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Cryptogein-induced transcriptional reprogramming in tobacco is light dependent

(2013) PLANT PHYSIOLOGY. 163(1). p.263-275
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
The fungal elicitor cryptogein triggers a light-dependent hypersensitive response in tobacco (Nicotiana tabacum). To assess the effect of light on this nonhost resistance in more detail, we studied various aspects of the response under dark and light conditions using the tobacco-cryptogein experimental system. Here, we show that light drastically alters the plant's transcriptional response to cryptogein, notably by dampening the induction of genes involved in multiple processes, such as ethylene biosynthesis, secondary metabolism, and glutathione turnover. Furthermore, chlorophyll fluorescence measurements demonstrated that quantum yield and functioning of the light-harvesting antennae decreased simultaneously, indicating that photoinhibition underlies the observed decreased photosynthesis and that photooxidative damage might be involved in the establishment of the altered response. Analysis of the isomer distribution of hydroxy fatty acids illustrated that, in the light, lipid peroxidation was predominantly due to the production of singlet oxygen. Differences in (reduced) glutathione concentrations and the rapid development of symptoms in the light when cryptogein was coinfiltrated with glutathione biosynthesis inhibitors suggest that glutathione might become a limiting factor during the cryptogein-induced hypersensitive response in the dark and that this response might be modified by an increased antioxidant availability in the light.
Keywords
PATHOGEN, CHLOROPHYLL FLUORESCENCE, SALICYLIC ACID, GENE-EXPRESSION, OXIDATIVE STRESS, PLANT DEFENSE, ACTIVATED PROTEIN KINASE, HYPERSENSITIVE CELL DEATH, FATTY ACID HYDROPEROXIDES, SYSTEMIC ACQUIRED RESISTANCE

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Citation

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MLA
Hoeberichts, Frank, Céline Davoine, Michaël Vandorpe, et al. “Cryptogein-induced Transcriptional Reprogramming in Tobacco Is Light Dependent.” PLANT PHYSIOLOGY 163.1 (2013): 263–275. Print.
APA
Hoeberichts, F., Davoine, C., Vandorpe, M., Morsa, S., Ksas, B., Stassen, C., Triantaphylidès, C., et al. (2013). Cryptogein-induced transcriptional reprogramming in tobacco is light dependent. PLANT PHYSIOLOGY, 163(1), 263–275.
Chicago author-date
Hoeberichts, Frank, Céline Davoine, Michaël Vandorpe, Stijn Morsa, Brigitte Ksas, Catherine Stassen, Christian Triantaphylidès, and Frank Van Breusegem. 2013. “Cryptogein-induced Transcriptional Reprogramming in Tobacco Is Light Dependent.” Plant Physiology 163 (1): 263–275.
Chicago author-date (all authors)
Hoeberichts, Frank, Céline Davoine, Michaël Vandorpe, Stijn Morsa, Brigitte Ksas, Catherine Stassen, Christian Triantaphylidès, and Frank Van Breusegem. 2013. “Cryptogein-induced Transcriptional Reprogramming in Tobacco Is Light Dependent.” Plant Physiology 163 (1): 263–275.
Vancouver
1.
Hoeberichts F, Davoine C, Vandorpe M, Morsa S, Ksas B, Stassen C, et al. Cryptogein-induced transcriptional reprogramming in tobacco is light dependent. PLANT PHYSIOLOGY. 2013;163(1):263–75.
IEEE
[1]
F. Hoeberichts et al., “Cryptogein-induced transcriptional reprogramming in tobacco is light dependent,” PLANT PHYSIOLOGY, vol. 163, no. 1, pp. 263–275, 2013.
@article{4143938,
  abstract     = {The fungal elicitor cryptogein triggers a light-dependent hypersensitive response in tobacco (Nicotiana tabacum). To assess the effect of light on this nonhost resistance in more detail, we studied various aspects of the response under dark and light conditions using the tobacco-cryptogein experimental system. Here, we show that light drastically alters the plant's transcriptional response to cryptogein, notably by dampening the induction of genes involved in multiple processes, such as ethylene biosynthesis, secondary metabolism, and glutathione turnover. Furthermore, chlorophyll fluorescence measurements demonstrated that quantum yield and functioning of the light-harvesting antennae decreased simultaneously, indicating that photoinhibition underlies the observed decreased photosynthesis and that photooxidative damage might be involved in the establishment of the altered response. Analysis of the isomer distribution of hydroxy fatty acids illustrated that, in the light, lipid peroxidation was predominantly due to the production of singlet oxygen. Differences in (reduced) glutathione concentrations and the rapid development of symptoms in the light when cryptogein was coinfiltrated with glutathione biosynthesis inhibitors suggest that glutathione might become a limiting factor during the cryptogein-induced hypersensitive response in the dark and that this response might be modified by an increased antioxidant availability in the light.},
  author       = {Hoeberichts, Frank and Davoine, Céline and Vandorpe, Michaël and Morsa, Stijn and Ksas, Brigitte and Stassen, Catherine and Triantaphylidès, Christian and Van Breusegem, Frank},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keywords     = {PATHOGEN,CHLOROPHYLL FLUORESCENCE,SALICYLIC ACID,GENE-EXPRESSION,OXIDATIVE STRESS,PLANT DEFENSE,ACTIVATED PROTEIN KINASE,HYPERSENSITIVE CELL DEATH,FATTY ACID HYDROPEROXIDES,SYSTEMIC ACQUIRED RESISTANCE},
  language     = {eng},
  number       = {1},
  pages        = {263--275},
  title        = {Cryptogein-induced transcriptional reprogramming in tobacco is light dependent},
  url          = {http://dx.doi.org/10.1104/pp.113.217240},
  volume       = {163},
  year         = {2013},
}

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