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Virulence properties of Campylobacter jejuni isolates of poultry and human origin

Kim Van Deun UGent, Freddy Haesebrouck UGent, Marc Heyndrickx UGent, Herman Favoreel UGent, Jeroen Dewulf UGent, Liesbeth Ceelen, Linn Dumez UGent, Winy Messens UGent, Saskia Leleu, Filip Van Immerseel UGent, et al. (2007) JOURNAL OF MEDICAL MICROBIOLOGY. 56(10). p.1284-1289
abstract
Campylobacter jejuni is one of the leading causes of food-borne gastroenteritis. Because of the high prevalence of C. jejuni in poultry, poultry meat is considered a major source of C. jejuni infections for humans. However, it is not known whether all poultry-associated C. jejuni strains are capable of causing disease in humans. Four different virulence properties of C. jejuni strains were compared between 20 poultry isolates and 24 human isolates. Strains were chosen based on their PFGE pattern to represent a heterogeneous population. The isolates were compared for their ability to invade and induce interleukin-8 (IL-8) production in T84 cells, their production of functional cytolethal distending toxin (CDT) using HEp-2 cells, and their sodium deoxycholate resistance. All four virulence factors were present among strains of human and poultry origin, with strong differences observed among strains. For invasion and IL-8 induction, no difference was observed between the two populations. However, on average, human isolates arrested more HEp-2 cells in their cell cycle than did the poultry isolates (P=0.041), suggesting higher CDT production by the former. The ability to survive 16 000 mu g sodium deoxycholate ml(-1) was significantly more pronounced (P=0.006) among human isolates than poultry isolates, although all strains possessed the cmeABC operon. These data suggest that all four virulence properties are widespread among C. jejuni isolates, but that a higher degree of bile-salt resistance and more pronounced CDT production are associated with strains causing enteritis in humans.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PREVALENCE, INVASION, CYTOLETHAL, IN-VITRO, CDT GENES, HEP-2 CELLS, DISTENDING TOXIN PRODUCTION, CHICKEN CARCASSES, INTERLEUKIN-8, COLONIZATION
journal title
JOURNAL OF MEDICAL MICROBIOLOGY
J. Med. Microbiol.
volume
56
issue
10
pages
1284-1289 pages
publisher
Society for General Microbiology
place of publication
Reading, UK
Web of Science type
Article
Web of Science id
000250538100004
JCR category
MICROBIOLOGY
JCR impact factor
2.091 (2007)
JCR rank
50/91 (2007)
JCR quartile
3 (2007)
ISSN
0022-2615
DOI
10.1099/jmm.0.47342-0
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
414367
handle
http://hdl.handle.net/1854/LU-414367
date created
2007-12-06 17:07:00
date last changed
2017-01-02 09:55:54
@article{414367,
  abstract     = {Campylobacter jejuni is one of the leading causes of food-borne gastroenteritis. Because of the high prevalence of C. jejuni in poultry, poultry meat is considered a major source of C. jejuni infections for humans. However, it is not known whether all poultry-associated C. jejuni strains are capable of causing disease in humans. Four different virulence properties of C. jejuni strains were compared between 20 poultry isolates and 24 human isolates. Strains were chosen based on their PFGE pattern to represent a heterogeneous population. The isolates were compared for their ability to invade and induce interleukin-8 (IL-8) production in T84 cells, their production of functional cytolethal distending toxin (CDT) using HEp-2 cells, and their sodium deoxycholate resistance. All four virulence factors were present among strains of human and poultry origin, with strong differences observed among strains. For invasion and IL-8 induction, no difference was observed between the two populations. However, on average, human isolates arrested more HEp-2 cells in their cell cycle than did the poultry isolates (P=0.041), suggesting higher CDT production by the former. The ability to survive 16 000 mu g sodium deoxycholate ml(-1) was significantly more pronounced (P=0.006) among human isolates than poultry isolates, although all strains possessed the cmeABC operon. These data suggest that all four virulence properties are widespread among C. jejuni isolates, but that a higher degree of bile-salt resistance and more pronounced CDT production are associated with strains causing enteritis in humans.},
  author       = {Van Deun, Kim and Haesebrouck, Freddy and Heyndrickx, Marc and Favoreel, Herman and Dewulf, Jeroen and Ceelen, Liesbeth and Dumez, Linn and Messens, Winy and Leleu, Saskia and Van Immerseel, Filip and Ducatelle, Richard and Pasmans, Frank},
  issn         = {0022-2615},
  journal      = {JOURNAL OF MEDICAL MICROBIOLOGY},
  keyword      = {PREVALENCE,INVASION,CYTOLETHAL,IN-VITRO,CDT GENES,HEP-2 CELLS,DISTENDING TOXIN PRODUCTION,CHICKEN CARCASSES,INTERLEUKIN-8,COLONIZATION},
  language     = {eng},
  number       = {10},
  pages        = {1284--1289},
  publisher    = {Society for General Microbiology},
  title        = {Virulence properties of Campylobacter jejuni isolates of poultry and human origin},
  url          = {http://dx.doi.org/10.1099/jmm.0.47342-0},
  volume       = {56},
  year         = {2007},
}

Chicago
Van Deun, Kim, Freddy Haesebrouck, Marc Heyndrickx, Herman Favoreel, Jeroen Dewulf, Liesbeth Ceelen, Linn Dumez, et al. 2007. “Virulence Properties of Campylobacter Jejuni Isolates of Poultry and Human Origin.” Journal of Medical Microbiology 56 (10): 1284–1289.
APA
Van Deun, Kim, Haesebrouck, F., Heyndrickx, M., Favoreel, H., Dewulf, J., Ceelen, L., Dumez, L., et al. (2007). Virulence properties of Campylobacter jejuni isolates of poultry and human origin. JOURNAL OF MEDICAL MICROBIOLOGY, 56(10), 1284–1289.
Vancouver
1.
Van Deun K, Haesebrouck F, Heyndrickx M, Favoreel H, Dewulf J, Ceelen L, et al. Virulence properties of Campylobacter jejuni isolates of poultry and human origin. JOURNAL OF MEDICAL MICROBIOLOGY. Reading, UK: Society for General Microbiology; 2007;56(10):1284–9.
MLA
Van Deun, Kim, Freddy Haesebrouck, Marc Heyndrickx, et al. “Virulence Properties of Campylobacter Jejuni Isolates of Poultry and Human Origin.” JOURNAL OF MEDICAL MICROBIOLOGY 56.10 (2007): 1284–1289. Print.