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Cytokinins act directly on lateral root founder cells to inhibit root initiation

Laurent Laplaze, Eva Benkova UGent, Ilda Casimiro, Lies Maes UGent, Steffen Vanneste UGent, Ranjan Swarup, Dolf Weijers, Vanessa Calvo, Boris Parizot UGent and Maria Begoña Herrera-Rodriguez, et al. (2007) PLANT CELL. 19(12). p.3889-3900
abstract
In Arabidopsis thaliana, lateral roots are formed from root pericycle cells adjacent to the xylem poles. Lateral root development is regulated antagonistically by the plant hormones auxin and cytokinin. While a great deal is known about how auxin promotes lateral root development, the mechanism of cytokinin repression is still unclear. Elevating cytokinin levels was observed to disrupt lateral root initiation and the regular pattern of divisions that characterizes lateral root development in Arabidopsis. To identify the stage of lateral root development that is sensitive to cytokinins, we targeted the expression of the Agrobacterium tumefaciens cytokinin biosynthesis enzyme isopentenyltransferase to either xylem-pole pericycle cells or young lateral root primordia using GAL4-GFP enhancer trap lines. Transactivation experiments revealed that xylem-pole pericycle cells are sensitive to cytokinins, whereas young lateral root primordia are not. This effect is physiologically significant because transactivation of the Arabidopsis cytokinin degrading enzyme cytokinin oxidase 1 in lateral root founder cells results in increased lateral root formation. We observed that cytokinins perturb the expression of PIN genes in lateral root founder cells and prevent the formation of an auxin gradient that is required to pattern lateral root primordia.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SHOOT, GENE, AUXIN, PEA ROOTS, TOBACCO PLANTS, ARABIDOPSIS-THALIANA, RESPONSE REGULATORS, EXPRESSION, PERICYCLE, MERISTEM
journal title
PLANT CELL
Plant Cell
volume
19
issue
12
pages
3889 - 3900
Web of Science type
Article
Web of Science id
000252699300007
JCR category
PLANT SCIENCES
JCR impact factor
9.653 (2007)
JCR rank
3/149 (2007)
JCR quartile
1 (2007)
ISSN
1040-4651
DOI
10.1105/tpc.107.055863
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
409497
handle
http://hdl.handle.net/1854/LU-409497
date created
2008-05-15 18:56:00
date last changed
2012-12-18 13:49:21
@article{409497,
  abstract     = {In Arabidopsis thaliana, lateral roots are formed from root pericycle cells adjacent to the xylem poles. Lateral root development is regulated antagonistically by the plant hormones auxin and cytokinin. While a great deal is known about how auxin promotes lateral root development, the mechanism of cytokinin repression is still unclear. Elevating cytokinin levels was observed to disrupt lateral root initiation and the regular pattern of divisions that characterizes lateral root development in Arabidopsis. To identify the stage of lateral root development that is sensitive to cytokinins, we targeted the expression of the Agrobacterium tumefaciens cytokinin biosynthesis enzyme isopentenyltransferase to either xylem-pole pericycle cells or young lateral root primordia using GAL4-GFP enhancer trap lines. Transactivation experiments revealed that xylem-pole pericycle cells are sensitive to cytokinins, whereas young lateral root primordia are not. This effect is physiologically significant because transactivation of the Arabidopsis cytokinin degrading enzyme cytokinin oxidase 1 in lateral root founder cells results in increased lateral root formation. We observed that cytokinins perturb the expression of PIN genes in lateral root founder cells and prevent the formation of an auxin gradient that is required to pattern lateral root primordia.},
  author       = {Laplaze, Laurent and Benkova, Eva and Casimiro, Ilda and Maes, Lies and Vanneste, Steffen and Swarup, Ranjan and Weijers, Dolf and Calvo, Vanessa and Parizot, Boris and Herrera-Rodriguez, Maria Bego{\~n}a and Offringa, Remco and Graham, Neil and Doumas, Patrick and Friml, Jiri and Bogusz, Didier and Beeckman, Tom and Bennett, Malcolm},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {SHOOT,GENE,AUXIN,PEA ROOTS,TOBACCO PLANTS,ARABIDOPSIS-THALIANA,RESPONSE REGULATORS,EXPRESSION,PERICYCLE,MERISTEM},
  language     = {eng},
  number       = {12},
  pages        = {3889--3900},
  title        = {Cytokinins act directly on lateral root founder cells to inhibit root initiation},
  url          = {http://dx.doi.org/10.1105/tpc.107.055863},
  volume       = {19},
  year         = {2007},
}

Chicago
Laplaze, Laurent, Eva Benkova, Ilda Casimiro, Lies Maes, Steffen Vanneste, Ranjan Swarup, Dolf Weijers, et al. 2007. “Cytokinins Act Directly on Lateral Root Founder Cells to Inhibit Root Initiation.” Plant Cell 19 (12): 3889–3900.
APA
Laplaze, L., Benkova, E., Casimiro, I., Maes, L., Vanneste, S., Swarup, R., Weijers, D., et al. (2007). Cytokinins act directly on lateral root founder cells to inhibit root initiation. PLANT CELL, 19(12), 3889–3900.
Vancouver
1.
Laplaze L, Benkova E, Casimiro I, Maes L, Vanneste S, Swarup R, et al. Cytokinins act directly on lateral root founder cells to inhibit root initiation. PLANT CELL. 2007;19(12):3889–900.
MLA
Laplaze, Laurent, Eva Benkova, Ilda Casimiro, et al. “Cytokinins Act Directly on Lateral Root Founder Cells to Inhibit Root Initiation.” PLANT CELL 19.12 (2007): 3889–3900. Print.