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The role of scaffold architecture and composition on the bone formation by adipose-derived stem cells

Heidi Declercq (UGent) , Tim Desmet (UGent) , Peter Dubruel (UGent) and Maria Cornelissen (UGent)
(2014) TISSUE ENGINEERING PART A. 20(1-2). p.434-444
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Abstract
Scaffold architecture and composition are crucial parameters determining the initial cell spatial distribution and consequently bone tissue formation. Three-dimensional poly-ε-caprolactone (PCL) scaffolds with a 0/90° lay-down pattern were plotted and subjected to 1) an oxygen plasma (PCL O) or 2) a post-argon plasma modification with gelatine and fibronectin (PCL Fn). These scaffolds with an open pore structure were compared with more compact scaffolds fabricated by conventional processing techniques: oxidized polylactic acid (LA O) and collagen (COL) scaffolds. Human adipose tissue derived stem cell/scaffold interaction was studied. The study revealed that the biomimetic surface modification of plotted scaffolds did not increase the seeding efficiency. The proliferation and colonization was superior for PCL Fn in comparison with PCL O. The plotted PCL Fn was completely colonized throughout the scaffold whereas conventional scaffolds only at the edge. Protein-based scaffolds (PCL Fn and COL) enhanced the differentiation, although plotted scaffolds showed a delay in their differentiation compared with compact scaffolds. In conclusion, protein modification of plotted PCL scaffolds enhances uniform tissue formation but shows a delayed differentiation in comparison with compact scaffolds. The present study demonstrates that biomimetic PCL scaffolds could serve as a guiding template to obtain a uniform bone tissue formation in vivo.
Keywords
TISSUE FORMATION, MARROW STROMAL CELLS, SURFACE MODIFICATION, COLLAGEN, GRAFTS

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Chicago
Declercq, Heidi, Tim Desmet, Peter Dubruel, and Maria Cornelissen. 2014. “The Role of Scaffold Architecture and Composition on the Bone Formation by Adipose-derived Stem Cells.” Tissue Engineering Part A 20 (1-2): 434–444.
APA
Declercq, H., Desmet, T., Dubruel, P., & Cornelissen, M. (2014). The role of scaffold architecture and composition on the bone formation by adipose-derived stem cells. TISSUE ENGINEERING PART A, 20(1-2), 434–444.
Vancouver
1.
Declercq H, Desmet T, Dubruel P, Cornelissen M. The role of scaffold architecture and composition on the bone formation by adipose-derived stem cells. TISSUE ENGINEERING PART A. 2014;20(1-2):434–44.
MLA
Declercq, Heidi, Tim Desmet, Peter Dubruel, et al. “The Role of Scaffold Architecture and Composition on the Bone Formation by Adipose-derived Stem Cells.” TISSUE ENGINEERING PART A 20.1-2 (2014): 434–444. Print.
@article{4127918,
  abstract     = {Scaffold architecture and composition are crucial parameters determining the initial cell spatial distribution and consequently bone tissue formation. Three-dimensional poly-\ensuremath{\epsilon}-caprolactone (PCL) scaffolds with a 0/90{\textdegree} lay-down pattern were plotted and subjected to 1) an oxygen plasma (PCL O) or 2) a post-argon plasma modification with gelatine and fibronectin (PCL Fn). These scaffolds with an open pore structure were compared with more compact scaffolds fabricated by conventional processing techniques: oxidized polylactic acid (LA O) and collagen (COL) scaffolds. Human adipose tissue derived stem cell/scaffold interaction was studied. The study revealed that the biomimetic surface modification of plotted scaffolds did not increase the seeding efficiency. The proliferation and colonization was superior for PCL Fn in comparison with PCL O. The plotted PCL Fn was completely colonized throughout the scaffold whereas conventional scaffolds only at the edge. Protein-based scaffolds (PCL Fn and COL) enhanced the differentiation, although plotted scaffolds showed a delay in their differentiation compared with compact scaffolds. In conclusion, protein modification of plotted PCL scaffolds enhances uniform tissue formation but shows a delayed differentiation in comparison with compact scaffolds. The present study demonstrates that biomimetic PCL scaffolds could serve as a guiding template to obtain a uniform bone tissue formation in vivo.},
  author       = {Declercq, Heidi and Desmet, Tim and Dubruel, Peter and Cornelissen, Maria},
  issn         = {1937-3341},
  journal      = {TISSUE ENGINEERING PART A},
  language     = {eng},
  number       = {1-2},
  pages        = {434--444},
  title        = {The role of scaffold architecture and composition on the bone formation by adipose-derived stem cells},
  url          = {http://dx.doi.org/10.1089/ten.TEA.2013.0179},
  volume       = {20},
  year         = {2014},
}

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