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The ROSA26-iPSC mouse: a conditional, inducible, and exchangeable resource for studying cellular (de)differentiation

Lieven Haenebalcke, Steven Goossens UGent, Pieterjan Dierickx UGent, Sona Bartunkova UGent, Jinke D'Hont UGent, Katharina Haigh UGent, Tino Hochepied UGent, Dagmar Wirth, Andras Nagy and Jody Haigh (2013) CELL REPORTS. 3(2). p.335-341
abstract
Control of cellular (de)differentiation in a temporal, cell-specific, and exchangeable manner is of paramount importance in the field of reprogramming. Here, we have generated and characterized a mouse strain that allows iPSC generation through the Cre/loxP conditional and doxycycline/rtTA-controlled inducible expression of the OSKM reprogramming factors entirely from within the ROSA26 locus. After reprogramming, these factors can be replaced by genes of interest-for example, to enhance lineage-directed differentiation-with the use of a trap-coupled RMCE reaction. We show that, similar to ESCs, Dox-controlled expression of the cardiac transcriptional regulator Mesp1 together with Wnt inhibition enhances the generation of functional cardiomyocytes upon in vitro differentiation of such RMCE-retargeted iPSCs. This ROSA26-iPSC mouse model is therefore an excellent tool for studying both cellular reprogramming and lineage-directed differentiation factors from the same locus and will greatly facilitate the identification and ease of functional characterization of the genetic/epigenetic determinants involved in these complex processes.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
RECOMBINATION, INDUCTION, LOCUS, MODEL, TIME, MESP1
journal title
CELL REPORTS
Cell Reports
volume
3
issue
2
pages
335 - 341
Web of Science type
Article
Web of Science id
000321895200009
JCR category
CELL BIOLOGY
JCR impact factor
7.207 (2013)
JCR rank
32/185 (2013)
JCR quartile
1 (2013)
ISSN
2211-1247
DOI
10.1016/j.celrep.2013.01.016
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4118026
handle
http://hdl.handle.net/1854/LU-4118026
date created
2013-08-21 10:24:15
date last changed
2016-12-19 15:43:39
@article{4118026,
  abstract     = {Control of cellular (de)differentiation in a temporal, cell-specific, and exchangeable manner is of paramount importance in the field of reprogramming. Here, we have generated and characterized a mouse strain that allows iPSC generation through the Cre/loxP conditional and doxycycline/rtTA-controlled inducible expression of the OSKM reprogramming factors entirely from within the ROSA26 locus. After reprogramming, these factors can be replaced by genes of interest-for example, to enhance lineage-directed differentiation-with the use of a trap-coupled RMCE reaction. We show that, similar to ESCs, Dox-controlled expression of the cardiac transcriptional regulator Mesp1 together with Wnt inhibition enhances the generation of functional cardiomyocytes upon in vitro differentiation of such RMCE-retargeted iPSCs. This ROSA26-iPSC mouse model is therefore an excellent tool for studying both cellular reprogramming and lineage-directed differentiation factors from the same locus and will greatly facilitate the identification and ease of functional characterization of the genetic/epigenetic determinants involved in these complex processes.},
  author       = {Haenebalcke, Lieven and Goossens, Steven and Dierickx, Pieterjan and Bartunkova, Sona and D'Hont, Jinke and Haigh, Katharina and Hochepied, Tino and Wirth, Dagmar and Nagy, Andras and Haigh, Jody},
  issn         = {2211-1247},
  journal      = {CELL REPORTS},
  keyword      = {RECOMBINATION,INDUCTION,LOCUS,MODEL,TIME,MESP1},
  language     = {eng},
  number       = {2},
  pages        = {335--341},
  title        = {The ROSA26-iPSC mouse: a conditional, inducible, and exchangeable resource for studying cellular (de)differentiation},
  url          = {http://dx.doi.org/10.1016/j.celrep.2013.01.016},
  volume       = {3},
  year         = {2013},
}

Chicago
Haenebalcke, Lieven, Steven Goossens, Pieterjan Dierickx, Sona Bartunkova, Jinke D’Hont, Katharina Haigh, Tino Hochepied, Dagmar Wirth, Andras Nagy, and Jody Haigh. 2013. “The ROSA26-iPSC Mouse: a Conditional, Inducible, and Exchangeable Resource for Studying Cellular (de)differentiation.” Cell Reports 3 (2): 335–341.
APA
Haenebalcke, L., Goossens, S., Dierickx, P., Bartunkova, S., D’Hont, J., Haigh, K., Hochepied, T., et al. (2013). The ROSA26-iPSC mouse: a conditional, inducible, and exchangeable resource for studying cellular (de)differentiation. CELL REPORTS, 3(2), 335–341.
Vancouver
1.
Haenebalcke L, Goossens S, Dierickx P, Bartunkova S, D’Hont J, Haigh K, et al. The ROSA26-iPSC mouse: a conditional, inducible, and exchangeable resource for studying cellular (de)differentiation. CELL REPORTS. 2013;3(2):335–41.
MLA
Haenebalcke, Lieven, Steven Goossens, Pieterjan Dierickx, et al. “The ROSA26-iPSC Mouse: a Conditional, Inducible, and Exchangeable Resource for Studying Cellular (de)differentiation.” CELL REPORTS 3.2 (2013): 335–341. Print.