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Novel plant-specific cyclin-dependent kinase inhibitors induced by biotic and abiotic stresses

Adrian Peres, Michelle L Churchman, Srivaidehirani Hariharan UGent, Kristiina Himanen UGent, Aurine Verkest, Klaas Vandepoele UGent, Zoltán Magyar UGent, Yves Hatzfeld, Els Van Der Schueren, Gerrit Beemster UGent, et al. (2007) JOURNAL OF BIOLOGICAL CHEMISTRY. 282(35). p.25588-25596
abstract
The EL2 gene of rice ( Oryza sativa), previously classified as early response gene against the potent biotic elicitor N-acetylchitoheptaose and encoding a short polypeptide with unknown function, was identified as a novel cell cycle regulatory gene related to the recently reported SIAMESE ( SIM) gene of Arabidopsis thaliana. Iterative two-hybrid screens, in vitro pull-down assays, and fluorescence resonance energy transfer analyses showed that Orysa; EL2 binds the cyclin-dependent kinase ( CDK) CDKA1; 1 and D-type cyclins. No interaction was observed with the plant-specific B-type CDKs. The amino acid motif ELERFL was identified to be essential for cyclin, but not for CDK binding. Orysa; EL2 impaired the ability of Orysa; CYCD5; 3 to complement a budding yeast ( Saccharomyces cerevisiae) triple CLN mutant, whereas recombinant protein inhibited CDK activity in vitro. Moreover, Orysa; EL2 was able to rescue the multicellular trichome phenotype of sim mutants of Arabidopsis, unequivocally demonstrating that Orysa; EL2 operates as a cell cycle inhibitor. Orysa; EL2 mRNA levels were induced by cold, drought, and propionic acid. Our data suggest that Orysa; EL2 encodes a new type of plant CDK inhibitor that links cell cycle progression with biotic and abiotic stress responses.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
JASMONIC ACID, SALT STRESS, ABSCISIC-ACID, GENE-EXPRESSION, PROTEIN-INTERACTION, N-ACETYLCHITOHEPTAOSE, ARABIDOPSIS-THALIANA, GENOME-WIDE ANALYSIS, TOBACCO BY-2 CELLS, CULTURED RICE CELLS
journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
J. Biol. Chem.
volume
282
issue
35
pages
25588 - 25596
Web of Science type
Article
Web of Science id
000249014100045
JCR category
BIOCHEMISTRY & MOLECULAR BIOLOGY
JCR impact factor
5.581 (2007)
JCR rank
40/260 (2007)
JCR quartile
1 (2007)
ISSN
0021-9258
DOI
10.1074/jbc.M703326200
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
414197
handle
http://hdl.handle.net/1854/LU-414197
date created
2008-05-14 17:28:00
date last changed
2016-12-19 15:43:13
@article{414197,
  abstract     = {The EL2 gene of rice ( Oryza sativa), previously classified as early response gene against the potent biotic elicitor N-acetylchitoheptaose and encoding a short polypeptide with unknown function, was identified as a novel cell cycle regulatory gene related to the recently reported SIAMESE ( SIM) gene of Arabidopsis thaliana. Iterative two-hybrid screens, in vitro pull-down assays, and fluorescence resonance energy transfer analyses showed that Orysa; EL2 binds the cyclin-dependent kinase ( CDK) CDKA1; 1 and D-type cyclins. No interaction was observed with the plant-specific B-type CDKs. The amino acid motif ELERFL was identified to be essential for cyclin, but not for CDK binding. Orysa; EL2 impaired the ability of Orysa; CYCD5; 3 to complement a budding yeast ( Saccharomyces cerevisiae) triple CLN mutant, whereas recombinant protein inhibited CDK activity in vitro. Moreover, Orysa; EL2 was able to rescue the multicellular trichome phenotype of sim mutants of Arabidopsis, unequivocally demonstrating that Orysa; EL2 operates as a cell cycle inhibitor. Orysa; EL2 mRNA levels were induced by cold, drought, and propionic acid. Our data suggest that Orysa; EL2 encodes a new type of plant CDK inhibitor that links cell cycle progression with biotic and abiotic stress responses.},
  author       = {Peres, Adrian and Churchman, Michelle L and Hariharan, Srivaidehirani and Himanen, Kristiina and Verkest, Aurine and Vandepoele, Klaas and Magyar, Zolt{\'a}n and Hatzfeld, Yves and Van Der Schueren, Els and Beemster, Gerrit and Frankard, Valerie and Larkin, John C and Inz{\'e}, Dirk and De Veylder, Lieven},
  issn         = {0021-9258},
  journal      = {JOURNAL OF BIOLOGICAL CHEMISTRY},
  keyword      = {JASMONIC ACID,SALT STRESS,ABSCISIC-ACID,GENE-EXPRESSION,PROTEIN-INTERACTION,N-ACETYLCHITOHEPTAOSE,ARABIDOPSIS-THALIANA,GENOME-WIDE ANALYSIS,TOBACCO BY-2 CELLS,CULTURED RICE CELLS},
  language     = {eng},
  number       = {35},
  pages        = {25588--25596},
  title        = {Novel plant-specific cyclin-dependent kinase inhibitors induced by biotic and abiotic stresses},
  url          = {http://dx.doi.org/10.1074/jbc.M703326200},
  volume       = {282},
  year         = {2007},
}

Chicago
Peres, Adrian, Michelle L Churchman, Srivaidehirani Hariharan, Kristiina Himanen, Aurine Verkest, Klaas Vandepoele, Zoltán Magyar, et al. 2007. “Novel Plant-specific Cyclin-dependent Kinase Inhibitors Induced by Biotic and Abiotic Stresses.” Journal of Biological Chemistry 282 (35): 25588–25596.
APA
Peres, A., Churchman, M. L., Hariharan, S., Himanen, K., Verkest, A., Vandepoele, K., Magyar, Z., et al. (2007). Novel plant-specific cyclin-dependent kinase inhibitors induced by biotic and abiotic stresses. JOURNAL OF BIOLOGICAL CHEMISTRY, 282(35), 25588–25596.
Vancouver
1.
Peres A, Churchman ML, Hariharan S, Himanen K, Verkest A, Vandepoele K, et al. Novel plant-specific cyclin-dependent kinase inhibitors induced by biotic and abiotic stresses. JOURNAL OF BIOLOGICAL CHEMISTRY. 2007;282(35):25588–96.
MLA
Peres, Adrian, Michelle L Churchman, Srivaidehirani Hariharan, et al. “Novel Plant-specific Cyclin-dependent Kinase Inhibitors Induced by Biotic and Abiotic Stresses.” JOURNAL OF BIOLOGICAL CHEMISTRY 282.35 (2007): 25588–25596. Print.