Advanced search
1 file | 318.54 KB

Characterization of strains of Weissella fabalis sp. nov. and Fructobacillus tropaeoli from spontaneous cocoa bean fermentations

Author
Organization
Abstract
Six facultatively anaerobic, non-motile lactic acid bacteria were isolated from spontaneous cocoa bean fermentations carried out in Brazil, Ecuador and Malaysia. Phylogenetic analysis revealed that one of these strains, designated M75T, isolated from a Brazilian cocoa bean fermentation, had the highest 16S rRNA gene sequence similarity towards Weissella fabaria LMG 24289T (97.7 %), W. ghanensis LMG 24286T (93.3 %) and W. beninensis LMG 25373T (93.4 %). The remaining lactic acid bacteria isolates, represented by strain M622, showed the highest 16S rRNA gene sequence similarity towards the type strain of Fructobacillus tropaeoli (99.9 %), a recently described species isolated from a flower in South Africa. pheS gene sequence analysis indicated that the former strain represented a novel species, whereas pheS, rpoA and atpA gene sequence analysis indicated that the remaining five strains belonged to F. tropaeoli; these results were confirmed by DNA–DNA hybridization experiments towards their respective nearest phylogenetic neighbours. Additionally, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry proved successful for the identification of species of the genera Weissella and Fructobacillus and for the recognition of the novel species. We propose to classify strain M75T ( = LMG 26217T  = CCUG 61472T) as the type strain of the novel species Weissella fabalis sp. nov.
Keywords
ARB, DNA, HYBRIDIZATION, IDENTIFICATION, LEUCONOSTOC, GENUS WEISSELLA, SEQUENCE-ANALYSIS, LACTIC-ACID BACTERIUM, SPECIES-DIVERSITY

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 318.54 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Snauwaert, Isabel, Zoi Papalexandratou, Luc De Vuyst, and Peter Vandamme. 2013. “Characterization of Strains of Weissella Fabalis Sp. Nov. and Fructobacillus Tropaeoli from Spontaneous Cocoa Bean Fermentations.” International Journal of Systematic and Evolutionary Microbiology 63 (5): 1709–1716.
APA
Snauwaert, I., Papalexandratou, Z., De Vuyst, L., & Vandamme, P. (2013). Characterization of strains of Weissella fabalis sp. nov. and Fructobacillus tropaeoli from spontaneous cocoa bean fermentations. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 63(5), 1709–1716.
Vancouver
1.
Snauwaert I, Papalexandratou Z, De Vuyst L, Vandamme P. Characterization of strains of Weissella fabalis sp. nov. and Fructobacillus tropaeoli from spontaneous cocoa bean fermentations. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2013;63(5):1709–16.
MLA
Snauwaert, Isabel, Zoi Papalexandratou, Luc De Vuyst, et al. “Characterization of Strains of Weissella Fabalis Sp. Nov. and Fructobacillus Tropaeoli from Spontaneous Cocoa Bean Fermentations.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 63.5 (2013): 1709–1716. Print.
@article{4127382,
  abstract     = {Six facultatively anaerobic, non-motile lactic acid bacteria were isolated from spontaneous cocoa bean fermentations carried out in Brazil, Ecuador and Malaysia. Phylogenetic analysis revealed that one of these strains, designated M75T, isolated from a Brazilian cocoa bean fermentation, had the highest 16S rRNA gene sequence similarity towards Weissella fabaria LMG 24289T (97.7\ensuremath{\mkern1mu}\%), W. ghanensis LMG 24286T (93.3\ensuremath{\mkern1mu}\%) and W. beninensis LMG 25373T (93.4\ensuremath{\mkern1mu}\%). The remaining lactic acid bacteria isolates, represented by strain M622, showed the highest 16S rRNA gene sequence similarity towards the type strain of Fructobacillus tropaeoli (99.9\ensuremath{\mkern1mu}\%), a recently described species isolated from a flower in South Africa. pheS gene sequence analysis indicated that the former strain represented a novel species, whereas pheS, rpoA and atpA gene sequence analysis indicated that the remaining five strains belonged to F. tropaeoli; these results were confirmed by DNA--DNA hybridization experiments towards their respective nearest phylogenetic neighbours. Additionally, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry proved successful for the identification of species of the genera Weissella and Fructobacillus and for the recognition of the novel species. We propose to classify strain M75T (\ensuremath{\mkern1mu}=\ensuremath{\mkern1mu}LMG 26217T \ensuremath{\mkern1mu}=\ensuremath{\mkern1mu}CCUG 61472T) as the type strain of the novel species Weissella fabalis sp. nov.},
  author       = {Snauwaert, Isabel and Papalexandratou, Zoi and De Vuyst, Luc and Vandamme, Peter},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  language     = {eng},
  number       = {5},
  pages        = {1709--1716},
  title        = {Characterization of strains of Weissella fabalis sp. nov. and Fructobacillus tropaeoli from spontaneous cocoa bean fermentations},
  url          = {http://dx.doi.org/10.1099/ijs.0.040311-0},
  volume       = {63},
  year         = {2013},
}

Altmetric
View in Altmetric
Web of Science
Times cited: