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Novel plant-specific cyclin-dependent kinase inhibitors induced by biotic and abiotic stresses

(2007) JOURNAL OF BIOLOGICAL CHEMISTRY. 282(35). p.25588-25596
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Abstract
The EL2 gene of rice ( Oryza sativa), previously classified as early response gene against the potent biotic elicitor N-acetylchitoheptaose and encoding a short polypeptide with unknown function, was identified as a novel cell cycle regulatory gene related to the recently reported SIAMESE ( SIM) gene of Arabidopsis thaliana. Iterative two-hybrid screens, in vitro pull-down assays, and fluorescence resonance energy transfer analyses showed that Orysa; EL2 binds the cyclin-dependent kinase ( CDK) CDKA1; 1 and D-type cyclins. No interaction was observed with the plant-specific B-type CDKs. The amino acid motif ELERFL was identified to be essential for cyclin, but not for CDK binding. Orysa; EL2 impaired the ability of Orysa; CYCD5; 3 to complement a budding yeast ( Saccharomyces cerevisiae) triple CLN mutant, whereas recombinant protein inhibited CDK activity in vitro. Moreover, Orysa; EL2 was able to rescue the multicellular trichome phenotype of sim mutants of Arabidopsis, unequivocally demonstrating that Orysa; EL2 operates as a cell cycle inhibitor. Orysa; EL2 mRNA levels were induced by cold, drought, and propionic acid. Our data suggest that Orysa; EL2 encodes a new type of plant CDK inhibitor that links cell cycle progression with biotic and abiotic stress responses.
Keywords
JASMONIC ACID, SALT STRESS, ABSCISIC-ACID, GENE-EXPRESSION, PROTEIN-INTERACTION, N-ACETYLCHITOHEPTAOSE, ARABIDOPSIS-THALIANA, GENOME-WIDE ANALYSIS, TOBACCO BY-2 CELLS, CULTURED RICE CELLS

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Citation

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Chicago
Peres, Adrian, Michelle L Churchman, Srivaidehirani Hariharan, Kristiina Himanen, Aurine Verkest, Klaas Vandepoele, Zoltán Magyar, et al. 2007. “Novel Plant-specific Cyclin-dependent Kinase Inhibitors Induced by Biotic and Abiotic Stresses.” Journal of Biological Chemistry 282 (35): 25588–25596.
APA
Peres, A., Churchman, M. L., Hariharan, S., Himanen, K., Verkest, A., Vandepoele, K., Magyar, Z., et al. (2007). Novel plant-specific cyclin-dependent kinase inhibitors induced by biotic and abiotic stresses. JOURNAL OF BIOLOGICAL CHEMISTRY, 282(35), 25588–25596.
Vancouver
1.
Peres A, Churchman ML, Hariharan S, Himanen K, Verkest A, Vandepoele K, et al. Novel plant-specific cyclin-dependent kinase inhibitors induced by biotic and abiotic stresses. JOURNAL OF BIOLOGICAL CHEMISTRY. 2007;282(35):25588–96.
MLA
Peres, Adrian, Michelle L Churchman, Srivaidehirani Hariharan, et al. “Novel Plant-specific Cyclin-dependent Kinase Inhibitors Induced by Biotic and Abiotic Stresses.” JOURNAL OF BIOLOGICAL CHEMISTRY 282.35 (2007): 25588–25596. Print.
@article{414197,
  abstract     = {The EL2 gene of rice ( Oryza sativa), previously classified as early response gene against the potent biotic elicitor N-acetylchitoheptaose and encoding a short polypeptide with unknown function, was identified as a novel cell cycle regulatory gene related to the recently reported SIAMESE ( SIM) gene of Arabidopsis thaliana. Iterative two-hybrid screens, in vitro pull-down assays, and fluorescence resonance energy transfer analyses showed that Orysa; EL2 binds the cyclin-dependent kinase ( CDK) CDKA1; 1 and D-type cyclins. No interaction was observed with the plant-specific B-type CDKs. The amino acid motif ELERFL was identified to be essential for cyclin, but not for CDK binding. Orysa; EL2 impaired the ability of Orysa; CYCD5; 3 to complement a budding yeast ( Saccharomyces cerevisiae) triple CLN mutant, whereas recombinant protein inhibited CDK activity in vitro. Moreover, Orysa; EL2 was able to rescue the multicellular trichome phenotype of sim mutants of Arabidopsis, unequivocally demonstrating that Orysa; EL2 operates as a cell cycle inhibitor. Orysa; EL2 mRNA levels were induced by cold, drought, and propionic acid. Our data suggest that Orysa; EL2 encodes a new type of plant CDK inhibitor that links cell cycle progression with biotic and abiotic stress responses.},
  author       = {Peres, Adrian and Churchman, Michelle L and Hariharan, Srivaidehirani and Himanen, Kristiina and Verkest, Aurine and Vandepoele, Klaas and Magyar, Zolt{\'a}n and Hatzfeld, Yves and Van Der Schueren, Els and Beemster, Gerrit and Frankard, Valerie and Larkin, John C and Inz{\'e}, Dirk and De Veylder, Lieven},
  issn         = {0021-9258},
  journal      = {JOURNAL OF BIOLOGICAL CHEMISTRY},
  keyword      = {JASMONIC ACID,SALT STRESS,ABSCISIC-ACID,GENE-EXPRESSION,PROTEIN-INTERACTION,N-ACETYLCHITOHEPTAOSE,ARABIDOPSIS-THALIANA,GENOME-WIDE ANALYSIS,TOBACCO BY-2 CELLS,CULTURED RICE CELLS},
  language     = {eng},
  number       = {35},
  pages        = {25588--25596},
  title        = {Novel plant-specific cyclin-dependent kinase inhibitors induced by biotic and abiotic stresses},
  url          = {http://dx.doi.org/10.1074/jbc.M703326200},
  volume       = {282},
  year         = {2007},
}

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