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Development of a sensitive and accurate stable isotope dilution assay for the simultaneous determination of free 4-hydroxy-2-(E)-nonenal and 4-hydroxy-2-(E)-hexenal in various food matrices by gas chromatography–mass spectrometry

(2014) FOOD ANALYTICAL METHODS. 7(4). p.836-843
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Organization
Abstract
An analytical method suitable for the determination of 4-hydroxy-2-(E)-nonenal (HNE) and 4-hydroxy-2-(E)-hexenal (HHE) in various food matrices was developed and validated. The method involves the use of deuterated HNE and HHE as internal standards, extraction of the analytes from the matrices followed by derivatization and detection with gas chromatography–mass spectrometry. Four different food matrices were chosen as model systems including vegetable oils, unprocessed meat, fried potato crisps, and infant formula and three different extraction techniques suitable for the different matrices were applied including the Quick Easy Cheap Effective Rugged Safe method. The simplicity of the extraction techniques allows the method to be applied for routine analysis of a large amount of samples. The results verify the accuracy and reproducibility of the analytical technique and its ability to provide reliable quantification of both analytes at concentrations as low as 12.8 ng g−1 in meat samples. Furthermore, a short overview of the levels of HNE and HHE in several products available in the Belgian market is presented.
Keywords
Hydroxy-2-(E)-hexenal, 4-Hydroxy-2-(E)-nonenal, QuEchERS, Lipid oxidation, POLYUNSATURATED FATTY-ACIDS, OXYGENATED ALPHA, BETA-UNSATURATED ALDEHYDES, SOLID-PHASE EXTRACTION, LIQUID-CHROMATOGRAPHY, IN-VITRO, MULTIRESIDUE METHOD, FRYING TEMPERATURE, OXIDATION-PRODUCTS, PESTICIDE-RESIDUES, LIPID OXIDATION

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Citation

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MLA
Papastergiadis, Antonios, Edward Mubiru, Herman Van Langenhove, et al. “Development of a Sensitive and Accurate Stable Isotope Dilution Assay for the Simultaneous Determination of Free 4-hydroxy-2-(E)-nonenal and 4-hydroxy-2-(E)-hexenal in Various Food Matrices by Gas Chromatography–mass Spectrometry.” FOOD ANALYTICAL METHODS 7.4 (2014): 836–843. Print.
APA
Papastergiadis, A., Mubiru, E., Van Langenhove, H., & De Meulenaer, B. (2014). Development of a sensitive and accurate stable isotope dilution assay for the simultaneous determination of free 4-hydroxy-2-(E)-nonenal and 4-hydroxy-2-(E)-hexenal in various food matrices by gas chromatography–mass spectrometry. FOOD ANALYTICAL METHODS, 7(4), 836–843.
Chicago author-date
Papastergiadis, Antonios, Edward Mubiru, Herman Van Langenhove, and Bruno De Meulenaer. 2014. “Development of a Sensitive and Accurate Stable Isotope Dilution Assay for the Simultaneous Determination of Free 4-hydroxy-2-(E)-nonenal and 4-hydroxy-2-(E)-hexenal in Various Food Matrices by Gas Chromatography–mass Spectrometry.” Food Analytical Methods 7 (4): 836–843.
Chicago author-date (all authors)
Papastergiadis, Antonios, Edward Mubiru, Herman Van Langenhove, and Bruno De Meulenaer. 2014. “Development of a Sensitive and Accurate Stable Isotope Dilution Assay for the Simultaneous Determination of Free 4-hydroxy-2-(E)-nonenal and 4-hydroxy-2-(E)-hexenal in Various Food Matrices by Gas Chromatography–mass Spectrometry.” Food Analytical Methods 7 (4): 836–843.
Vancouver
1.
Papastergiadis A, Mubiru E, Van Langenhove H, De Meulenaer B. Development of a sensitive and accurate stable isotope dilution assay for the simultaneous determination of free 4-hydroxy-2-(E)-nonenal and 4-hydroxy-2-(E)-hexenal in various food matrices by gas chromatography–mass spectrometry. FOOD ANALYTICAL METHODS. 2014;7(4):836–43.
IEEE
[1]
A. Papastergiadis, E. Mubiru, H. Van Langenhove, and B. De Meulenaer, “Development of a sensitive and accurate stable isotope dilution assay for the simultaneous determination of free 4-hydroxy-2-(E)-nonenal and 4-hydroxy-2-(E)-hexenal in various food matrices by gas chromatography–mass spectrometry,” FOOD ANALYTICAL METHODS, vol. 7, no. 4, pp. 836–843, 2014.
@article{4141965,
  abstract     = {An analytical method suitable for the determination of 4-hydroxy-2-(E)-nonenal (HNE) and 4-hydroxy-2-(E)-hexenal (HHE) in various food matrices was developed and validated. The method involves the use of deuterated HNE and HHE as internal standards, extraction of the analytes from the matrices followed by derivatization and detection with gas chromatography–mass spectrometry. Four different food matrices were chosen as model systems including vegetable oils, unprocessed meat, fried potato crisps, and infant formula and three different extraction techniques suitable for the different matrices were applied including the Quick Easy Cheap Effective Rugged Safe method. The simplicity of the extraction techniques allows the method to be applied for routine analysis of a large amount of samples. The results verify the accuracy and reproducibility of the analytical technique and its ability to provide reliable quantification of both analytes at concentrations as low as 12.8 ng g−1 in meat samples. Furthermore, a short overview of the levels of HNE and HHE in several products available in the Belgian market is presented.},
  author       = {Papastergiadis, Antonios and Mubiru, Edward and Van Langenhove, Herman and De Meulenaer, Bruno},
  issn         = {1936-9751},
  journal      = {FOOD ANALYTICAL METHODS},
  keywords     = {Hydroxy-2-(E)-hexenal,4-Hydroxy-2-(E)-nonenal,QuEchERS,Lipid oxidation,POLYUNSATURATED FATTY-ACIDS,OXYGENATED ALPHA,BETA-UNSATURATED ALDEHYDES,SOLID-PHASE EXTRACTION,LIQUID-CHROMATOGRAPHY,IN-VITRO,MULTIRESIDUE METHOD,FRYING TEMPERATURE,OXIDATION-PRODUCTS,PESTICIDE-RESIDUES,LIPID OXIDATION},
  language     = {eng},
  number       = {4},
  pages        = {836--843},
  title        = {Development of a sensitive and accurate stable isotope dilution assay for the simultaneous determination of free 4-hydroxy-2-(E)-nonenal and 4-hydroxy-2-(E)-hexenal in various food matrices by gas chromatography–mass spectrometry},
  url          = {http://dx.doi.org/10.1007/s12161-013-9689-2},
  volume       = {7},
  year         = {2014},
}

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