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Arabidopsis WEE1 kinase controls cell cycle arrest in response to activation of the DNA integrity checkpoint

Kristof De Schutter UGent, Jérôme Joubès UGent, Toon Cools UGent, Aurine Verkest UGent, Florence Corellou, Elena Babiychuk UGent, ELS VAN DER SCHUEREN UGent, Tom Beeckman UGent, Sergei Kushnir UGent and Dirk Inzé UGent, et al. (2007) PLANT CELL. 19(1). p.211-225
abstract
Upon the incidence of DNA stress, the ataxia telangiectasia-mutated (ATM) and Rad3-related(ATR) signaling kinases activate a transient cell cycle arrest that allows cells to repair DNA before proceeding into mitosis. Although the ATM-ATRpathway is highly conserved over species, the mechanisms by which plant cells stop their cell cycle in response to the loss of genome integrity are unclear. We demonstrate that the cell cycle regulatory WEE1 kinase gene of Arabidopsis thaliana is transcriptionally activated upon the cessation of DNA replication or DNA damage in an ATR- or ATM-dependent manner, respectively. In accordance with a role for WEE1 in DNA stress signaling, WEE1-deficient plants showed no obvious cell division or endoreduplication phenotype when grown under nonstress conditions but were hypersensitive to agents that impair DNA replication. Induced WEE1 expression inhibited plant growth by arresting dividing cells in the G2-phase of the cell cycle. We conclude that the plant WEE1 gene is not rate-limiting for cycle progression under normal growth conditions but is a critical target of the ATR-ATM signaling cascades that inhibit the cell cycle upon activation of the DNA integrity checkpoints, coupling mitosis to DNA repair in cells that suffer DNA damage.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CDC25 PHOSPHATASE, TRANSCRIPTION FACTOR, MEDIATED TRANSFORMATION, SCHIZOSACCHAROMYCES-POMBE, DAMAGE CHECKPOINT, GENOME-WIDE ANALYSIS, DEPENDENT KINASE, PLANT DEVELOPMENT, S PHASE, GENOTOXIC STRESS
journal title
PLANT CELL
Plant Cell
volume
19
issue
1
pages
211 - 225
Web of Science type
Article
Web of Science id
000244757400019
JCR category
PLANT SCIENCES
JCR impact factor
9.653 (2007)
JCR rank
3/149 (2007)
JCR quartile
1 (2007)
ISSN
1040-4651
DOI
10.1105/tpc.106.045047
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
409102
handle
http://hdl.handle.net/1854/LU-409102
date created
2008-05-15 15:03:00
date last changed
2013-10-11 10:43:25
@article{409102,
  abstract     = {Upon the incidence of DNA stress, the ataxia telangiectasia-mutated (ATM) and Rad3-related(ATR) signaling kinases activate a transient cell cycle arrest that allows cells to repair DNA before proceeding into mitosis. Although the ATM-ATRpathway is highly conserved over species, the mechanisms by which plant cells stop their cell cycle in response to the loss of genome integrity are unclear. We demonstrate that the cell cycle regulatory WEE1 kinase gene of Arabidopsis thaliana is transcriptionally activated upon the cessation of DNA replication or DNA damage in an ATR- or ATM-dependent manner, respectively. In accordance with a role for WEE1 in DNA stress signaling, WEE1-deficient plants showed no obvious cell division or endoreduplication phenotype when grown under nonstress conditions but were hypersensitive to agents that impair DNA replication. Induced WEE1 expression inhibited plant growth by arresting dividing cells in the G2-phase of the cell cycle. We conclude that the plant WEE1 gene is not rate-limiting for cycle progression under normal growth conditions but is a critical target of the ATR-ATM signaling cascades that inhibit the cell cycle upon activation of the DNA integrity checkpoints, coupling mitosis to DNA repair in cells that suffer DNA damage.},
  author       = {De Schutter, Kristof and Joub{\`e}s, J{\'e}r{\^o}me and Cools, Toon and Verkest, Aurine and Corellou, Florence and Babiychuk, Elena and VAN DER SCHUEREN, ELS and Beeckman, Tom and Kushnir, Sergei and Inz{\'e}, Dirk and De Veylder, Lieven},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {CDC25 PHOSPHATASE,TRANSCRIPTION FACTOR,MEDIATED TRANSFORMATION,SCHIZOSACCHAROMYCES-POMBE,DAMAGE CHECKPOINT,GENOME-WIDE ANALYSIS,DEPENDENT KINASE,PLANT DEVELOPMENT,S PHASE,GENOTOXIC STRESS},
  language     = {eng},
  number       = {1},
  pages        = {211--225},
  title        = {Arabidopsis WEE1 kinase controls cell cycle arrest in response to activation of the DNA integrity checkpoint},
  url          = {http://dx.doi.org/10.1105/tpc.106.045047},
  volume       = {19},
  year         = {2007},
}

Chicago
De Schutter, Kristof, Jérôme Joubès, Toon Cools, Aurine Verkest, Florence Corellou, Elena Babiychuk, ELS VAN DER SCHUEREN, et al. 2007. “Arabidopsis WEE1 Kinase Controls Cell Cycle Arrest in Response to Activation of the DNA Integrity Checkpoint.” Plant Cell 19 (1): 211–225.
APA
De Schutter, Kristof, Joubès, J., Cools, T., Verkest, A., Corellou, F., Babiychuk, E., VAN DER SCHUEREN, E., et al. (2007). Arabidopsis WEE1 kinase controls cell cycle arrest in response to activation of the DNA integrity checkpoint. PLANT CELL, 19(1), 211–225.
Vancouver
1.
De Schutter K, Joubès J, Cools T, Verkest A, Corellou F, Babiychuk E, et al. Arabidopsis WEE1 kinase controls cell cycle arrest in response to activation of the DNA integrity checkpoint. PLANT CELL. 2007;19(1):211–25.
MLA
De Schutter, Kristof, Jérôme Joubès, Toon Cools, et al. “Arabidopsis WEE1 Kinase Controls Cell Cycle Arrest in Response to Activation of the DNA Integrity Checkpoint.” PLANT CELL 19.1 (2007): 211–225. Print.