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Hydrogen peroxide-induced gene expression across kingdoms: a comparative analysis

Korneel Vandenbroucke (UGent) , Steven Robbens (UGent) , Klaas Vandepoele (UGent) , Dirk Inzé (UGent) , Yves Van de Peer (UGent) and Frank Van Breusegem (UGent)
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Abstract
Cells react to oxidative stress conditions by launching a defense response through the induction of nuclear gene expression. The advent of microarray technologies allowed monitoring of oxidative stress-dependent changes of transcript levels at a comprehensive and genome-wide scale, resulting in a series of inventories of differentially expressed genes in different organisms. We performed a meta-analysis on hydrogen peroxide (H2O2)-induced gene expression in the cyanobacterium Synechocystis PCC 6803, the yeast Saccharomyces cerevisiae and Schizosaccharomyces pombe, the land plant Arabidopsis thaliana, and the human HeLa cell line. The H2O2-induced gene expression in both yeast species was highly conserved and more similar to the A. thaliana response than that of the human cell line. Based on the expression characteristics of genuine antioxidant genes, we show that the antioxidant capacity of microorganisms and higher eukaryotes is differentially regulated. Four families of evolutionarily conserved eukaryotic proteins could be identified that were H2O2 responsive across kingdoms: DNAJ domain-containing heat shock proteins, small guanine triphosphate-binding proteins, Ca2+-dependent protein kinases, and ubiquitin-conjugating enzymes.
Keywords
REDOX REGULATION, ARABIDOPSIS GENOME, TRANSCRIPTION FACTOR, CELL-DEATH, REACTIVE OXYGEN, OXIDATIVE STRESS, HEAT-SHOCK-PROTEIN, DNA-BINDING ACTIVITY, CYTOSOLIC ASCORBATE PEROXIDASE, H. sapiens, Arabidopsis, yeast, Synechocystis, comparative transcriptomics, microarray, oxidative stress, hydrogen peroxide, SACCHAROMYCES-CEREVISIAE

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Chicago
Vandenbroucke, Korneel, Steven Robbens, Klaas Vandepoele, Dirk Inzé, Yves Van de Peer, and Frank Van Breusegem. 2008. “Hydrogen Peroxide-induced Gene Expression Across Kingdoms: a Comparative Analysis.” Molecular Biology and Evolution 25 (3): 507–516.
APA
Vandenbroucke, Korneel, Robbens, S., Vandepoele, K., Inzé, D., Van de Peer, Y., & Van Breusegem, F. (2008). Hydrogen peroxide-induced gene expression across kingdoms: a comparative analysis. MOLECULAR BIOLOGY AND EVOLUTION, 25(3), 507–516.
Vancouver
1.
Vandenbroucke K, Robbens S, Vandepoele K, Inzé D, Van de Peer Y, Van Breusegem F. Hydrogen peroxide-induced gene expression across kingdoms: a comparative analysis. MOLECULAR BIOLOGY AND EVOLUTION. 2008;25(3):507–16.
MLA
Vandenbroucke, Korneel, Steven Robbens, Klaas Vandepoele, et al. “Hydrogen Peroxide-induced Gene Expression Across Kingdoms: a Comparative Analysis.” MOLECULAR BIOLOGY AND EVOLUTION 25.3 (2008): 507–516. Print.
@article{409114,
  abstract     = {Cells react to oxidative stress conditions by launching a defense response through the induction of nuclear gene expression. The advent of microarray technologies allowed monitoring of oxidative stress-dependent changes of transcript levels at a comprehensive and genome-wide scale, resulting in a series of inventories of differentially expressed genes in different organisms. We performed a meta-analysis on hydrogen peroxide (H2O2)-induced gene expression in the cyanobacterium Synechocystis PCC 6803, the yeast Saccharomyces cerevisiae and Schizosaccharomyces pombe, the land plant Arabidopsis thaliana, and the human HeLa cell line. The H2O2-induced gene expression in both yeast species was highly conserved and more similar to the A. thaliana response than that of the human cell line. Based on the expression characteristics of genuine antioxidant genes, we show that the antioxidant capacity of microorganisms and higher eukaryotes is differentially regulated. Four families of evolutionarily conserved eukaryotic proteins could be identified that were H2O2 responsive across kingdoms: DNAJ domain-containing heat shock proteins, small guanine triphosphate-binding proteins, Ca2+-dependent protein kinases, and ubiquitin-conjugating enzymes.},
  author       = {Vandenbroucke, Korneel and Robbens, Steven and Vandepoele, Klaas and Inz{\'e}, Dirk and Van de Peer, Yves and Van Breusegem, Frank},
  issn         = {0737-4038},
  journal      = {MOLECULAR BIOLOGY AND EVOLUTION},
  language     = {eng},
  number       = {3},
  pages        = {507--516},
  title        = {Hydrogen peroxide-induced gene expression across kingdoms: a comparative analysis},
  url          = {http://dx.doi.org/10.1093/molbev/msm276},
  volume       = {25},
  year         = {2008},
}

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