Rhodococcus fascians infection accelerates progression of tobacco BY-2 cells into mitosis through rapid changes in plant gene expression
- Author
- Olivier Vandeputte, Danny Vereecke (UGent) , Adeline Mol, Marc Lenjou, Dirk Van Bockstaele, Mondher El Jaziri and Marie Baucher (UGent)
- Organization
- Abstract
- To characterize plant cell cycle activation following Rhodococcus fascians infection, bacterial impact on cell cycle progression of tobacco BY-2 cells was investigated. S-phase-synchronized BY-2 cells were cocultivated with R. fascians and cell cycle progression was monitored by measuring mitotic index, cell cycle gene expression and flow cytometry parameters. Cell cycle alteration was further investigated by cDNA-AFLP (amplified fragment length polymorphism). It was shown that cell cycle progression of BY-2 cells was accelerated only upon infection with bacteria whose virulence gene expression was induced by a leafy gall extract. Thirty-eight BY-2 genes showed a differential expression within 6 h post-infection. Among these, seven were previously associated with specific plant cell cycle phases (in particular S and G2/M phases). Several genes also showed a differential expression during leafy gall formation. R. fascians-infected BY-2 cells provide a simple model to identify plant genes related to leafy gall development. R. fascians can also be regarded as a useful biotic agent to alter cell cycle progression and, thereby, gain a better understanding of cell cycle regulation in plants.
- Keywords
- PROTEIN, REAL-TIME, TRANSITION, TOPOLOGY PREDICTION, ABSCISIC-ACID, S-PHASE, DIFFERENTIAL REGULATION, D-TYPE CYCLIN, LEAFY GALL FORMATION, ZEATIN TYPE CYTOKININS, Rhodococcus fascians, plant-bacteria interactions, leafy gall, hyperplasia, cell cycle, BY-2, cDNA-AFLP (amplified fragment length polymorphism)
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-409134
- MLA
- Vandeputte, Olivier, et al. “Rhodococcus Fascians Infection Accelerates Progression of Tobacco BY-2 Cells into Mitosis through Rapid Changes in Plant Gene Expression.” NEW PHYTOLOGIST, vol. 175, no. 1, 2007, pp. 140–54, doi:10.1111/j.1469-8137.2007.02062.x.
- APA
- Vandeputte, O., Vereecke, D., Mol, A., Lenjou, M., Van Bockstaele, D., El Jaziri, M., & Baucher, M. (2007). Rhodococcus fascians infection accelerates progression of tobacco BY-2 cells into mitosis through rapid changes in plant gene expression. NEW PHYTOLOGIST, 175(1), 140–154. https://doi.org/10.1111/j.1469-8137.2007.02062.x
- Chicago author-date
- Vandeputte, Olivier, Danny Vereecke, Adeline Mol, Marc Lenjou, Dirk Van Bockstaele, Mondher El Jaziri, and Marie Baucher. 2007. “Rhodococcus Fascians Infection Accelerates Progression of Tobacco BY-2 Cells into Mitosis through Rapid Changes in Plant Gene Expression.” NEW PHYTOLOGIST 175 (1): 140–54. https://doi.org/10.1111/j.1469-8137.2007.02062.x.
- Chicago author-date (all authors)
- Vandeputte, Olivier, Danny Vereecke, Adeline Mol, Marc Lenjou, Dirk Van Bockstaele, Mondher El Jaziri, and Marie Baucher. 2007. “Rhodococcus Fascians Infection Accelerates Progression of Tobacco BY-2 Cells into Mitosis through Rapid Changes in Plant Gene Expression.” NEW PHYTOLOGIST 175 (1): 140–154. doi:10.1111/j.1469-8137.2007.02062.x.
- Vancouver
- 1.Vandeputte O, Vereecke D, Mol A, Lenjou M, Van Bockstaele D, El Jaziri M, et al. Rhodococcus fascians infection accelerates progression of tobacco BY-2 cells into mitosis through rapid changes in plant gene expression. NEW PHYTOLOGIST. 2007;175(1):140–54.
- IEEE
- [1]O. Vandeputte et al., “Rhodococcus fascians infection accelerates progression of tobacco BY-2 cells into mitosis through rapid changes in plant gene expression,” NEW PHYTOLOGIST, vol. 175, no. 1, pp. 140–154, 2007.
@article{409134,
abstract = {{To characterize plant cell cycle activation following Rhodococcus fascians infection, bacterial impact on cell cycle progression of tobacco BY-2 cells was investigated.
S-phase-synchronized BY-2 cells were cocultivated with R. fascians and cell cycle progression was monitored by measuring mitotic index, cell cycle gene expression and flow cytometry parameters. Cell cycle alteration was further investigated by cDNA-AFLP (amplified fragment length polymorphism).
It was shown that cell cycle progression of BY-2 cells was accelerated only upon infection with bacteria whose virulence gene expression was induced by a leafy gall extract. Thirty-eight BY-2 genes showed a differential expression within 6 h post-infection. Among these, seven were previously associated with specific plant cell cycle phases (in particular S and G2/M phases). Several genes also showed a differential expression during leafy gall formation.
R. fascians-infected BY-2 cells provide a simple model to identify plant genes related to leafy gall development. R. fascians can also be regarded as a useful biotic agent to alter cell cycle progression and, thereby, gain a better understanding of cell cycle regulation in plants.}},
author = {{Vandeputte, Olivier and Vereecke, Danny and Mol, Adeline and Lenjou, Marc and Van Bockstaele, Dirk and El Jaziri, Mondher and Baucher, Marie}},
issn = {{0028-646X}},
journal = {{NEW PHYTOLOGIST}},
keywords = {{PROTEIN,REAL-TIME,TRANSITION,TOPOLOGY PREDICTION,ABSCISIC-ACID,S-PHASE,DIFFERENTIAL REGULATION,D-TYPE CYCLIN,LEAFY GALL FORMATION,ZEATIN TYPE CYTOKININS,Rhodococcus fascians,plant-bacteria interactions,leafy gall,hyperplasia,cell cycle,BY-2,cDNA-AFLP (amplified fragment length polymorphism)}},
language = {{eng}},
number = {{1}},
pages = {{140--154}},
title = {{Rhodococcus fascians infection accelerates progression of tobacco BY-2 cells into mitosis through rapid changes in plant gene expression}},
url = {{http://doi.org/10.1111/j.1469-8137.2007.02062.x}},
volume = {{175}},
year = {{2007}},
}
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