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Diversity in bacterium-host interactions within the species Helicobacter heilmannii sensu stricto

Myrthe Joosten (UGent) , Caroline Blaecher (UGent) , Bram Flahou (UGent) , Richard Ducatelle (UGent) , Freddy Haesebrouck (UGent) and Annemieke Smet (UGent)
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Abstract
Helicobacter (H.) heilmannii sensu stricto (s.s.) is a zoonotic bacterium that naturally colonizes the stomach of dogs and cats. In humans, this microorganism has been associated with gastritis, peptic ulcer disease and mucosa associated lymphoid tissue (MALT) lymphoma. Little information is available about the pathogenesis of H. heilmannii s.s. infections in humans and it is unknown whether differences in virulence exist within this species. Therefore, a Mongolian gerbil model was used to study bacterium-host interactions of 9 H. heilmannii s.s. strains. The colonization ability of the strains, the intensity of gastritis and gene expression of various inflammatory cytokines in the stomach were determined at 9 weeks after experimental infection. The induction of an antrum-dominant chronic active gastritis with formation of lymphocytic aggregates was shown for 7 strains. High-level antral colonization was seen for 4 strains, while colonization of 4 other strains was more restricted and one strain was not detected in the stomach at 9 weeks post infection. All strains inducing a chronic active gastritis caused an up-regulation of the pro-inflammatory cytokine IL-1 beta in the antrum. A reduced antral expression of H+/K+ ATPase was seen in the stomach after infection with 3 highly colonizing strains and 2 highly colonizing strains caused an increased gastrin expression in the fundus. In none of the H. heilmannii s.s.-infected groups, IFN-gamma expression was up-regulated. This study demonstrates diversity in bacterium-host interactions within the species H. heilmannii s.s. and that the pathogenesis of gastric infections with this microorganism is not identical to that of an H. pylori infection.
Keywords
PYLORI INFECTION, MONGOLIAN GERBILS, HUMAN GASTRIC-MUCOSA, IMMUNE-RESPONSE, GENE-EXPRESSION, CELL RESPONSES, ACID-SECRETION, SPP. INFECTION, MALT LYMPHOMA, CATS

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Chicago
Joosten, Myrthe, Caroline Blaecher, Bram Flahou, Richard Ducatelle, Freddy Haesebrouck, and Annemieke Smet. 2013. “Diversity in Bacterium-host Interactions Within the Species Helicobacter Heilmannii Sensu Stricto.” Veterinary Research 44.
APA
Joosten, M., Blaecher, C., Flahou, B., Ducatelle, R., Haesebrouck, F., & Smet, A. (2013). Diversity in bacterium-host interactions within the species Helicobacter heilmannii sensu stricto. VETERINARY RESEARCH, 44.
Vancouver
1.
Joosten M, Blaecher C, Flahou B, Ducatelle R, Haesebrouck F, Smet A. Diversity in bacterium-host interactions within the species Helicobacter heilmannii sensu stricto. VETERINARY RESEARCH. 2013;44.
MLA
Joosten, Myrthe, Caroline Blaecher, Bram Flahou, et al. “Diversity in Bacterium-host Interactions Within the Species Helicobacter Heilmannii Sensu Stricto.” VETERINARY RESEARCH 44 (2013): n. pag. Print.
@article{4115235,
  abstract     = {Helicobacter (H.) heilmannii sensu stricto (s.s.) is a zoonotic bacterium that naturally colonizes the stomach of dogs and cats. In humans, this microorganism has been associated with gastritis, peptic ulcer disease and mucosa associated lymphoid tissue (MALT) lymphoma. Little information is available about the pathogenesis of H. heilmannii s.s. infections in humans and it is unknown whether differences in virulence exist within this species. Therefore, a Mongolian gerbil model was used to study bacterium-host interactions of 9 H. heilmannii s.s. strains. The colonization ability of the strains, the intensity of gastritis and gene expression of various inflammatory cytokines in the stomach were determined at 9 weeks after experimental infection. The induction of an antrum-dominant chronic active gastritis with formation of lymphocytic aggregates was shown for 7 strains. High-level antral colonization was seen for 4 strains, while colonization of 4 other strains was more restricted and one strain was not detected in the stomach at 9 weeks post infection. All strains inducing a chronic active gastritis caused an up-regulation of the pro-inflammatory cytokine IL-1 beta in the antrum. A reduced antral expression of H+/K+ ATPase was seen in the stomach after infection with 3 highly colonizing strains and 2 highly colonizing strains caused an increased gastrin expression in the fundus. In none of the H. heilmannii s.s.-infected groups, IFN-gamma expression was up-regulated. This study demonstrates diversity in bacterium-host interactions within the species H. heilmannii s.s. and that the pathogenesis of gastric infections with this microorganism is not identical to that of an H. pylori infection.},
  articleno    = {65},
  author       = {Joosten, Myrthe and Blaecher, Caroline and Flahou, Bram and Ducatelle, Richard and Haesebrouck, Freddy and Smet, Annemieke},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keywords     = {PYLORI INFECTION,MONGOLIAN GERBILS,HUMAN GASTRIC-MUCOSA,IMMUNE-RESPONSE,GENE-EXPRESSION,CELL RESPONSES,ACID-SECRETION,SPP. INFECTION,MALT LYMPHOMA,CATS},
  language     = {eng},
  pages        = {10},
  title        = {Diversity in bacterium-host interactions within the species Helicobacter heilmannii sensu stricto},
  url          = {http://dx.doi.org/10.1186/1297-9716-44-65},
  volume       = {44},
  year         = {2013},
}

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