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MTV1 and MTV4 encode plant-specific ENTH and ARF GAP proteins that mediate clathrin-dependent trafficking of vacuolar cargo from the trans-Golgi network

Michael Sauer, M Otilia Delgadillo, Jan Zouhar, Gregory D Reynolds, Janice G Pennington, Liwen Jiang, Sarah J Liljegren, York-Dieter Stierhof, Geert De Jaeger UGent, Marisa S Otegui, et al. (2013) PLANT CELL. 25(6). p.2217-2235
abstract
Many soluble proteins transit through the trans-Golgi network (TGN) and the prevacuolar compartment (PVC) en route to the vacuole, but our mechanistic understanding of this vectorial trafficking step in plants is limited. In particular, it is unknown whether clathrin-coated vesicles (CCVs) participate in this transport step. Through a screen for modified transport to the vacuole (mtv) mutants that secrete the vacuolar protein VAC2, we identified MTV1, which encodes an EPSIN N-TERMINAL HOMOLOGY protein, and MTV4, which encodes the ADP ribosylation factor GTPase-activating protein NEVERSHED/AGD5. MTV1 and NEV/AGD5 have overlapping expression patterns and interact genetically to transport vacuolar cargo and promote plant growth, but they have no apparent roles in protein secretion or endocytosis. MTV1 and NEV/AGD5 colocalize with clathrin at the TGN and are incorporated into CCVs. Importantly, mtv1 nev/agd5 double mutants show altered subcellular distribution of CCV cargo exported from the TGN. Moreover, MTV1 binds clathrin in vitro, and NEV/AGD5 associates in vivo with clathrin, directly linking these proteins to CCV formation. These results indicate that MTV1 and NEV/AGD5 are key effectors for CCV-mediated trafficking of vacuolar proteins from the TGN to the PVC in plants.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
INTRACELLULAR TRAFFICKING, PREVACUOLAR COMPARTMENTS, COATED VESICLES, MULTIVESICULAR BODIES, ENDOPLASMIC-RETICULUM, SORTING RECEPTOR, ARABIDOPSIS THALIANA, SEED STORAGE PROTEINS, TANDEM AFFINITY PURIFICATION, GTPASE-ACTIVATING PROTEIN
journal title
PLANT CELL
Plant Cell
volume
25
issue
6
pages
2217 - 2235
Web of Science type
Article
Web of Science id
000322371500025
JCR category
PLANT SCIENCES
JCR impact factor
9.575 (2013)
JCR rank
4/199 (2013)
JCR quartile
1 (2013)
ISSN
1040-4651
DOI
10.1105/tpc.113.111724
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4127391
handle
http://hdl.handle.net/1854/LU-4127391
date created
2013-09-05 11:40:16
date last changed
2016-12-19 15:43:03
@article{4127391,
  abstract     = {Many soluble proteins transit through the trans-Golgi network (TGN) and the prevacuolar compartment (PVC) en route to the vacuole, but our mechanistic understanding of this vectorial trafficking step in plants is limited. In particular, it is unknown whether clathrin-coated vesicles (CCVs) participate in this transport step. Through a screen for modified transport to the vacuole (mtv) mutants that secrete the vacuolar protein VAC2, we identified MTV1, which encodes an EPSIN N-TERMINAL HOMOLOGY protein, and MTV4, which encodes the ADP ribosylation factor GTPase-activating protein NEVERSHED/AGD5. MTV1 and NEV/AGD5 have overlapping expression patterns and interact genetically to transport vacuolar cargo and promote plant growth, but they have no apparent roles in protein secretion or endocytosis. MTV1 and NEV/AGD5 colocalize with clathrin at the TGN and are incorporated into CCVs. Importantly, mtv1 nev/agd5 double mutants show altered subcellular distribution of CCV cargo exported from the TGN. Moreover, MTV1 binds clathrin in vitro, and NEV/AGD5 associates in vivo with clathrin, directly linking these proteins to CCV formation. These results indicate that MTV1 and NEV/AGD5 are key effectors for CCV-mediated trafficking of vacuolar proteins from the TGN to the PVC in plants.},
  author       = {Sauer, Michael and Delgadillo, M Otilia and Zouhar, Jan and Reynolds, Gregory D and Pennington, Janice G and Jiang, Liwen and Liljegren, Sarah J and Stierhof, York-Dieter and De Jaeger, Geert and Otegui, Marisa S and Bednarek, Sebastian Y and Rojo, Enrique},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {INTRACELLULAR TRAFFICKING,PREVACUOLAR COMPARTMENTS,COATED VESICLES,MULTIVESICULAR BODIES,ENDOPLASMIC-RETICULUM,SORTING RECEPTOR,ARABIDOPSIS THALIANA,SEED STORAGE PROTEINS,TANDEM AFFINITY PURIFICATION,GTPASE-ACTIVATING PROTEIN},
  language     = {eng},
  number       = {6},
  pages        = {2217--2235},
  title        = {MTV1 and MTV4 encode plant-specific ENTH and ARF GAP proteins that mediate clathrin-dependent trafficking of vacuolar cargo from the trans-Golgi network},
  url          = {http://dx.doi.org/10.1105/tpc.113.111724},
  volume       = {25},
  year         = {2013},
}

Chicago
Sauer, Michael, M Otilia Delgadillo, Jan Zouhar, Gregory D Reynolds, Janice G Pennington, Liwen Jiang, Sarah J Liljegren, et al. 2013. “MTV1 and MTV4 Encode Plant-specific ENTH and ARF GAP Proteins That Mediate Clathrin-dependent Trafficking of Vacuolar Cargo from the trans-Golgi Network.” Plant Cell 25 (6): 2217–2235.
APA
Sauer, M., Delgadillo, M. O., Zouhar, J., Reynolds, G. D., Pennington, J. G., Jiang, L., Liljegren, S. J., et al. (2013). MTV1 and MTV4 encode plant-specific ENTH and ARF GAP proteins that mediate clathrin-dependent trafficking of vacuolar cargo from the trans-Golgi network. PLANT CELL, 25(6), 2217–2235.
Vancouver
1.
Sauer M, Delgadillo MO, Zouhar J, Reynolds GD, Pennington JG, Jiang L, et al. MTV1 and MTV4 encode plant-specific ENTH and ARF GAP proteins that mediate clathrin-dependent trafficking of vacuolar cargo from the trans-Golgi network. PLANT CELL. 2013;25(6):2217–35.
MLA
Sauer, Michael, M Otilia Delgadillo, Jan Zouhar, et al. “MTV1 and MTV4 Encode Plant-specific ENTH and ARF GAP Proteins That Mediate Clathrin-dependent Trafficking of Vacuolar Cargo from the trans-Golgi Network.” PLANT CELL 25.6 (2013): 2217–2235. Print.