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Reduced gibberellin response affects ethylene biosynthesis and responsiveness in the Arabidopsis gai eto2-1 double mutant

(2008) NEW PHYTOLOGIST. 177(1). p.128-141
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Abstract
Ethylene and gibberellins (GAs) control similar developmental processes in plants. The role of ethylene is at least in part to regulate the accumulation of DELLA proteins, key regulators of plant growth, which suppress the GA response. To expand our knowledge of ethylene-GA crosstalk and to reveal how the modulation of the ethylene and GA pathways affects global plant growth, the gibberellin-insensitive (gai), ethylene-overproducing 2-1 (eto2-1) double mutant, which has decreased GA signalling (resulting from gai) and increased ethylene biosynthesis (resulting from eto2-1), was characterized. Both single mutations resulted in reduced elongation growth. The double mutant showed synergistic responses in root and shoot growth, in induction of floral transition, and in inflorescence length, showing that crosstalk between the two pathways occurs in different plant organs throughout development. Furthermore, the altered ethylene-GA interactions affected root-shoot communication, as evidenced by an enhanced shoot:root ratio in the double mutant. When compared with both single mutants and the wild type, double mutants had enhanced content of active GA(4) at both the seedling and the rosette stages, and, unlike the gai mutant, they were sensitive to GA treatment. Finally, it was shown that synergistic responses in the double mutant were not caused by elevated ethylene biosynthesis but that, in the light, enhanced sensitivity to ethylene may, at least in part, be responsible for the observed phenotype.
Keywords
PROTEIN, ENCODES, PLANT-GROWTH, THALIANA, GENE-EXPRESSION, NEGATIVE REGULATOR, HYPOCOTYL ELONGATION, ROOT-GROWTH, SEED-GERMINATION, E3 UBIQUITIN LIGASE, root : shoot ratio, growth, gibberellins, ethylene, arabidopsis, crosstalk

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Citation

Please use this url to cite or link to this publication:

Chicago
De Grauwe, Liesbeth, Laury Chaerle, Jasper Dugardeyn, Jan Decat, Ivo Rieu, Willem Vriezen, Thomas Moritz, et al. 2008. “Reduced Gibberellin Response Affects Ethylene Biosynthesis and Responsiveness in the Arabidopsis Gai Eto2-1 Double Mutant.” New Phytologist 177 (1): 128–141.
APA
De Grauwe, Liesbeth, Chaerle, L., Dugardeyn, J., Decat, J., Rieu, I., Vriezen, W., Moritz, T., et al. (2008). Reduced gibberellin response affects ethylene biosynthesis and responsiveness in the Arabidopsis gai eto2-1 double mutant. NEW PHYTOLOGIST, 177(1), 128–141.
Vancouver
1.
De Grauwe L, Chaerle L, Dugardeyn J, Decat J, Rieu I, Vriezen W, et al. Reduced gibberellin response affects ethylene biosynthesis and responsiveness in the Arabidopsis gai eto2-1 double mutant. NEW PHYTOLOGIST. 2008;177(1):128–41.
MLA
De Grauwe, Liesbeth, Laury Chaerle, Jasper Dugardeyn, et al. “Reduced Gibberellin Response Affects Ethylene Biosynthesis and Responsiveness in the Arabidopsis Gai Eto2-1 Double Mutant.” NEW PHYTOLOGIST 177.1 (2008): 128–141. Print.
@article{414468,
  abstract     = {Ethylene and gibberellins (GAs) control similar developmental processes in plants. The role of ethylene is at least in part to regulate the accumulation of DELLA proteins, key regulators of plant growth, which suppress the GA response. 
To expand our knowledge of ethylene-GA crosstalk and to reveal how the modulation of the ethylene and GA pathways affects global plant growth, the gibberellin-insensitive (gai), ethylene-overproducing 2-1 (eto2-1) double mutant, which has decreased GA signalling (resulting from gai) and increased ethylene biosynthesis (resulting from eto2-1), was characterized. 
Both single mutations resulted in reduced elongation growth. The double mutant showed synergistic responses in root and shoot growth, in induction of floral transition, and in inflorescence length, showing that crosstalk between the two pathways occurs in different plant organs throughout development. Furthermore, the altered ethylene-GA interactions affected root-shoot communication, as evidenced by an enhanced shoot:root ratio in the double mutant. When compared with both single mutants and the wild type, double mutants had enhanced content of active GA(4) at both the seedling and the rosette stages, and, unlike the gai mutant, they were sensitive to GA treatment. 
Finally, it was shown that synergistic responses in the double mutant were not caused by elevated ethylene biosynthesis but that, in the light, enhanced sensitivity to ethylene may, at least in part, be responsible for the observed phenotype.},
  author       = {De Grauwe, Liesbeth and Chaerle, Laury and Dugardeyn, Jasper and Decat, Jan and Rieu, Ivo and Vriezen, Willem and Moritz, Thomas and Beemster, Gerrit and Phillips, Andy L and Harberd, Nicholas P and Hedden, Peter and Van Der Straeten, Dominique},
  issn         = {0028-646X},
  journal      = {NEW PHYTOLOGIST},
  language     = {eng},
  number       = {1},
  pages        = {128--141},
  title        = {Reduced gibberellin response affects ethylene biosynthesis and responsiveness in the Arabidopsis gai eto2-1 double mutant},
  url          = {http://dx.doi.org/10.1111/j.1469-8137.2007.02263.x},
  volume       = {177},
  year         = {2008},
}

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