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MTV1 and MTV4 encode plant-specific ENTH and ARF GAP proteins that mediate clathrin-dependent trafficking of vacuolar cargo from the trans-Golgi network

(2013) PLANT CELL. 25(6). p.2217-2235
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Abstract
Many soluble proteins transit through the trans-Golgi network (TGN) and the prevacuolar compartment (PVC) en route to the vacuole, but our mechanistic understanding of this vectorial trafficking step in plants is limited. In particular, it is unknown whether clathrin-coated vesicles (CCVs) participate in this transport step. Through a screen for modified transport to the vacuole (mtv) mutants that secrete the vacuolar protein VAC2, we identified MTV1, which encodes an EPSIN N-TERMINAL HOMOLOGY protein, and MTV4, which encodes the ADP ribosylation factor GTPase-activating protein NEVERSHED/AGD5. MTV1 and NEV/AGD5 have overlapping expression patterns and interact genetically to transport vacuolar cargo and promote plant growth, but they have no apparent roles in protein secretion or endocytosis. MTV1 and NEV/AGD5 colocalize with clathrin at the TGN and are incorporated into CCVs. Importantly, mtv1 nev/agd5 double mutants show altered subcellular distribution of CCV cargo exported from the TGN. Moreover, MTV1 binds clathrin in vitro, and NEV/AGD5 associates in vivo with clathrin, directly linking these proteins to CCV formation. These results indicate that MTV1 and NEV/AGD5 are key effectors for CCV-mediated trafficking of vacuolar proteins from the TGN to the PVC in plants.
Keywords
INTRACELLULAR TRAFFICKING, PREVACUOLAR COMPARTMENTS, COATED VESICLES, MULTIVESICULAR BODIES, ENDOPLASMIC-RETICULUM, SORTING RECEPTOR, ARABIDOPSIS THALIANA, SEED STORAGE PROTEINS, TANDEM AFFINITY PURIFICATION, GTPASE-ACTIVATING PROTEIN

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Citation

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Chicago
Sauer, Michael, M Otilia Delgadillo, Jan Zouhar, Gregory D Reynolds, Janice G Pennington, Liwen Jiang, Sarah J Liljegren, et al. 2013. “MTV1 and MTV4 Encode Plant-specific ENTH and ARF GAP Proteins That Mediate Clathrin-dependent Trafficking of Vacuolar Cargo from the trans-Golgi Network.” Plant Cell 25 (6): 2217–2235.
APA
Sauer, M., Delgadillo, M. O., Zouhar, J., Reynolds, G. D., Pennington, J. G., Jiang, L., Liljegren, S. J., et al. (2013). MTV1 and MTV4 encode plant-specific ENTH and ARF GAP proteins that mediate clathrin-dependent trafficking of vacuolar cargo from the trans-Golgi network. PLANT CELL, 25(6), 2217–2235.
Vancouver
1.
Sauer M, Delgadillo MO, Zouhar J, Reynolds GD, Pennington JG, Jiang L, et al. MTV1 and MTV4 encode plant-specific ENTH and ARF GAP proteins that mediate clathrin-dependent trafficking of vacuolar cargo from the trans-Golgi network. PLANT CELL. 2013;25(6):2217–35.
MLA
Sauer, Michael, M Otilia Delgadillo, Jan Zouhar, et al. “MTV1 and MTV4 Encode Plant-specific ENTH and ARF GAP Proteins That Mediate Clathrin-dependent Trafficking of Vacuolar Cargo from the trans-Golgi Network.” PLANT CELL 25.6 (2013): 2217–2235. Print.
@article{4127391,
  abstract     = {Many soluble proteins transit through the trans-Golgi network (TGN) and the prevacuolar compartment (PVC) en route to the vacuole, but our mechanistic understanding of this vectorial trafficking step in plants is limited. In particular, it is unknown whether clathrin-coated vesicles (CCVs) participate in this transport step. Through a screen for modified transport to the vacuole (mtv) mutants that secrete the vacuolar protein VAC2, we identified MTV1, which encodes an EPSIN N-TERMINAL HOMOLOGY protein, and MTV4, which encodes the ADP ribosylation factor GTPase-activating protein NEVERSHED/AGD5. MTV1 and NEV/AGD5 have overlapping expression patterns and interact genetically to transport vacuolar cargo and promote plant growth, but they have no apparent roles in protein secretion or endocytosis. MTV1 and NEV/AGD5 colocalize with clathrin at the TGN and are incorporated into CCVs. Importantly, mtv1 nev/agd5 double mutants show altered subcellular distribution of CCV cargo exported from the TGN. Moreover, MTV1 binds clathrin in vitro, and NEV/AGD5 associates in vivo with clathrin, directly linking these proteins to CCV formation. These results indicate that MTV1 and NEV/AGD5 are key effectors for CCV-mediated trafficking of vacuolar proteins from the TGN to the PVC in plants.},
  author       = {Sauer, Michael and Delgadillo, M Otilia and Zouhar, Jan and Reynolds, Gregory D and Pennington, Janice G and Jiang, Liwen and Liljegren, Sarah J and Stierhof, York-Dieter and De Jaeger, Geert and Otegui, Marisa S and Bednarek, Sebastian Y and Rojo, Enrique},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {INTRACELLULAR TRAFFICKING,PREVACUOLAR COMPARTMENTS,COATED VESICLES,MULTIVESICULAR BODIES,ENDOPLASMIC-RETICULUM,SORTING RECEPTOR,ARABIDOPSIS THALIANA,SEED STORAGE PROTEINS,TANDEM AFFINITY PURIFICATION,GTPASE-ACTIVATING PROTEIN},
  language     = {eng},
  number       = {6},
  pages        = {2217--2235},
  title        = {MTV1 and MTV4 encode plant-specific ENTH and ARF GAP proteins that mediate clathrin-dependent trafficking of vacuolar cargo from the trans-Golgi network},
  url          = {http://dx.doi.org/10.1105/tpc.113.111724},
  volume       = {25},
  year         = {2013},
}

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