Advanced search
1 file | 2.37 MB Add to list

Measuring the intravitreal mobility of nanomedicines with single-particle tracking microscopy

(2013) NANOMEDICINE. 8(12). p.1955-1968
Author
Organization
Project
Center for nano- and biophotonics (NB-Photonics)
Abstract
Aim: To develop a robust assay to evaluate and compare the intravitreal mobility of nanoparticles in the intact vitreous body. Materials & methods: Excised bovine eyes were prepared to preserve the fragile structure of the vitreous humor, while permitting high-resolution fluorescence microscopy and single-particle tracking analysis of intravitreally injected nanoparticles. This assay was validated by analyzing polystyrene beads and further employed to evaluate gene nanomedicines composed of poly(amido amine)s and plasmid DNA. Results: The assay was able to distinguish immobilized cationic nanoparticles from mobile PEGylated nanoparticles. PEGylation of the polyplexes resulted in a drastic improvement of their mobility. Conclusion: An ex vivo eye model is presented for studying nanoparticle mobility in intact vitreous humor by single-particle tracking microscopy. These results give important guidelines for developing gene- and drug-delivery nanomedicines that are compatible with intravitreal administration.
Keywords
NANOPARTICLES, INJECTION, THERAPY, MOUSE RETINA, VIRAL VECTORS, VITREOUS-HUMOR, DIFFUSIVE TRANSPORT, DRUG-DELIVERY, PIGMENT EPITHELIAL-CELLS, OCULAR GENE-TRANSFER, single-particle tracking, polyplex, ocular gene therapy, nanoparticle, nanomedicine, intravitreal injection, diffusion

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 2.37 MB

Citation

Please use this url to cite or link to this publication:

MLA
Martens, Thomas, Dries Vercauteren, Katrien Forier, et al. “Measuring the Intravitreal Mobility of Nanomedicines with Single-particle Tracking Microscopy.” NANOMEDICINE 8.12 (2013): 1955–1968. Print.
APA
Martens, T., Vercauteren, D., Forier, K., Deschout, H., Remaut, K., Paesen, R., Ameloot, M., et al. (2013). Measuring the intravitreal mobility of nanomedicines with single-particle tracking microscopy. NANOMEDICINE, 8(12), 1955–1968.
Chicago author-date
Martens, Thomas, Dries Vercauteren, Katrien Forier, Hendrik Deschout, Katrien Remaut, Rik Paesen, Marcel Ameloot, et al. 2013. “Measuring the Intravitreal Mobility of Nanomedicines with Single-particle Tracking Microscopy.” Nanomedicine 8 (12): 1955–1968.
Chicago author-date (all authors)
Martens, Thomas, Dries Vercauteren, Katrien Forier, Hendrik Deschout, Katrien Remaut, Rik Paesen, Marcel Ameloot, Johan FJ Engbersen, Jo Demeester, Stefaan De Smedt, and Kevin Braeckmans. 2013. “Measuring the Intravitreal Mobility of Nanomedicines with Single-particle Tracking Microscopy.” Nanomedicine 8 (12): 1955–1968.
Vancouver
1.
Martens T, Vercauteren D, Forier K, Deschout H, Remaut K, Paesen R, et al. Measuring the intravitreal mobility of nanomedicines with single-particle tracking microscopy. NANOMEDICINE. 2013;8(12):1955–68.
IEEE
[1]
T. Martens et al., “Measuring the intravitreal mobility of nanomedicines with single-particle tracking microscopy,” NANOMEDICINE, vol. 8, no. 12, pp. 1955–1968, 2013.
@article{4125645,
  abstract     = {Aim: To develop a robust assay to evaluate and compare the intravitreal mobility of nanoparticles in the intact vitreous body.
Materials & methods: Excised bovine eyes were prepared to preserve the fragile structure of the vitreous humor, while permitting high-resolution fluorescence microscopy and single-particle tracking analysis of intravitreally injected nanoparticles. This assay was validated by analyzing polystyrene beads and further employed to evaluate gene nanomedicines composed of poly(amido amine)s and plasmid DNA.
Results: The assay was able to distinguish immobilized cationic nanoparticles from mobile PEGylated nanoparticles. PEGylation of the polyplexes resulted in a drastic improvement of their mobility.
Conclusion: An ex vivo eye model is presented for studying nanoparticle mobility in intact vitreous humor by single-particle tracking microscopy. These results give important guidelines for developing gene- and drug-delivery nanomedicines that are compatible with intravitreal administration.},
  author       = {Martens, Thomas and Vercauteren, Dries and Forier, Katrien and Deschout, Hendrik and Remaut, Katrien and Paesen, Rik and Ameloot, Marcel and Engbersen, Johan FJ and Demeester, Jo and De Smedt, Stefaan and Braeckmans, Kevin},
  issn         = {1743-5889},
  journal      = {NANOMEDICINE},
  keywords     = {NANOPARTICLES,INJECTION,THERAPY,MOUSE RETINA,VIRAL VECTORS,VITREOUS-HUMOR,DIFFUSIVE TRANSPORT,DRUG-DELIVERY,PIGMENT EPITHELIAL-CELLS,OCULAR GENE-TRANSFER,single-particle tracking,polyplex,ocular gene therapy,nanoparticle,nanomedicine,intravitreal injection,diffusion},
  language     = {eng},
  number       = {12},
  pages        = {1955--1968},
  title        = {Measuring the intravitreal mobility of nanomedicines with single-particle tracking microscopy},
  url          = {http://dx.doi.org/10.2217/NNM.12.202},
  volume       = {8},
  year         = {2013},
}

Altmetric
View in Altmetric
Web of Science
Times cited: