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The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth

Delphine Fleury, Kristiina Himanen UGent, Gerda Cnops UGent, Hilde Nelissen UGent, Tommaso Matteo Boccardi UGent, Steven Maere UGent, Gerrit Beemster UGent, Pia Neyt UGent, Sylvester Anami and Pedro Robles, et al. (2007) PLANT CELL. 19(2). p.417-432
abstract
Chromatin modification and transcriptional activation are novel roles for E3 ubiquitin ligase proteins that have been mainly associated with ubiquitin-dependent proteolysis. We identified HISTONE MONOUBIQUITINATION1 (HUB1) (and its homolog HUB2) in Arabidopsis thaliana as RING E3 ligase proteins with a function in organ growth. We show that HUB1 is a functional homolog of the human and yeast BRE1 proteins because it monoubiquitinated histone H2B in an in vitro assay. Hub knockdown mutants had pale leaf coloration, modified leaf shape, reduced rosette biomass, and inhibited primary root growth. One of the alleles had been designated previously as ang4-1. Kinematic analysis of leaf and root growth together with flow cytometry revealed defects in cell cycle activities. The hub1-1 (ang4-1) mutation increased cell cycle duration in young leaves and caused an early entry into the endocycles. Transcript profiling of shoot apical tissues of hub1-1 (ang4-1) indicated that key regulators of the G2-to-M transition were misexpressed. Based on the mutant characterization, we postulate that HUB1 mediates gene activation and cell cycle regulation probably through chromatin modifications.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GENE-EXPRESSION, ORGAN GROWTH, MUTANTS IDENTIFY, PLANT DEVELOPMENT, MEDIATED TRANSFORMATION, HISTONE H2B MONOUBIQUITINATION, RNA-POLYMERASE-II, SIZE CONTROL, UBIQUITIN, PROTEIN
journal title
PLANT CELL
Plant Cell
volume
19
issue
2
pages
417 - 432
Web of Science type
Article
Web of Science id
000245467700007
JCR category
PLANT SCIENCES
JCR impact factor
9.653 (2007)
JCR rank
3/149 (2007)
JCR quartile
1 (2007)
ISSN
1040-4651
DOI
10.1105/tpc.106.041319
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
413828
handle
http://hdl.handle.net/1854/LU-413828
date created
2008-05-15 08:16:00
date last changed
2012-12-18 13:48:52
@article{413828,
  abstract     = {Chromatin modification and transcriptional activation are novel roles for E3 ubiquitin ligase proteins that have been mainly associated with ubiquitin-dependent proteolysis. We identified HISTONE MONOUBIQUITINATION1 (HUB1) (and its homolog HUB2) in Arabidopsis thaliana as RING E3 ligase proteins with a function in organ growth. We show that HUB1 is a functional homolog of the human and yeast BRE1 proteins because it monoubiquitinated histone H2B in an in vitro assay. Hub knockdown mutants had pale leaf coloration, modified leaf shape, reduced rosette biomass, and inhibited primary root growth. One of the alleles had been designated previously as ang4-1. Kinematic analysis of leaf and root growth together with flow cytometry revealed defects in cell cycle activities. The hub1-1 (ang4-1) mutation increased cell cycle duration in young leaves and caused an early entry into the endocycles. Transcript profiling of shoot apical tissues of hub1-1 (ang4-1) indicated that key regulators of the G2-to-M transition were misexpressed. Based on the mutant characterization, we postulate that HUB1 mediates gene activation and cell cycle regulation probably through chromatin modifications.},
  author       = {Fleury, Delphine and Himanen, Kristiina and Cnops, Gerda and Nelissen, Hilde and Boccardi, Tommaso Matteo and Maere, Steven and Beemster, Gerrit and Neyt, Pia and Anami, Sylvester and Robles, Pedro and Micol, Jos{\'e} Luis and Inz{\'e}, Dirk and Van Lijsebettens, Maria},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {GENE-EXPRESSION,ORGAN GROWTH,MUTANTS IDENTIFY,PLANT DEVELOPMENT,MEDIATED TRANSFORMATION,HISTONE H2B MONOUBIQUITINATION,RNA-POLYMERASE-II,SIZE CONTROL,UBIQUITIN,PROTEIN},
  language     = {eng},
  number       = {2},
  pages        = {417--432},
  title        = {The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth},
  url          = {http://dx.doi.org/10.1105/tpc.106.041319},
  volume       = {19},
  year         = {2007},
}

Chicago
Fleury, Delphine, Kristiina Himanen, Gerda Cnops, Hilde Nelissen, Tommaso Matteo Boccardi, Steven Maere, Gerrit Beemster, et al. 2007. “The Arabidopsis Thaliana Homolog of Yeast BRE1 Has a Function in Cell Cycle Regulation During Early Leaf and Root Growth.” Plant Cell 19 (2): 417–432.
APA
Fleury, D., Himanen, K., Cnops, G., Nelissen, H., Boccardi, T. M., Maere, S., Beemster, G., et al. (2007). The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth. PLANT CELL, 19(2), 417–432.
Vancouver
1.
Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, et al. The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth. PLANT CELL. 2007;19(2):417–32.
MLA
Fleury, Delphine, Kristiina Himanen, Gerda Cnops, et al. “The Arabidopsis Thaliana Homolog of Yeast BRE1 Has a Function in Cell Cycle Regulation During Early Leaf and Root Growth.” PLANT CELL 19.2 (2007): 417–432. Print.