Advanced search
1 file | 1.50 MB Add to list

Salicylic acid interferes with clathrin-mediated endocytic protein trafficking

Author
Organization
Abstract
Removal of cargos from the cell surface via endocytosis is an efficient mechanism to regulate activities of plasma membrane (PM)-resident proteins, such as receptors or transporters. Salicylic acid (SA) is an important plant hormone that is traditionally associated with pathogen defense. Here, we describe an unanticipated effect of SA on subcellular endocytic cycling of proteins. Both exogenous treatments and endogenously enhanced SA levels repressed endocytosis of different PM proteins. The SA effect on endocytosis did not involve transcription or known components of the SA signaling pathway for transcriptional regulation. SA likely targets an endocytic mechanism that involves the coat protein clathrin, because SA interfered with the clathrin incidence at the PM and clathrin-deficient mutants were less sensitive to the impact of SA on the auxin distribution and root bending during the gravitropic response. By contrast, SA did not affect the ligand-induced endocytosis of the FLAGELLIN SENSING2 (FLS2) receptor during pathogen responses. Our data suggest that the established SA impact on transcription in plant immunity and the nontranscriptional effect of SA on clathrin-mediated endocytosis are independent mechanisms by which SA regulates distinct aspects of plant physiology.
Keywords
ARABIDOPSIS ROOTS, RECEPTOR FLS2, DISEASE RESISTANCE, DEPENDENT ENDOCYTOSIS, PLANT DEFENSE, AUXIN SIGNALING PATHWAY, SYSTEMIC ACQUIRED-RESISTANCE, GROWTH, EFFLUX, CELLS

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.50 MB

Citation

Please use this url to cite or link to this publication:

MLA
Du, Yunlong, Ricardo Tejos Ulloa, Martina Beck, et al. “Salicylic Acid Interferes with Clathrin-mediated Endocytic Protein Trafficking.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 110.19 (2013): 7946–7951. Print.
APA
Du, Y., Tejos Ulloa, R., Beck, M., Himschoot, E., Li, H., Robatzek, S., Vanneste, S., et al. (2013). Salicylic acid interferes with clathrin-mediated endocytic protein trafficking. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 110(19), 7946–7951.
Chicago author-date
Du, Yunlong, Ricardo Tejos Ulloa, Martina Beck, Ellie Himschoot, Hongjiang Li, Silke Robatzek, Steffen Vanneste, and Jiri Friml. 2013. “Salicylic Acid Interferes with Clathrin-mediated Endocytic Protein Trafficking.” Proceedings of the National Academy of Sciences of the United States of America 110 (19): 7946–7951.
Chicago author-date (all authors)
Du, Yunlong, Ricardo Tejos Ulloa, Martina Beck, Ellie Himschoot, Hongjiang Li, Silke Robatzek, Steffen Vanneste, and Jiri Friml. 2013. “Salicylic Acid Interferes with Clathrin-mediated Endocytic Protein Trafficking.” Proceedings of the National Academy of Sciences of the United States of America 110 (19): 7946–7951.
Vancouver
1.
Du Y, Tejos Ulloa R, Beck M, Himschoot E, Li H, Robatzek S, et al. Salicylic acid interferes with clathrin-mediated endocytic protein trafficking. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2013;110(19):7946–51.
IEEE
[1]
Y. Du et al., “Salicylic acid interferes with clathrin-mediated endocytic protein trafficking,” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 110, no. 19, pp. 7946–7951, 2013.
@article{4098710,
  abstract     = {{Removal of cargos from the cell surface via endocytosis is an efficient mechanism to regulate activities of plasma membrane (PM)-resident proteins, such as receptors or transporters. Salicylic acid (SA) is an important plant hormone that is traditionally associated with pathogen defense. Here, we describe an unanticipated effect of SA on subcellular endocytic cycling of proteins. Both exogenous treatments and endogenously enhanced SA levels repressed endocytosis of different PM proteins. The SA effect on endocytosis did not involve transcription or known components of the SA signaling pathway for transcriptional regulation. SA likely targets an endocytic mechanism that involves the coat protein clathrin, because SA interfered with the clathrin incidence at the PM and clathrin-deficient mutants were less sensitive to the impact of SA on the auxin distribution and root bending during the gravitropic response. By contrast, SA did not affect the ligand-induced endocytosis of the FLAGELLIN SENSING2 (FLS2) receptor during pathogen responses. Our data suggest that the established SA impact on transcription in plant immunity and the nontranscriptional effect of SA on clathrin-mediated endocytosis are independent mechanisms by which SA regulates distinct aspects of plant physiology.}},
  author       = {{Du, Yunlong and Tejos Ulloa, Ricardo and Beck, Martina and Himschoot, Ellie and Li, Hongjiang and Robatzek, Silke and Vanneste, Steffen and Friml, Jiri}},
  issn         = {{0027-8424}},
  journal      = {{PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}},
  keywords     = {{ARABIDOPSIS ROOTS,RECEPTOR FLS2,DISEASE RESISTANCE,DEPENDENT ENDOCYTOSIS,PLANT DEFENSE,AUXIN SIGNALING PATHWAY,SYSTEMIC ACQUIRED-RESISTANCE,GROWTH,EFFLUX,CELLS}},
  language     = {{eng}},
  number       = {{19}},
  pages        = {{7946--7951}},
  title        = {{Salicylic acid interferes with clathrin-mediated endocytic protein trafficking}},
  url          = {{http://dx.doi.org/10.1073/pnas.1220205110}},
  volume       = {{110}},
  year         = {{2013}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: