Advanced search
Add to list

Inheritance of foreign genes in transgenic azalea plants generated by Agrobacterium-mediated transformation

(2000) Acta Horticulturae. 521. p.127-132
Author
Organization
Abstract
The evergreen azalea (Rhododendron simsii Planch.) is a very important ornamental crop in Belgium and Western Europe. A lot of the possibilities of conventional breeding have been explored in the last decades. The different Rhododendron species show a high degree of phenotypic variation. But the combination of certain traits from these plants in a new azalea cultivar is not possible, because of crossing barriers. Therefore the introduction of specific new traits in the evergreen azalea via classical breeding is difficult. An alternative strategy to broaden the genetic variability is to make use of genetic engineering. Transformation of evergreen azalea was carried out using Agrobacterium tumefaciens AGLOpMOG410, containing the beta-glucuronidase (gus) - intron reporter gene and the neomycin phosphotransferase II (nptII) gene. Fertile transgenic azalea plants were obtained. Transformants were morphologically identical to untransformed plants. The expression of the gus gene in the primary transgenic plants was demonstrated by Northern blot analysis and histochemical GUS assays. Besides genetic transformation of the plants was confirmed by their ability to produce callus and to regenerate on Anderson medium containing kanamycin. The inheritance of the foreign marker genes in sexually derived progeny was tested. Segregation of the gus gene occurred in a Mendelian way.
Keywords
Rhododendron simsii, Agrobacterium tumefaciens, azalea, genetic transformation, transgene inheritance, RHODODENDRON, DNA

Citation

Please use this url to cite or link to this publication:

MLA
Mertens, M, J Heursel, Erik Van Bockstaele, et al. “Inheritance of Foreign Genes in Transgenic Azalea Plants Generated by Agrobacterium-mediated Transformation.” Acta Horticulturae. Ed. LHW van der Plas et al. Vol. 521. Leuven, Belgium: International Society for Horticultural Science (ISHS), 2000. 127–132. Print.
APA
Mertens, M, Heursel, J., Van Bockstaele, E., & De Loose, M. (2000). Inheritance of foreign genes in transgenic azalea plants generated by Agrobacterium-mediated transformation. In L. van der Plas, J. Dons, J. Vanderleyden, & M. De Loose (Eds.), Acta Horticulturae (Vol. 521, pp. 127–132). Presented at the 25th International Horticultural Congress, Leuven, Belgium: International Society for Horticultural Science (ISHS).
Chicago author-date
Mertens, M, J Heursel, Erik Van Bockstaele, and Marc De Loose. 2000. “Inheritance of Foreign Genes in Transgenic Azalea Plants Generated by Agrobacterium-mediated Transformation.” In Acta Horticulturae, ed. LHW van der Plas, JJM Dons, J Vanderleyden, and Marc De Loose, 521:127–132. Leuven, Belgium: International Society for Horticultural Science (ISHS).
Chicago author-date (all authors)
Mertens, M, J Heursel, Erik Van Bockstaele, and Marc De Loose. 2000. “Inheritance of Foreign Genes in Transgenic Azalea Plants Generated by Agrobacterium-mediated Transformation.” In Acta Horticulturae, ed. LHW van der Plas, JJM Dons, J Vanderleyden, and Marc De Loose, 521:127–132. Leuven, Belgium: International Society for Horticultural Science (ISHS).
Vancouver
1.
Mertens M, Heursel J, Van Bockstaele E, De Loose M. Inheritance of foreign genes in transgenic azalea plants generated by Agrobacterium-mediated transformation. In: van der Plas L, Dons J, Vanderleyden J, De Loose M, editors. Acta Horticulturae. Leuven, Belgium: International Society for Horticultural Science (ISHS); 2000. p. 127–32.
IEEE
[1]
M. Mertens, J. Heursel, E. Van Bockstaele, and M. De Loose, “Inheritance of foreign genes in transgenic azalea plants generated by Agrobacterium-mediated transformation,” in Acta Horticulturae, Brussels, Belgium, 2000, vol. 521, pp. 127–132.
@inproceedings{409860,
  abstract     = {The evergreen azalea (Rhododendron simsii Planch.) is a very important ornamental crop in Belgium and Western Europe. A lot of the possibilities of conventional breeding have been explored in the last decades. The different Rhododendron species show a high degree of phenotypic variation. But the combination of certain traits from these plants in a new azalea cultivar is not possible, because of crossing barriers. Therefore the introduction of specific new traits in the evergreen azalea via classical breeding is difficult. An alternative strategy to broaden the genetic variability is to make use of genetic engineering. 
Transformation of evergreen azalea was carried out using Agrobacterium tumefaciens AGLOpMOG410, containing the beta-glucuronidase (gus) - intron reporter gene and the neomycin phosphotransferase II (nptII) gene. 
Fertile transgenic azalea plants were obtained. Transformants were morphologically identical to untransformed plants. The expression of the gus gene in the primary transgenic plants was demonstrated by Northern blot analysis and histochemical GUS assays. Besides genetic transformation of the plants was confirmed by their ability to produce callus and to regenerate on Anderson medium containing kanamycin. The inheritance of the foreign marker genes in sexually derived progeny was tested. Segregation of the gus gene occurred in a Mendelian way.},
  author       = {Mertens, M and Heursel, J and Van Bockstaele, Erik and De Loose, Marc},
  booktitle    = {Acta Horticulturae},
  editor       = {van der Plas, LHW and Dons, JJM and Vanderleyden, J and De Loose, Marc},
  isbn         = {9789066058538},
  issn         = {0567-7572},
  keywords     = {Rhododendron simsii,Agrobacterium tumefaciens,azalea,genetic transformation,transgene inheritance,RHODODENDRON,DNA},
  language     = {eng},
  location     = {Brussels, Belgium},
  pages        = {127--132},
  publisher    = {International Society for Horticultural Science (ISHS)},
  title        = {Inheritance of foreign genes in transgenic azalea plants generated by Agrobacterium-mediated transformation},
  url          = {http://www.actahort.org/books/521/521_13.htm},
  volume       = {521},
  year         = {2000},
}

Web of Science
Times cited: