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Helicobacter baculiformis sp nov., isolated from feline stomach mucosa

Margo Baele UGent, Annemie Decostere UGent, Peter Vandamme UGent, Kathleen Van den Bulck, I Gruntar, J Mehle, J Mast, Richard Ducatelle UGent and Freddy Haesebrouck UGent (2008) INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 58(2). p.357-364
abstract
A Gram-negative, microaerophilic slender rod, measuring approximately 10 mu m long and approximately 1 mu m wide, isolated from the gastric mucosa of a cat and designated strain M50(T), was subjected to a polyphasic taxonomic study. Despite its apparent lack of helical coils, the organism showed a corkscrew-like motion by means of multiple sheathed flagella located at both ends of the cell and by a periplasmic fibril coiled around the body. Strain M50T grew preferably on biphasic culture plates or on very moist agar. Coccoid forms predominatecl in cultures older than 4 days as well as in growth obtained on dry agar plates. The strain grew at 37 degrees C, but not at 25 or 42 degrees C and exhibited urease, oxidase and catalase activities. On the basis of 16S rRNA gene sequence analysis, the novel isolate was identified as a member of the genus Helicobacter and showed about 98 to 99% sequence similarity to Helicobacter felis, Helicobacter bizzozeronii, Helicobacter salomonis, Helicobacter cynogastricus and 'Candidatus Helicobacter heilmannii', five highly related species previously detected in the feline or canine gastric mucosa. Protein profiling of strain M50(T) using SDS-PAGE revealed a pattern different from those of other Helicobacter species of mammalian gastric origin. Additionally, the urease and HSP60 gene sequences of strain M50(T) were different from those of H. felis, H. bizzozeronii, H. salomonis, H. cynogastricus and 'Ca. H. heilmannii'. It is thus proposed that strain M50(T) (=LMG T 23839 =CCUG 53816(T)) represents a novel species within this genus, for which the name Helicobacter baculiformis sp. nov. is proposed.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GASTRIC-MUCOSA, GENUS HELICOBACTER, RIBOSOMAL-RNA, CANINE, BIZZOZERONII, IDENTIFICATION, HEILMANNII, BACTERIUM, STRAINS, 16S
journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Int. J. Syst. Evol. Microbiol.
volume
58
issue
2
pages
357 - 364
Web of Science type
Article
Web of Science id
000253563800008
JCR category
MICROBIOLOGY
JCR impact factor
1.463 (2008)
JCR rank
68/91 (2008)
JCR quartile
3 (2008)
ISSN
1466-5026
DOI
10.1099/ijs.0.65152-0
language
English
UGent publication?
yes
classification
A1
id
410410
handle
http://hdl.handle.net/1854/LU-410410
date created
2008-05-16 16:45:00
date last changed
2016-12-19 15:42:23
@article{410410,
  abstract     = {A Gram-negative, microaerophilic slender rod, measuring approximately 10 mu m long and approximately 1 mu m wide, isolated from the gastric mucosa of a cat and designated strain M50(T), was subjected to a polyphasic taxonomic study. Despite its apparent lack of helical coils, the organism showed a corkscrew-like motion by means of multiple sheathed flagella located at both ends of the cell and by a periplasmic fibril coiled around the body. Strain M50T grew preferably on biphasic culture plates or on very moist agar. Coccoid forms predominatecl in cultures older than 4 days as well as in growth obtained on dry agar plates. The strain grew at 37 degrees C, but not at 25 or 42 degrees C and exhibited urease, oxidase and catalase activities. On the basis of 16S rRNA gene sequence analysis, the novel isolate was identified as a member of the genus Helicobacter and showed about 98 to 99\% sequence similarity to Helicobacter felis, Helicobacter bizzozeronii, Helicobacter salomonis, Helicobacter cynogastricus and 'Candidatus Helicobacter heilmannii', five highly related species previously detected in the feline or canine gastric mucosa. Protein profiling of strain M50(T) using SDS-PAGE revealed a pattern different from those of other Helicobacter species of mammalian gastric origin. Additionally, the urease and HSP60 gene sequences of strain M50(T) were different from those of H. felis, H. bizzozeronii, H. salomonis, H. cynogastricus and 'Ca. H. heilmannii'. It is thus proposed that strain M50(T) (=LMG T 23839 =CCUG 53816(T)) represents a novel species within this genus, for which the name Helicobacter baculiformis sp. nov. is proposed.},
  author       = {Baele, Margo and Decostere, Annemie and Vandamme, Peter and Van den Bulck, Kathleen and Gruntar, I and Mehle, J and Mast, J and Ducatelle, Richard and Haesebrouck, Freddy},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keyword      = {GASTRIC-MUCOSA,GENUS HELICOBACTER,RIBOSOMAL-RNA,CANINE,BIZZOZERONII,IDENTIFICATION,HEILMANNII,BACTERIUM,STRAINS,16S},
  language     = {eng},
  number       = {2},
  pages        = {357--364},
  title        = {Helicobacter baculiformis sp nov., isolated from feline stomach mucosa},
  url          = {http://dx.doi.org/10.1099/ijs.0.65152-0},
  volume       = {58},
  year         = {2008},
}

Chicago
Baele, Margo, Annemie Decostere, Peter Vandamme, Kathleen Van den Bulck, I Gruntar, J Mehle, J Mast, Richard Ducatelle, and Freddy Haesebrouck. 2008. “Helicobacter Baculiformis Sp Nov., Isolated from Feline Stomach Mucosa.” International Journal of Systematic and Evolutionary Microbiology 58 (2): 357–364.
APA
Baele, M., Decostere, A., Vandamme, P., Van den Bulck, K., Gruntar, I., Mehle, J., Mast, J., et al. (2008). Helicobacter baculiformis sp nov., isolated from feline stomach mucosa. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 58(2), 357–364.
Vancouver
1.
Baele M, Decostere A, Vandamme P, Van den Bulck K, Gruntar I, Mehle J, et al. Helicobacter baculiformis sp nov., isolated from feline stomach mucosa. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2008;58(2):357–64.
MLA
Baele, Margo, Annemie Decostere, Peter Vandamme, et al. “Helicobacter Baculiformis Sp Nov., Isolated from Feline Stomach Mucosa.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 58.2 (2008): 357–364. Print.