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A symbiotic plant peroxidase involved in bacterial invasion of the tropical legume Sesbania rostrata

Jeroen Den Herder (UGent) , Sam Lievens (UGent) , Stephane Rombauts (UGent) , Marcella Holsters (UGent) and Sofie Goormachtig (UGent)
(2007) PLANT PHYSIOLOGY. 144(2). p.717-727
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Abstract
Aquatic nodulation on the tropical legume Sesbania rostrata occurs at lateral root bases via intercellular crack-entry invasion. A gene was identified (Srprx1) that is transiently up-regulated during the nodulation process and codes for a functional class III plant peroxidase. The expression strictly depended on bacterial nodulation factors (NFs) and could be modulated by hydrogen peroxide, a downstream signal for crack-entry invasion. Expression was not induced after wounding or pathogen attack, indicating that the peroxidase is a symbiosis-specific isoform. In situ hybridization showed Srprx1 transcripts around bacterial infection pockets and infection threads until they reached the central tissue of the nodule. A root nodule extensin (SrRNE1) colocalized with Srprx1 both in time and space and had the same NF requirement, suggesting a function in a similar process. Finally, in mixed inoculation nodules that were invaded by NF-deficient bacteria and differed in infection thread progression, infection-associated peroxidase transcripts were not observed. Lack of Srprx1 gene expression could be one of the causes for the aberrant structure of the infection threads.
Keywords
ARABIDOPSIS-THALIANA, MEDICAGO-TRUNCATULA, PEA ROOT-NODULES, NOD FACTOR PERCEPTION, AZORHIZOBIUM-CAULINODANS, SINORHIZOBIUM-MELILOTI, GENE FAMILY, DIFFERENTIAL REGULATION, SUPEROXIDE-DISMUTASE, INTERCELLULAR INVASION

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Citation

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Chicago
Den Herder, Jeroen, Sam Lievens, Stephane Rombauts, Marcella Holsters, and Sofie Goormachtig. 2007. “A Symbiotic Plant Peroxidase Involved in Bacterial Invasion of the Tropical Legume Sesbania Rostrata.” Plant Physiology 144 (2): 717–727.
APA
Den Herder, J., Lievens, S., Rombauts, S., Holsters, M., & Goormachtig, S. (2007). A symbiotic plant peroxidase involved in bacterial invasion of the tropical legume Sesbania rostrata. PLANT PHYSIOLOGY, 144(2), 717–727.
Vancouver
1.
Den Herder J, Lievens S, Rombauts S, Holsters M, Goormachtig S. A symbiotic plant peroxidase involved in bacterial invasion of the tropical legume Sesbania rostrata. PLANT PHYSIOLOGY. 2007;144(2):717–27.
MLA
Den Herder, Jeroen, Sam Lievens, Stephane Rombauts, et al. “A Symbiotic Plant Peroxidase Involved in Bacterial Invasion of the Tropical Legume Sesbania Rostrata.” PLANT PHYSIOLOGY 144.2 (2007): 717–727. Print.
@article{409132,
  abstract     = {Aquatic nodulation on the tropical legume Sesbania rostrata occurs at lateral root bases via intercellular crack-entry invasion. A gene was identified (Srprx1) that is transiently up-regulated during the nodulation process and codes for a functional class III plant peroxidase. The expression strictly depended on bacterial nodulation factors (NFs) and could be modulated by hydrogen peroxide, a downstream signal for crack-entry invasion. Expression was not induced after wounding or pathogen attack, indicating that the peroxidase is a symbiosis-specific isoform. In situ hybridization showed Srprx1 transcripts around bacterial infection pockets and infection threads until they reached the central tissue of the nodule. A root nodule extensin (SrRNE1) colocalized with Srprx1 both in time and space and had the same NF requirement, suggesting a function in a similar process. Finally, in mixed inoculation nodules that were invaded by NF-deficient bacteria and differed in infection thread progression, infection-associated peroxidase transcripts were not observed. Lack of Srprx1 gene expression could be one of the causes for the aberrant structure of the infection threads.},
  author       = {Den Herder, Jeroen and Lievens, Sam and Rombauts, Stephane and Holsters, Marcella and Goormachtig, Sofie},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {ARABIDOPSIS-THALIANA,MEDICAGO-TRUNCATULA,PEA ROOT-NODULES,NOD FACTOR PERCEPTION,AZORHIZOBIUM-CAULINODANS,SINORHIZOBIUM-MELILOTI,GENE FAMILY,DIFFERENTIAL REGULATION,SUPEROXIDE-DISMUTASE,INTERCELLULAR INVASION},
  language     = {eng},
  number       = {2},
  pages        = {717--727},
  title        = {A symbiotic plant peroxidase involved in bacterial invasion of the tropical legume Sesbania rostrata},
  url          = {http://dx.doi.org/10.1104/pp.107.098764},
  volume       = {144},
  year         = {2007},
}

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