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Molecular analysis of the gut contents of Harmonia axyridis (Coleoptera: Coccinellidae) as a method for detecting intra-guild predation by this species on aphidophagous predators other than coccinellids

(2013) EUROPEAN JOURNAL OF ENTOMOLOGY. 110(4). p.567-576
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Abstract
Several studies have demonstrated that the invasive ladybird Harmonia axyridis is a strong intra-guild predator of native species of ladybird. Laboratory studies have shown that H. axyridis can be an intra-guild predator of aphid predators other than coccinellids, including the hoverfly Episyrphus balteatus and lacewing Chrysoperla carnea. However, little is known about the effect of intra-guild predation (IGP) by H. axyridis on hoverfly and lacewing populations in the field. In the present study molecular analyses were used to detect the DNA of E. balteatus and C. carnea in the gut contents of H. axyridis. Primers for the syrphid and chrysopid prey were designed and feeding experiments performed to determine how long prey DNA remains detectable in the guts of this ladybird. DNA detection was influenced by the life stage of the predator and species of prey. Meal size did not affect detection time, except when fourth instar individuals of H. axyridis were fed 10 eggs or one second instar of C. carnea. Predator weight, sex and morpho-type (melanic/non-melanic) did not influence DNA detection. The half-life of the time for which the DNA of the prey remained detectable was calculated for each predator-prey combination, and ranged from 8.9 to 52.4 h. This method can be used to study the ecological importance of IGP by H. axyridis on aphidophagous predators other than coccinellids in the field.
Keywords
PCR, DETECTABILITY, PARASITOIDS, OVIPOSITION PREFERENCE, APHID PREDATORS, BIOLOGICAL-CONTROL, INVERTEBRATE PREDATORS, GENERALIST PREDATORS, COLORADO POTATO BEETLE, PREY DNA, gut-content analysis, Episyrphus balteatus, intraguild predation, Syrphidae, Chrysoperla carnea, Diptera, Chrysopidae, Harmonia axyridis, Neuroptera, Coccinellidae, Coleoptera

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Citation

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Chicago
Ingels, Brecht, Alexandre Aebi, Louis Hautier, Thomas Van Leeuwen, and Patrick De Clercq. 2013. “Molecular Analysis of the Gut Contents of Harmonia Axyridis (Coleoptera: Coccinellidae) as a Method for Detecting Intra-guild Predation by This Species on Aphidophagous Predators Other Than Coccinellids.” European Journal of Entomology 110 (4): 567–576.
APA
Ingels, B., Aebi, A., Hautier, L., Van Leeuwen, T., & De Clercq, P. (2013). Molecular analysis of the gut contents of Harmonia axyridis (Coleoptera: Coccinellidae) as a method for detecting intra-guild predation by this species on aphidophagous predators other than coccinellids. EUROPEAN JOURNAL OF ENTOMOLOGY, 110(4), 567–576.
Vancouver
1.
Ingels B, Aebi A, Hautier L, Van Leeuwen T, De Clercq P. Molecular analysis of the gut contents of Harmonia axyridis (Coleoptera: Coccinellidae) as a method for detecting intra-guild predation by this species on aphidophagous predators other than coccinellids. EUROPEAN JOURNAL OF ENTOMOLOGY. 2013;110(4):567–76.
MLA
Ingels, Brecht, Alexandre Aebi, Louis Hautier, et al. “Molecular Analysis of the Gut Contents of Harmonia Axyridis (Coleoptera: Coccinellidae) as a Method for Detecting Intra-guild Predation by This Species on Aphidophagous Predators Other Than Coccinellids.” EUROPEAN JOURNAL OF ENTOMOLOGY 110.4 (2013): 567–576. Print.
@article{4108078,
  abstract     = {Several studies have demonstrated that the invasive ladybird Harmonia axyridis is a strong intra-guild predator of native species of ladybird. Laboratory studies have shown that H. axyridis can be an intra-guild predator of aphid predators other than coccinellids, including the hoverfly Episyrphus balteatus and lacewing Chrysoperla carnea. However, little is known about the effect of intra-guild predation (IGP) by H. axyridis on hoverfly and lacewing populations in the field. In the present study molecular analyses were used to detect the DNA of E. balteatus and C. carnea in the gut contents of H. axyridis. Primers for the syrphid and chrysopid prey were designed and feeding experiments performed to determine how long prey DNA remains detectable in the guts of this ladybird. DNA detection was influenced by the life stage of the predator and species of prey. Meal size did not affect detection time, except when fourth instar individuals of H. axyridis were fed 10 eggs or one second instar of C. carnea. Predator weight, sex and morpho-type (melanic/non-melanic) did not influence DNA detection. The half-life of the time for which the DNA of the prey remained detectable was calculated for each predator-prey combination, and ranged from 8.9 to 52.4 h. This method can be used to study the ecological importance of IGP by H. axyridis on aphidophagous predators other than coccinellids in the field.},
  author       = {Ingels, Brecht and Aebi, Alexandre and Hautier, Louis and Van Leeuwen, Thomas and De Clercq, Patrick},
  issn         = {1210-5759},
  journal      = {EUROPEAN JOURNAL OF ENTOMOLOGY},
  language     = {eng},
  number       = {4},
  pages        = {567--576},
  title        = {Molecular analysis of the gut contents of Harmonia axyridis (Coleoptera: Coccinellidae) as a method for detecting intra-guild predation by this species on aphidophagous predators other than coccinellids},
  url          = {http://www.eje.cz/pdfs/110/4/567},
  volume       = {110},
  year         = {2013},
}

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