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The ROSA26-iPSC mouse : a conditional, inducible, and exchangeable resource for studying cellular (de)differentiation

Lieven Haenebalcke (UGent) , Steven Goossens (UGent) , Pieterjan Dierickx (UGent) , Sona Bartunkova (UGent) , Jinke D'Hont (UGent) , Katharina Haigh (UGent) , Tino Hochepied (UGent) , Dagmar Wirth, Andras Nagy and Jody Haigh (UGent)
(2013) CELL REPORTS. 3(2). p.335-341
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Abstract
Control of cellular (de)differentiation in a temporal, cell-specific, and exchangeable manner is of paramount importance in the field of reprogramming. Here, we have generated and characterized a mouse strain that allows iPSC generation through the Cre/loxP conditional and doxycycline/rtTA-controlled inducible expression of the OSKM reprogramming factors entirely from within the ROSA26 locus. After reprogramming, these factors can be replaced by genes of interest-for example, to enhance lineage-directed differentiation-with the use of a trap-coupled RMCE reaction. We show that, similar to ESCs, Dox-controlled expression of the cardiac transcriptional regulator Mesp1 together with Wnt inhibition enhances the generation of functional cardiomyocytes upon in vitro differentiation of such RMCE-retargeted iPSCs. This ROSA26-iPSC mouse model is therefore an excellent tool for studying both cellular reprogramming and lineage-directed differentiation factors from the same locus and will greatly facilitate the identification and ease of functional characterization of the genetic/epigenetic determinants involved in these complex processes.
Keywords
RECOMBINATION, INDUCTION, LOCUS, MODEL, TIME, MESP1

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MLA
Haenebalcke, Lieven, et al. “The ROSA26-IPSC Mouse : A Conditional, Inducible, and Exchangeable Resource for Studying Cellular (de)Differentiation.” CELL REPORTS, vol. 3, no. 2, 2013, pp. 335–41, doi:10.1016/j.celrep.2013.01.016.
APA
Haenebalcke, L., Goossens, S., Dierickx, P., Bartunkova, S., D’Hont, J., Haigh, K., … Haigh, J. (2013). The ROSA26-iPSC mouse : a conditional, inducible, and exchangeable resource for studying cellular (de)differentiation. CELL REPORTS, 3(2), 335–341. https://doi.org/10.1016/j.celrep.2013.01.016
Chicago author-date
Haenebalcke, Lieven, Steven Goossens, Pieterjan Dierickx, Sona Bartunkova, Jinke D’Hont, Katharina Haigh, Tino Hochepied, Dagmar Wirth, Andras Nagy, and Jody Haigh. 2013. “The ROSA26-IPSC Mouse : A Conditional, Inducible, and Exchangeable Resource for Studying Cellular (de)Differentiation.” CELL REPORTS 3 (2): 335–41. https://doi.org/10.1016/j.celrep.2013.01.016.
Chicago author-date (all authors)
Haenebalcke, Lieven, Steven Goossens, Pieterjan Dierickx, Sona Bartunkova, Jinke D’Hont, Katharina Haigh, Tino Hochepied, Dagmar Wirth, Andras Nagy, and Jody Haigh. 2013. “The ROSA26-IPSC Mouse : A Conditional, Inducible, and Exchangeable Resource for Studying Cellular (de)Differentiation.” CELL REPORTS 3 (2): 335–341. doi:10.1016/j.celrep.2013.01.016.
Vancouver
1.
Haenebalcke L, Goossens S, Dierickx P, Bartunkova S, D’Hont J, Haigh K, et al. The ROSA26-iPSC mouse : a conditional, inducible, and exchangeable resource for studying cellular (de)differentiation. CELL REPORTS. 2013;3(2):335–41.
IEEE
[1]
L. Haenebalcke et al., “The ROSA26-iPSC mouse : a conditional, inducible, and exchangeable resource for studying cellular (de)differentiation,” CELL REPORTS, vol. 3, no. 2, pp. 335–341, 2013.
@article{4118026,
  abstract     = {{Control of cellular (de)differentiation in a temporal, cell-specific, and exchangeable manner is of paramount importance in the field of reprogramming. Here, we have generated and characterized a mouse strain that allows iPSC generation through the Cre/loxP conditional and doxycycline/rtTA-controlled inducible expression of the OSKM reprogramming factors entirely from within the ROSA26 locus. After reprogramming, these factors can be replaced by genes of interest-for example, to enhance lineage-directed differentiation-with the use of a trap-coupled RMCE reaction. We show that, similar to ESCs, Dox-controlled expression of the cardiac transcriptional regulator Mesp1 together with Wnt inhibition enhances the generation of functional cardiomyocytes upon in vitro differentiation of such RMCE-retargeted iPSCs. This ROSA26-iPSC mouse model is therefore an excellent tool for studying both cellular reprogramming and lineage-directed differentiation factors from the same locus and will greatly facilitate the identification and ease of functional characterization of the genetic/epigenetic determinants involved in these complex processes.}},
  author       = {{Haenebalcke, Lieven and Goossens, Steven and Dierickx, Pieterjan and Bartunkova, Sona and D'Hont, Jinke and Haigh, Katharina and Hochepied, Tino and Wirth, Dagmar and Nagy, Andras and Haigh, Jody}},
  issn         = {{2211-1247}},
  journal      = {{CELL REPORTS}},
  keywords     = {{RECOMBINATION,INDUCTION,LOCUS,MODEL,TIME,MESP1}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{335--341}},
  title        = {{The ROSA26-iPSC mouse : a conditional, inducible, and exchangeable resource for studying cellular (de)differentiation}},
  url          = {{http://dx.doi.org/10.1016/j.celrep.2013.01.016}},
  volume       = {{3}},
  year         = {{2013}},
}

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