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A rapid test of alloreactivity based on interleukin-2 mRNA expression might identify liver transplant recipients with donor-specific hyporesponsiveness

(2007) TRANSPLANTATION PROCEEDINGS. 39(8). p.2665-2667
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Organization
Abstract
Background. Immunosuppression withdrawal is feasible in some liver transplant (OLT) recipients but may lead to severe rejection in others, underlying the need for reliable biomarkers to identify patients with tolerant profile in whose weaning/withdrawal could be safely proposed. We evaluated the value of real-time polymerase chain reaction (PCR)based measurement of interleukin (IL)-2 mRNA in mixed lymphocyte reaction (MLR) to monitor in vitro anti-donor reactivity in OLT patients. Methods. MLR were performed in three patients undergoing living donor OLT using a tolerogenic protocol including donor stem cells. IL-2 mRNA production in MLR was measured by PCR at several intervals after OLT. Results. In the early posttransplant period, three patients presented with global immunodeficiency, as indicated by low IL-2 mRNA production against both donor. and third-party antigens. In the two patients who has immunosuppression successfully withdrawn, donor-specific hyporesponsiveness was observed thereafter: IL-2 mRNA production against donor cells remained low, while IL-2 mRNA production against a third-party antigen-presenting cells progressively recovered. No such modulation of the anti-donor response was observed in the patient in whom withdrawal led to rapid rejection. Conclusion. Measurement of IL-2 mRNA production in MLR might preffer a tool to monitor anti-donor reactivity after OLT for decisions to minimize or withdraw immunosuppression in patients displaying donor-specific hyporesponsiveness.
Keywords
TOLERANCE, IMMUNOSUPPRESSION WITHDRAWAL, CHIMERISM, TRANSIENT

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MLA
Craciun, L, P Stordeur, Roberto Troisi, et al. “A Rapid Test of Alloreactivity Based on Interleukin-2 mRNA Expression Might Identify Liver Transplant Recipients with Donor-specific Hyporesponsiveness.” TRANSPLANTATION PROCEEDINGS 39.8 (2007): 2665–2667. Print.
APA
Craciun, L., Stordeur, P., Troisi, R., Le Moine, A., Toungouz, M., Colle, I., Van Vlierberghe, H., et al. (2007). A rapid test of alloreactivity based on interleukin-2 mRNA expression might identify liver transplant recipients with donor-specific hyporesponsiveness. TRANSPLANTATION PROCEEDINGS, 39(8), 2665–2667. Presented at the 14th Annual Meeting of the Belgian-Transplantation-Society.
Chicago author-date
Craciun, L, P Stordeur, Roberto Troisi, A Le Moine, M Toungouz, Isabelle Colle, Hans Van Vlierberghe, et al. 2007. “A Rapid Test of Alloreactivity Based on Interleukin-2 mRNA Expression Might Identify Liver Transplant Recipients with Donor-specific Hyporesponsiveness.” Transplantation Proceedings 39 (8): 2665–2667.
Chicago author-date (all authors)
Craciun, L, P Stordeur, Roberto Troisi, A Le Moine, M Toungouz, Isabelle Colle, Hans Van Vlierberghe, P Loi, V Lucidi, Marleen Praet, Bernard de Hemptinne, M Goldman, and V Donckier. 2007. “A Rapid Test of Alloreactivity Based on Interleukin-2 mRNA Expression Might Identify Liver Transplant Recipients with Donor-specific Hyporesponsiveness.” Transplantation Proceedings 39 (8): 2665–2667.
Vancouver
1.
Craciun L, Stordeur P, Troisi R, Le Moine A, Toungouz M, Colle I, et al. A rapid test of alloreactivity based on interleukin-2 mRNA expression might identify liver transplant recipients with donor-specific hyporesponsiveness. TRANSPLANTATION PROCEEDINGS. 2007;39(8):2665–7.
IEEE
[1]
L. Craciun et al., “A rapid test of alloreactivity based on interleukin-2 mRNA expression might identify liver transplant recipients with donor-specific hyporesponsiveness,” TRANSPLANTATION PROCEEDINGS, vol. 39, no. 8, pp. 2665–2667, 2007.
@article{414379,
  abstract     = {Background. Immunosuppression withdrawal is feasible in some liver transplant (OLT) recipients but may lead to severe rejection in others, underlying the need for reliable biomarkers to identify patients with tolerant profile in whose weaning/withdrawal could be safely proposed. We evaluated the value of real-time polymerase chain reaction (PCR)based measurement of interleukin (IL)-2 mRNA in mixed lymphocyte reaction (MLR) to monitor in vitro anti-donor reactivity in OLT patients.
Methods. MLR were performed in three patients undergoing living donor OLT using a tolerogenic protocol including donor stem cells. IL-2 mRNA production in MLR was measured by PCR at several intervals after OLT.
Results. In the early posttransplant period, three patients presented with global immunodeficiency, as indicated by low IL-2 mRNA production against both donor. and third-party antigens. In the two patients who has immunosuppression successfully withdrawn, donor-specific hyporesponsiveness was observed thereafter: IL-2 mRNA production against donor cells remained low, while IL-2 mRNA production against a third-party antigen-presenting cells progressively recovered. No such modulation of the anti-donor response was observed in the patient in whom withdrawal led to rapid rejection.
Conclusion. Measurement of IL-2 mRNA production in MLR might preffer a tool to monitor anti-donor reactivity after OLT for decisions to minimize or withdraw immunosuppression in patients displaying donor-specific hyporesponsiveness.},
  author       = {Craciun, L and Stordeur, P and Troisi, Roberto and Le Moine, A and Toungouz, M and Colle, Isabelle and Van Vlierberghe, Hans and Loi, P and Lucidi, V and Praet, Marleen and de Hemptinne, Bernard and Goldman, M and Donckier, V},
  issn         = {0041-1345},
  journal      = {TRANSPLANTATION PROCEEDINGS},
  keywords     = {TOLERANCE,IMMUNOSUPPRESSION WITHDRAWAL,CHIMERISM,TRANSIENT},
  language     = {eng},
  location     = {Brussels, Belgium},
  number       = {8},
  pages        = {2665--2667},
  title        = {A rapid test of alloreactivity based on interleukin-2 mRNA expression might identify liver transplant recipients with donor-specific hyporesponsiveness},
  url          = {http://dx.doi.org/10.1016/j.transproceed.2007.08.002},
  volume       = {39},
  year         = {2007},
}

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