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Genome wide measurement of DNA copy number changes in neuroblastoma: dissecting amplicons and mapping losses, gains and breakpoints

Evi Michels (UGent), Jo Vandesompele (UGent), Jasmien Hoebeeck (UGent), Björn Menten (UGent), Katleen De Preter (UGent), Genevieve Laureys (UGent), Nadine Van Roy (UGent) and Franki Speleman (UGent)
(2006) CYTOGENETIC AND GENOME RESEARCH. 115(3-4). p.273-282
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Abstract
In the past few years high throughput methods for assessment of DNA copy number alterations have witnessed rapid progress. Both 'in house' developed BAC, cDNA, oligonucleotide and commercial arrays are now available and widely applied in the study of the human genome, particularly in the context of disease. Cancer cells are known to exhibit DNA losses, gains and amplifications affecting tumor suppressor genes and proto-oncogenes. Moreover, these patterns of genomic imbalances may be associated with particular tumor types or subtypes and may have prognostic value. Here we summarize recent array CGH findings in neuroblastoma, a pediatric tumor of the sympathetic nervous system. A total of 176 primary tumors and 53 cell lines have been analyzed on different platforms. Through these studies the genomic content and boundaries of deletions, gains and amplifications were characterized with unprecedented accuracy. Furthermore, in conjunction with cytogenetic findings, array CGH allows the mapping of breakpoints of unbalanced translocations at a very high resolution.
Keywords
HYBRIDIZATION CGH ANALYSIS, HIGH-RESOLUTION ANALYSIS, ACUTE MYELOID LEUKEMIAS, TUMOR-SUPPRESSOR GENE, HUMAN NEURO-BLASTOMA, COMMON ALLELIC LOSS, CELL-LINES, ARRAY-CGH, N-MYC, CYTOGENETIC ANALYSIS

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Chicago
Michels, Evi, Jo Vandesompele, Jasmien Hoebeeck, Björn Menten, Katleen De Preter, Genevieve Laureys, Nadine Van Roy, and Franki Speleman. 2006. “Genome Wide Measurement of DNA Copy Number Changes in Neuroblastoma: Dissecting Amplicons and Mapping Losses, Gains and Breakpoints.” Cytogenetic and Genome Research 115 (3-4): 273–282.
APA
Michels, E., Vandesompele, J., Hoebeeck, J., Menten, B., De Preter, K., Laureys, G., Van Roy, N., et al. (2006). Genome wide measurement of DNA copy number changes in neuroblastoma: dissecting amplicons and mapping losses, gains and breakpoints. CYTOGENETIC AND GENOME RESEARCH, 115(3-4), 273–282.
Vancouver
1.
Michels E, Vandesompele J, Hoebeeck J, Menten B, De Preter K, Laureys G, et al. Genome wide measurement of DNA copy number changes in neuroblastoma: dissecting amplicons and mapping losses, gains and breakpoints. CYTOGENETIC AND GENOME RESEARCH. 2006;115(3-4):273–82.
MLA
Michels, Evi, Jo Vandesompele, Jasmien Hoebeeck, et al. “Genome Wide Measurement of DNA Copy Number Changes in Neuroblastoma: Dissecting Amplicons and Mapping Losses, Gains and Breakpoints.” CYTOGENETIC AND GENOME RESEARCH 115.3-4 (2006): 273–282. Print.
@article{413438,
  abstract     = {In the past few years high throughput methods for assessment of DNA copy number alterations have witnessed rapid progress. Both 'in house' developed BAC, cDNA, oligonucleotide and commercial arrays are now available and widely applied in the study of the human genome, particularly in the context of disease. Cancer cells are known to exhibit DNA losses, gains and amplifications affecting tumor suppressor genes and proto-oncogenes. Moreover, these patterns of genomic imbalances may be associated with particular tumor types or subtypes and may have prognostic value. Here we summarize recent array CGH findings in neuroblastoma, a pediatric tumor of the sympathetic nervous system. A total of 176 primary tumors and 53 cell lines have been analyzed on different platforms. Through these studies the genomic content and boundaries of deletions, gains and amplifications were characterized with unprecedented accuracy. Furthermore, in conjunction with cytogenetic findings, array CGH allows the mapping of breakpoints of unbalanced translocations at a very high resolution.},
  author       = {Michels, Evi and Vandesompele, Jo and Hoebeeck, Jasmien and Menten, Bj{\"o}rn and De Preter, Katleen and Laureys, Genevieve and Van Roy, Nadine and Speleman, Franki},
  issn         = {1424-8581},
  journal      = {CYTOGENETIC AND GENOME RESEARCH},
  keyword      = {HYBRIDIZATION CGH ANALYSIS,HIGH-RESOLUTION ANALYSIS,ACUTE MYELOID LEUKEMIAS,TUMOR-SUPPRESSOR GENE,HUMAN NEURO-BLASTOMA,COMMON ALLELIC LOSS,CELL-LINES,ARRAY-CGH,N-MYC,CYTOGENETIC ANALYSIS},
  language     = {eng},
  number       = {3-4},
  pages        = {273--282},
  title        = {Genome wide measurement of DNA copy number changes in neuroblastoma: dissecting amplicons and mapping losses, gains and breakpoints},
  url          = {http://dx.doi.org/10.1159/000095924},
  volume       = {115},
  year         = {2006},
}

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