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Molecular-based identification of Sarcocystis hominis in Belgian minced beef

Lieve Vangeel UGent, Kurt Houf UGent, Koen Chiers UGent, Jozef Vercruysse UGent, Katharina D'Herde UGent and Richard Ducatelle UGent (2007) JOURNAL OF FOOD PROTECTION. 70(6). p.1523-1526
abstract
Sarcocystis hominis, one of the three species of Sarcocystis that cause muscular cysts in cattle, is a protozoan parasite that can infect the human intestinal tract. The objective of the present study was to develop a new molecular identification method capable of discriminating among the bovine Sarcocystis species and to apply this tool in combination with stereo-microscopy to determine the presence of Sarcocystis spp. in minced beef in Belgium, with special attention to Sarcocystis hominis. A PCR technique based on the 18S rRNA sequence and by sequencing of the amplicon was highly specific. Sequence analysis of PCR products from thick.-walled cysts collected from minced beef in Belgium revealed that S. hominis was present in 97.4% of the samples. Because the consumption of raw minced beef is common in Belgium and certain other European countries, these findings may point to an underestimated risk to public health.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
COCCIDIA, SPP., DOMESTIC-ANIMALS, TRANSMISSION, PREVALENCE, CATTLE, IDENTITY, MEAT, INFECTIONS
journal title
JOURNAL OF FOOD PROTECTION
J. Food Prot.
volume
70
issue
6
pages
1523-1526 pages
Web of Science type
Article
Web of Science id
000247090600031
JCR category
FOOD SCIENCE & TECHNOLOGY
JCR impact factor
1.886 (2007)
JCR rank
22/102 (2007)
JCR quartile
1 (2007)
ISSN
0362-028X
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
414011
handle
http://hdl.handle.net/1854/LU-414011
date created
2007-08-21 12:26:00
date last changed
2010-06-22 13:22:01
@article{414011,
  abstract     = {Sarcocystis hominis, one of the three species of Sarcocystis that cause muscular cysts in cattle, is a protozoan parasite that can infect the human intestinal tract. The objective of the present study was to develop a new molecular identification method capable of discriminating among the bovine Sarcocystis species and to apply this tool in combination with stereo-microscopy to determine the presence of Sarcocystis spp. in minced beef in Belgium, with special attention to Sarcocystis hominis. A PCR technique based on the 18S rRNA sequence and by sequencing of the amplicon was highly specific. Sequence analysis of PCR products from thick.-walled cysts collected from minced beef in Belgium revealed that S. hominis was present in 97.4\% of the samples. Because the consumption of raw minced beef is common in Belgium and certain other European countries, these findings may point to an underestimated risk to public health.},
  author       = {Vangeel, Lieve and Houf, Kurt and Chiers, Koen and Vercruysse, Jozef and D'Herde, Katharina and Ducatelle, Richard},
  issn         = {0362-028X},
  journal      = {JOURNAL OF FOOD PROTECTION},
  keyword      = {COCCIDIA,SPP.,DOMESTIC-ANIMALS,TRANSMISSION,PREVALENCE,CATTLE,IDENTITY,MEAT,INFECTIONS},
  language     = {eng},
  number       = {6},
  pages        = {1523--1526},
  title        = {Molecular-based identification of Sarcocystis hominis in Belgian minced beef},
  volume       = {70},
  year         = {2007},
}

Chicago
Vangeel, Lieve, Kurt Houf, Koen Chiers, Jozef Vercruysse, Katharina D’Herde, and Richard Ducatelle. 2007. “Molecular-based Identification of Sarcocystis Hominis in Belgian Minced Beef.” Journal of Food Protection 70 (6): 1523–1526.
APA
Vangeel, L., Houf, K., Chiers, K., Vercruysse, J., D’Herde, K., & Ducatelle, R. (2007). Molecular-based identification of Sarcocystis hominis in Belgian minced beef. JOURNAL OF FOOD PROTECTION, 70(6), 1523–1526.
Vancouver
1.
Vangeel L, Houf K, Chiers K, Vercruysse J, D’Herde K, Ducatelle R. Molecular-based identification of Sarcocystis hominis in Belgian minced beef. JOURNAL OF FOOD PROTECTION. 2007;70(6):1523–6.
MLA
Vangeel, Lieve, Kurt Houf, Koen Chiers, et al. “Molecular-based Identification of Sarcocystis Hominis in Belgian Minced Beef.” JOURNAL OF FOOD PROTECTION 70.6 (2007): 1523–1526. Print.