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Visualization of the exocyst complex dynamics at the plasma membrane of Arabidopsis thaliana

(2013) MOLECULAR BIOLOGY OF THE CELL. 24(4). p.510-520
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Abstract
The exocyst complex, an effector of Rho and Rab GTPases, is believed to function as an exocytotic vesicle tether at the plasma membrane before soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex formation. Exocyst subunits localize to secretory-active regions of the plasma membrane, exemplified by the outer domain of Arabidopsis root epidermal cells. Using variable-angle epifluorescence microscopy, we visualized the dynamics of exocyst subunits at this domain. The subunits colocalized in defined foci at the plasma membrane, distinct from endocytic sites. Exocyst foci were independent of cytoskeleton, although prolonged actin disruption led to changes in exocyst localization. Exocyst foci partially overlapped with vesicles visualized by VAMP721 v-SNARE, but the majority of the foci represent sites without vesicles, as indicated by electron microscopy and drug treatments, supporting the concept of the exocyst functioning as a dynamic particle. We observed a decrease of SEC6-green fluorescent protein foci in an exo70A1 exocyst mutant. Finally, we documented decreased VAMP721 trafficking to the plasma membrane in exo70A1 and exo84b mutants. Our data support the concept that the exocyst-complex subunits dynamically dock and undock at the plasma membrane to create sites primed for vesicle tethering.
Keywords
EXOCYTOSIS, VESICLE, LIVE CELLS, CELL POLARITY, MAMMALIAN EXOCYST, PLANT TRANSFORMATION, POLARIZED SECRETION, SITES, GOLGI, ENDOCYTOSIS

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MLA
Fendrych, Matyáš et al. “Visualization of the Exocyst Complex Dynamics at the Plasma Membrane of Arabidopsis Thaliana.” MOLECULAR BIOLOGY OF THE CELL 24.4 (2013): 510–520. Print.
APA
Fendrych, M., Synek, L., Pečenková, T., Drdová, E. J., Sekereš, J., De Rycke, R., Nowack, M., et al. (2013). Visualization of the exocyst complex dynamics at the plasma membrane of Arabidopsis thaliana. MOLECULAR BIOLOGY OF THE CELL, 24(4), 510–520.
Chicago author-date
Fendrych, Matyáš, Lukáš Synek, Tamara Pečenková, Edita Janková Drdová, Juraj Sekereš, Riet De Rycke, Moritz Nowack, and Viktor Žárský. 2013. “Visualization of the Exocyst Complex Dynamics at the Plasma Membrane of Arabidopsis Thaliana.” Molecular Biology of the Cell 24 (4): 510–520.
Chicago author-date (all authors)
Fendrych, Matyáš, Lukáš Synek, Tamara Pečenková, Edita Janková Drdová, Juraj Sekereš, Riet De Rycke, Moritz Nowack, and Viktor Žárský. 2013. “Visualization of the Exocyst Complex Dynamics at the Plasma Membrane of Arabidopsis Thaliana.” Molecular Biology of the Cell 24 (4): 510–520.
Vancouver
1.
Fendrych M, Synek L, Pečenková T, Drdová EJ, Sekereš J, De Rycke R, et al. Visualization of the exocyst complex dynamics at the plasma membrane of Arabidopsis thaliana. MOLECULAR BIOLOGY OF THE CELL. 2013;24(4):510–20.
IEEE
[1]
M. Fendrych et al., “Visualization of the exocyst complex dynamics at the plasma membrane of Arabidopsis thaliana,” MOLECULAR BIOLOGY OF THE CELL, vol. 24, no. 4, pp. 510–520, 2013.
@article{4110391,
  abstract     = {The exocyst complex, an effector of Rho and Rab GTPases, is believed to function as an exocytotic vesicle tether at the plasma membrane before soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex formation. Exocyst subunits localize to secretory-active regions of the plasma membrane, exemplified by the outer domain of Arabidopsis root epidermal cells. Using variable-angle epifluorescence microscopy, we visualized the dynamics of exocyst subunits at this domain. The subunits colocalized in defined foci at the plasma membrane, distinct from endocytic sites. Exocyst foci were independent of cytoskeleton, although prolonged actin disruption led to changes in exocyst localization. Exocyst foci partially overlapped with vesicles visualized by VAMP721 v-SNARE, but the majority of the foci represent sites without vesicles, as indicated by electron microscopy and drug treatments, supporting the concept of the exocyst functioning as a dynamic particle. We observed a decrease of SEC6-green fluorescent protein foci in an exo70A1 exocyst mutant. Finally, we documented decreased VAMP721 trafficking to the plasma membrane in exo70A1 and exo84b mutants. Our data support the concept that the exocyst-complex subunits dynamically dock and undock at the plasma membrane to create sites primed for vesicle tethering.},
  author       = {Fendrych, Matyáš and Synek, Lukáš and Pečenková, Tamara and Drdová, Edita Janková and Sekereš, Juraj and De Rycke, Riet and Nowack, Moritz and Žárský, Viktor},
  issn         = {1059-1524},
  journal      = {MOLECULAR BIOLOGY OF THE CELL},
  keywords     = {EXOCYTOSIS,VESICLE,LIVE CELLS,CELL POLARITY,MAMMALIAN EXOCYST,PLANT TRANSFORMATION,POLARIZED SECRETION,SITES,GOLGI,ENDOCYTOSIS},
  language     = {eng},
  number       = {4},
  pages        = {510--520},
  title        = {Visualization of the exocyst complex dynamics at the plasma membrane of Arabidopsis thaliana},
  url          = {http://dx.doi.org/10.1091/mbc.E12-06-0492},
  volume       = {24},
  year         = {2013},
}

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