X-rays induce DNA damage and apoptosis in endothelial cells. Our results point to a non-linear dose-response relationship for DSB formation in endothelial cells. Furthermore, the observed difference in radiation-induced apoptosis points to a higher radiosensitivity of EA.hy926 compared to HUVEC, which should be taken into account when using these cells as models for studying the endothelium radiation response.
Keywords
endothelial cells, Low dose X-radiation, DNA damage, cell cycle, apoptosis, DNA-DAMAGE, VASCULAR-DISEASE, X-IRRADIATION, APOPTOSIS, DEATH, MECHANISMS, ATHEROSCLEROSIS, DYSFUNCTION, STRESS, LINE

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 506.51 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Rombouts, Charlotte, An Aerts, Michaël Beck, Winnok De Vos, Patric Van Oostveldt, Mohammed Abderrafi Benotmane, and Sarah Baatout. 2013. “Differential Response to Acute Low Dose Radiation in Primary and Immortalized Endothelial Cells.” International Journal of Radiation Biology 89 (10): 841–850.
APA
Rombouts, Charlotte, Aerts, A., Beck, M., De Vos, W., Van Oostveldt, P., Benotmane, M. A., & Baatout, S. (2013). Differential response to acute low dose radiation in primary and immortalized endothelial cells. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 89(10), 841–850.
Vancouver
1.
Rombouts C, Aerts A, Beck M, De Vos W, Van Oostveldt P, Benotmane MA, et al. Differential response to acute low dose radiation in primary and immortalized endothelial cells. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY. 2013;89(10):841–50.
MLA
Rombouts, Charlotte, An Aerts, Michaël Beck, et al. “Differential Response to Acute Low Dose Radiation in Primary and Immortalized Endothelial Cells.” INTERNATIONAL JOURNAL OF RADIATION BIOLOGY 89.10 (2013): 841–850. Print.
@article{4157362,
  abstract     = {Purpose : The low dose radiation response of primary human umbilical vein endothelial cells (HUVEC) and its immortalized derivative, the EA.hy926 cell line, was evaluated and compared. 
Material and methods: DNA damage and repair, cell cycle progression, apoptosis and cellular morphology in HUVEC and EA.hy926 were evaluated after exposure to low (0.05-0.5 Gy) and high doses (2 and 5 Gy) of acute X-rays. 
Results : Subtle, but significant increases in DNA double-strand breaks (DSB) were observed in HUVEC and EA.hy926 30 min after low dose irradiation (0.05 Gy). Compared to high dose irradiation (2 Gy), relatively more DSB/Gy were formed after low dose irradiation. Also, we observed a dose-dependent increase in apoptotic cells, down to 0.5 Gy in HUVEC and 0.1 Gy in EA.hy926 cells. Furthermore, radiation induced significantly more apoptosis in EA.hy926 compared to HUVEC. 
Conclusions : We demonstrated for the first time that acute low doses of X-rays induce DNA damage and apoptosis in endothelial cells. Our results point to a non-linear dose-response relationship for DSB formation in endothelial cells. Furthermore, the observed difference in radiation-induced apoptosis points to a higher radiosensitivity of EA.hy926 compared to HUVEC, which should be taken into account when using these cells as models for studying the endothelium radiation response.},
  author       = {Rombouts, Charlotte and Aerts, An and Beck, Micha{\"e}l and De Vos, Winnok and Van Oostveldt, Patric and Benotmane, Mohammed Abderrafi and Baatout, Sarah},
  issn         = {0955-3002},
  journal      = {INTERNATIONAL JOURNAL OF RADIATION BIOLOGY},
  keyword      = {endothelial cells,Low dose X-radiation,DNA damage,cell cycle,apoptosis,DNA-DAMAGE,VASCULAR-DISEASE,X-IRRADIATION,APOPTOSIS,DEATH,MECHANISMS,ATHEROSCLEROSIS,DYSFUNCTION,STRESS,LINE},
  language     = {eng},
  number       = {10},
  pages        = {841--850},
  title        = {Differential response to acute low dose radiation in primary and immortalized endothelial cells},
  url          = {http://dx.doi.org/10.3109/09553002.2013.806831},
  volume       = {89},
  year         = {2013},
}

Altmetric
View in Altmetric
Web of Science
Times cited: