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Differential response to acute low dose radiation in primary and immortalized endothelial cells

Charlotte Rombouts, An Aerts, Michaël Beck, Winnok De Vos UGent, Patric Van Oostveldt UGent, Mohammed Abderrafi Benotmane and Sarah Baatout UGent (2013) INTERNATIONAL JOURNAL OF RADIATION BIOLOGY. 89(10). p.841-850
abstract
Purpose : The low dose radiation response of primary human umbilical vein endothelial cells (HUVEC) and its immortalized derivative, the EA.hy926 cell line, was evaluated and compared. Material and methods: DNA damage and repair, cell cycle progression, apoptosis and cellular morphology in HUVEC and EA.hy926 were evaluated after exposure to low (0.05-0.5 Gy) and high doses (2 and 5 Gy) of acute X-rays. Results : Subtle, but significant increases in DNA double-strand breaks (DSB) were observed in HUVEC and EA.hy926 30 min after low dose irradiation (0.05 Gy). Compared to high dose irradiation (2 Gy), relatively more DSB/Gy were formed after low dose irradiation. Also, we observed a dose-dependent increase in apoptotic cells, down to 0.5 Gy in HUVEC and 0.1 Gy in EA.hy926 cells. Furthermore, radiation induced significantly more apoptosis in EA.hy926 compared to HUVEC. Conclusions : We demonstrated for the first time that acute low doses of X-rays induce DNA damage and apoptosis in endothelial cells. Our results point to a non-linear dose-response relationship for DSB formation in endothelial cells. Furthermore, the observed difference in radiation-induced apoptosis points to a higher radiosensitivity of EA.hy926 compared to HUVEC, which should be taken into account when using these cells as models for studying the endothelium radiation response.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
endothelial cells, Low dose X-radiation, DNA damage, cell cycle, apoptosis, DNA-DAMAGE, VASCULAR-DISEASE, X-IRRADIATION, APOPTOSIS, DEATH, MECHANISMS, ATHEROSCLEROSIS, DYSFUNCTION, STRESS, LINE
journal title
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
Int. J. Radiat. Biol.
volume
89
issue
10
pages
841 - 850
Web of Science type
Article
Web of Science id
000325358500009
JCR category
NUCLEAR SCIENCE & TECHNOLOGY
JCR impact factor
1.837 (2013)
JCR rank
3/33 (2013)
JCR quartile
1 (2013)
ISSN
0955-3002
DOI
10.3109/09553002.2013.806831
project
Center for nano- and biophotonics (NB-Photonics)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4157362
handle
http://hdl.handle.net/1854/LU-4157362
date created
2013-10-07 17:08:01
date last changed
2016-12-19 15:44:33
@article{4157362,
  abstract     = {Purpose : The low dose radiation response of primary human umbilical vein endothelial cells (HUVEC) and its immortalized derivative, the EA.hy926 cell line, was evaluated and compared. 
Material and methods: DNA damage and repair, cell cycle progression, apoptosis and cellular morphology in HUVEC and EA.hy926 were evaluated after exposure to low (0.05-0.5 Gy) and high doses (2 and 5 Gy) of acute X-rays. 
Results : Subtle, but significant increases in DNA double-strand breaks (DSB) were observed in HUVEC and EA.hy926 30 min after low dose irradiation (0.05 Gy). Compared to high dose irradiation (2 Gy), relatively more DSB/Gy were formed after low dose irradiation. Also, we observed a dose-dependent increase in apoptotic cells, down to 0.5 Gy in HUVEC and 0.1 Gy in EA.hy926 cells. Furthermore, radiation induced significantly more apoptosis in EA.hy926 compared to HUVEC. 
Conclusions : We demonstrated for the first time that acute low doses of X-rays induce DNA damage and apoptosis in endothelial cells. Our results point to a non-linear dose-response relationship for DSB formation in endothelial cells. Furthermore, the observed difference in radiation-induced apoptosis points to a higher radiosensitivity of EA.hy926 compared to HUVEC, which should be taken into account when using these cells as models for studying the endothelium radiation response.},
  author       = {Rombouts, Charlotte and Aerts, An and Beck, Micha{\"e}l and De Vos, Winnok and Van Oostveldt, Patric and Benotmane, Mohammed Abderrafi and Baatout, Sarah},
  issn         = {0955-3002},
  journal      = {INTERNATIONAL JOURNAL OF RADIATION BIOLOGY},
  keyword      = {endothelial cells,Low dose X-radiation,DNA damage,cell cycle,apoptosis,DNA-DAMAGE,VASCULAR-DISEASE,X-IRRADIATION,APOPTOSIS,DEATH,MECHANISMS,ATHEROSCLEROSIS,DYSFUNCTION,STRESS,LINE},
  language     = {eng},
  number       = {10},
  pages        = {841--850},
  title        = {Differential response to acute low dose radiation in primary and immortalized endothelial cells},
  url          = {http://dx.doi.org/10.3109/09553002.2013.806831},
  volume       = {89},
  year         = {2013},
}

Chicago
Rombouts, Charlotte, An Aerts, Michaël Beck, Winnok De Vos, Patric Van Oostveldt, Mohammed Abderrafi Benotmane, and Sarah Baatout. 2013. “Differential Response to Acute Low Dose Radiation in Primary and Immortalized Endothelial Cells.” International Journal of Radiation Biology 89 (10): 841–850.
APA
Rombouts, Charlotte, Aerts, A., Beck, M., De Vos, W., Van Oostveldt, P., Benotmane, M. A., & Baatout, S. (2013). Differential response to acute low dose radiation in primary and immortalized endothelial cells. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 89(10), 841–850.
Vancouver
1.
Rombouts C, Aerts A, Beck M, De Vos W, Van Oostveldt P, Benotmane MA, et al. Differential response to acute low dose radiation in primary and immortalized endothelial cells. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY. 2013;89(10):841–50.
MLA
Rombouts, Charlotte, An Aerts, Michaël Beck, et al. “Differential Response to Acute Low Dose Radiation in Primary and Immortalized Endothelial Cells.” INTERNATIONAL JOURNAL OF RADIATION BIOLOGY 89.10 (2013): 841–850. Print.