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Cytokinins act directly on lateral root founder cells to inhibit root initiation

(2007) PLANT CELL. 19(12). p.3889-3900
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Abstract
In Arabidopsis thaliana, lateral roots are formed from root pericycle cells adjacent to the xylem poles. Lateral root development is regulated antagonistically by the plant hormones auxin and cytokinin. While a great deal is known about how auxin promotes lateral root development, the mechanism of cytokinin repression is still unclear. Elevating cytokinin levels was observed to disrupt lateral root initiation and the regular pattern of divisions that characterizes lateral root development in Arabidopsis. To identify the stage of lateral root development that is sensitive to cytokinins, we targeted the expression of the Agrobacterium tumefaciens cytokinin biosynthesis enzyme isopentenyltransferase to either xylem-pole pericycle cells or young lateral root primordia using GAL4-GFP enhancer trap lines. Transactivation experiments revealed that xylem-pole pericycle cells are sensitive to cytokinins, whereas young lateral root primordia are not. This effect is physiologically significant because transactivation of the Arabidopsis cytokinin degrading enzyme cytokinin oxidase 1 in lateral root founder cells results in increased lateral root formation. We observed that cytokinins perturb the expression of PIN genes in lateral root founder cells and prevent the formation of an auxin gradient that is required to pattern lateral root primordia.
Keywords
SHOOT, GENE, AUXIN, PEA ROOTS, TOBACCO PLANTS, ARABIDOPSIS-THALIANA, RESPONSE REGULATORS, EXPRESSION, PERICYCLE, MERISTEM

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MLA
Laplaze, Laurent, Eva Benkova, Ilda Casimiro, et al. “Cytokinins Act Directly on Lateral Root Founder Cells to Inhibit Root Initiation.” PLANT CELL 19.12 (2007): 3889–3900. Print.
APA
Laplaze, L., Benkova, E., Casimiro, I., Maes, L., Vanneste, S., Swarup, R., Weijers, D., et al. (2007). Cytokinins act directly on lateral root founder cells to inhibit root initiation. PLANT CELL, 19(12), 3889–3900.
Chicago author-date
Laplaze, Laurent, Eva Benkova, Ilda Casimiro, Lies Maes, Steffen Vanneste, Ranjan Swarup, Dolf Weijers, et al. 2007. “Cytokinins Act Directly on Lateral Root Founder Cells to Inhibit Root Initiation.” Plant Cell 19 (12): 3889–3900.
Chicago author-date (all authors)
Laplaze, Laurent, Eva Benkova, Ilda Casimiro, Lies Maes, Steffen Vanneste, Ranjan Swarup, Dolf Weijers, Vanessa Calvo, Boris Parizot, Maria Begoña Herrera-Rodriguez, Remco Offringa, Neil Graham, Patrick Doumas, Jiri Friml, Didier Bogusz, Tom Beeckman, and Malcolm Bennett. 2007. “Cytokinins Act Directly on Lateral Root Founder Cells to Inhibit Root Initiation.” Plant Cell 19 (12): 3889–3900.
Vancouver
1.
Laplaze L, Benkova E, Casimiro I, Maes L, Vanneste S, Swarup R, et al. Cytokinins act directly on lateral root founder cells to inhibit root initiation. PLANT CELL. 2007;19(12):3889–900.
IEEE
[1]
L. Laplaze et al., “Cytokinins act directly on lateral root founder cells to inhibit root initiation,” PLANT CELL, vol. 19, no. 12, pp. 3889–3900, 2007.
@article{409497,
  abstract     = {In Arabidopsis thaliana, lateral roots are formed from root pericycle cells adjacent to the xylem poles. Lateral root development is regulated antagonistically by the plant hormones auxin and cytokinin. While a great deal is known about how auxin promotes lateral root development, the mechanism of cytokinin repression is still unclear. Elevating cytokinin levels was observed to disrupt lateral root initiation and the regular pattern of divisions that characterizes lateral root development in Arabidopsis. To identify the stage of lateral root development that is sensitive to cytokinins, we targeted the expression of the Agrobacterium tumefaciens cytokinin biosynthesis enzyme isopentenyltransferase to either xylem-pole pericycle cells or young lateral root primordia using GAL4-GFP enhancer trap lines. Transactivation experiments revealed that xylem-pole pericycle cells are sensitive to cytokinins, whereas young lateral root primordia are not. This effect is physiologically significant because transactivation of the Arabidopsis cytokinin degrading enzyme cytokinin oxidase 1 in lateral root founder cells results in increased lateral root formation. We observed that cytokinins perturb the expression of PIN genes in lateral root founder cells and prevent the formation of an auxin gradient that is required to pattern lateral root primordia.},
  author       = {Laplaze, Laurent and Benkova, Eva and Casimiro, Ilda and Maes, Lies and Vanneste, Steffen and Swarup, Ranjan and Weijers, Dolf and Calvo, Vanessa and Parizot, Boris and Herrera-Rodriguez, Maria Begoña and Offringa, Remco and Graham, Neil and Doumas, Patrick and Friml, Jiri and Bogusz, Didier and Beeckman, Tom and Bennett, Malcolm},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keywords     = {SHOOT,GENE,AUXIN,PEA ROOTS,TOBACCO PLANTS,ARABIDOPSIS-THALIANA,RESPONSE REGULATORS,EXPRESSION,PERICYCLE,MERISTEM},
  language     = {eng},
  number       = {12},
  pages        = {3889--3900},
  title        = {Cytokinins act directly on lateral root founder cells to inhibit root initiation},
  url          = {http://dx.doi.org/10.1105/tpc.107.055863},
  volume       = {19},
  year         = {2007},
}

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