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Stability of the T-DNA flanking regions in transgenic Arabidopsis thaliana plants under influence of abiotic stress and cultivation practices

(2008) PLANT CELL REPORTS. 27(4). p.749-757
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Abstract
Genetic transformation is often associated with different rearrangements of the plant genome at the site of insertion. Therefore the question remains weather these T-DNA insertion sites are more prone to genotoxic stresses. Here, we studied the impact of propagation through generations, the influence of gene stacking and of photo oxidative stress caused by high light intensity on the stability of the transgene flanking regions in the model plant Arabidopsis thaliana. Conformational Sensitive Capillary Electrophoresis (CSCE), RFLP and sequencing were deployed in this analysis in order to study the proximal 100 bp and the long-range T-DNA flanking sequences. By screening seven transgenic lines no evidence for occurrence of mutation events were found, implying that the nucleotide sequence of the T-DNA flanking regions of the studied events is unlikely to be unstable.
Keywords
single nucleotide polymorphisms (SNPs), T-DNA flanking regions, T-DNA insertion, GMO, Arabidopsis thaliana, GENOME, INTEGRATION, MUTATIONS, IDENTIFICATION, REARRANGEMENTS, TRANSFORMATION, INSERTIONS, FREQUENCY, RICE

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Citation

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Chicago
Papazova, Nina, Rim Ghedira, Sabine Van Glabeke, Aghleb Bartegi, Pieter Windels, Isabel Taverniers, Isabel Roldàn-Ruiz, et al. 2008. “Stability of the T-DNA Flanking Regions in Transgenic Arabidopsis Thaliana Plants Under Influence of Abiotic Stress and Cultivation Practices.” Plant Cell Reports 27 (4): 749–757.
APA
Papazova, N., Ghedira, R., Van Glabeke, S., Bartegi, A., Windels, P., Taverniers, I., Roldàn-Ruiz, I., et al. (2008). Stability of the T-DNA flanking regions in transgenic Arabidopsis thaliana plants under influence of abiotic stress and cultivation practices. PLANT CELL REPORTS, 27(4), 749–757.
Vancouver
1.
Papazova N, Ghedira R, Van Glabeke S, Bartegi A, Windels P, Taverniers I, et al. Stability of the T-DNA flanking regions in transgenic Arabidopsis thaliana plants under influence of abiotic stress and cultivation practices. PLANT CELL REPORTS. 2008;27(4):749–57.
MLA
Papazova, Nina, Rim Ghedira, Sabine Van Glabeke, et al. “Stability of the T-DNA Flanking Regions in Transgenic Arabidopsis Thaliana Plants Under Influence of Abiotic Stress and Cultivation Practices.” PLANT CELL REPORTS 27.4 (2008): 749–757. Print.
@article{409460,
  abstract     = {Genetic transformation is often associated with different rearrangements of the plant genome at the site of insertion. Therefore the question remains weather these T-DNA insertion sites are more prone to genotoxic stresses. Here, we studied the impact of propagation through generations, the influence of gene stacking and of photo oxidative stress caused by high light intensity on the stability of the transgene flanking regions in the model plant Arabidopsis thaliana. Conformational Sensitive Capillary Electrophoresis (CSCE), RFLP and sequencing were deployed in this analysis in order to study the proximal 100 bp and the long-range T-DNA flanking sequences. By screening seven transgenic lines no evidence for occurrence of mutation events were found, implying that the nucleotide sequence of the T-DNA flanking regions of the studied events is unlikely to be unstable.},
  author       = {Papazova, Nina and Ghedira, Rim and Van Glabeke, Sabine and Bartegi, Aghleb and Windels, Pieter and Taverniers, Isabel and Roldàn-Ruiz, Isabel and Van Bockstaele, Erik and Milcamps, Anne and Van den Eede, Guy and Depicker, Anna and De Loose, Marc},
  issn         = {0721-7714},
  journal      = {PLANT CELL REPORTS},
  keywords     = {single nucleotide polymorphisms (SNPs),T-DNA flanking regions,T-DNA insertion,GMO,Arabidopsis thaliana,GENOME,INTEGRATION,MUTATIONS,IDENTIFICATION,REARRANGEMENTS,TRANSFORMATION,INSERTIONS,FREQUENCY,RICE},
  language     = {eng},
  number       = {4},
  pages        = {749--757},
  title        = {Stability of the T-DNA flanking regions in transgenic Arabidopsis thaliana plants under influence of abiotic stress and cultivation practices},
  url          = {http://dx.doi.org/10.1007/s00299-007-0495-4},
  volume       = {27},
  year         = {2008},
}

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