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The ROSA26-iPSC mouse: a conditional, inducible, and exchangeable resource for studying cellular (de)differentiation

Lieven Haenebalcke (UGent) , Steven Goossens (UGent) , Pieterjan Dierickx (UGent) , Sona Bartunkova (UGent) , Jinke D'Hont (UGent) , Katharina Haigh (UGent) , Tino Hochepied (UGent) , Dagmar Wirth, Andras Nagy and Jody Haigh (UGent)
(2013) CELL REPORTS. 3(2). p.335-341
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Abstract
Control of cellular (de)differentiation in a temporal, cell-specific, and exchangeable manner is of paramount importance in the field of reprogramming. Here, we have generated and characterized a mouse strain that allows iPSC generation through the Cre/loxP conditional and doxycycline/rtTA-controlled inducible expression of the OSKM reprogramming factors entirely from within the ROSA26 locus. After reprogramming, these factors can be replaced by genes of interest-for example, to enhance lineage-directed differentiation-with the use of a trap-coupled RMCE reaction. We show that, similar to ESCs, Dox-controlled expression of the cardiac transcriptional regulator Mesp1 together with Wnt inhibition enhances the generation of functional cardiomyocytes upon in vitro differentiation of such RMCE-retargeted iPSCs. This ROSA26-iPSC mouse model is therefore an excellent tool for studying both cellular reprogramming and lineage-directed differentiation factors from the same locus and will greatly facilitate the identification and ease of functional characterization of the genetic/epigenetic determinants involved in these complex processes.
Keywords
RECOMBINATION, INDUCTION, LOCUS, MODEL, TIME, MESP1

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Chicago
Haenebalcke, Lieven, Steven Goossens, Pieterjan Dierickx, Sona Bartunkova, Jinke D’Hont, Katharina Haigh, Tino Hochepied, Dagmar Wirth, Andras Nagy, and Jody Haigh. 2013. “The ROSA26-iPSC Mouse: a Conditional, Inducible, and Exchangeable Resource for Studying Cellular (de)differentiation.” Cell Reports 3 (2): 335–341.
APA
Haenebalcke, L., Goossens, S., Dierickx, P., Bartunkova, S., D’Hont, J., Haigh, K., Hochepied, T., et al. (2013). The ROSA26-iPSC mouse: a conditional, inducible, and exchangeable resource for studying cellular (de)differentiation. CELL REPORTS, 3(2), 335–341.
Vancouver
1.
Haenebalcke L, Goossens S, Dierickx P, Bartunkova S, D’Hont J, Haigh K, et al. The ROSA26-iPSC mouse: a conditional, inducible, and exchangeable resource for studying cellular (de)differentiation. CELL REPORTS. 2013;3(2):335–41.
MLA
Haenebalcke, Lieven, Steven Goossens, Pieterjan Dierickx, et al. “The ROSA26-iPSC Mouse: a Conditional, Inducible, and Exchangeable Resource for Studying Cellular (de)differentiation.” CELL REPORTS 3.2 (2013): 335–341. Print.
@article{4118026,
  abstract     = {Control of cellular (de)differentiation in a temporal, cell-specific, and exchangeable manner is of paramount importance in the field of reprogramming. Here, we have generated and characterized a mouse strain that allows iPSC generation through the Cre/loxP conditional and doxycycline/rtTA-controlled inducible expression of the OSKM reprogramming factors entirely from within the ROSA26 locus. After reprogramming, these factors can be replaced by genes of interest-for example, to enhance lineage-directed differentiation-with the use of a trap-coupled RMCE reaction. We show that, similar to ESCs, Dox-controlled expression of the cardiac transcriptional regulator Mesp1 together with Wnt inhibition enhances the generation of functional cardiomyocytes upon in vitro differentiation of such RMCE-retargeted iPSCs. This ROSA26-iPSC mouse model is therefore an excellent tool for studying both cellular reprogramming and lineage-directed differentiation factors from the same locus and will greatly facilitate the identification and ease of functional characterization of the genetic/epigenetic determinants involved in these complex processes.},
  author       = {Haenebalcke, Lieven and Goossens, Steven and Dierickx, Pieterjan and Bartunkova, Sona and D'Hont, Jinke and Haigh, Katharina and Hochepied, Tino and Wirth, Dagmar and Nagy, Andras and Haigh, Jody},
  issn         = {2211-1247},
  journal      = {CELL REPORTS},
  keyword      = {RECOMBINATION,INDUCTION,LOCUS,MODEL,TIME,MESP1},
  language     = {eng},
  number       = {2},
  pages        = {335--341},
  title        = {The ROSA26-iPSC mouse: a conditional, inducible, and exchangeable resource for studying cellular (de)differentiation},
  url          = {http://dx.doi.org/10.1016/j.celrep.2013.01.016},
  volume       = {3},
  year         = {2013},
}

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