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A qualitative comparison of 3D visualization in Xenopus laevis using a traditional method and a non-destructive method

Emilie Descamps, Jan Buytaert, Barbara De Kegel UGent, Joris Dirckx and Dominique Adriaens UGent (2012) BELGIAN JOURNAL OF ZOOLOGY. 142(2). p.99-111
abstract
Many tools are currently available to investigate and visualize soft and hard tissues in animals both in high-resolution and three dimensions. The most popular and traditional method is based on destructive histological techniques. However, these techniques have some specific limitations. In order to avoid those limitations, various non-destructive approaches have surfaced in the last decades. One of those is micro-CT-scanning. In the best conditions, resolution achieved in micro-CT currently approaches that of standard histological protocols. In addition to bone, soft tissues can also be made visible through micro-CT-scanning. However, discriminating between structures of the same tissue and among different tissue types remains a challenge. An alternative approach, which has not yet been explored to its full potential for comparative anatomy studies, is Orthogonal-Plane Fluorescence Optical Sectioning (OPFOS) microscopy or tomography, also known as (Laser) Light Sheet based Fluorescence Microscopy (LSFM). In this study, we compare OPFOS with light microscopy, applying those techniques to the model organism Xenopus laevis. The potential of both methods for discrimination between different types of tissues, as well as different structures of the same tissue type, is tested and illustrated. Since the histological sections provided a better resolution, adjacent structures of the same tissue type could be discerned more easily compared to our OPFOS images. However, we obtained a more naturally-shaped 3D model of the musculoskeletal system of Xenopus laevis with OPFOS. An overview of the advantages and disadvantages of both techniques is given and their applicability for a wider scope of biological research is discussed.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
histological sectioning, vertebrates, optical sectioning, Xenopus, 3D visualization, MAGNETIC-RESONANCE MICROSCOPY, GRADIENT-ECHO SEQUENCE, 3-DIMENSIONAL RECONSTRUCTION, HIGH-RESOLUTION, MORPHOLOGY, TOMOGRAPHY, SPECIMENS, EMBRYOS, AUTOFLUORESCENCE, ULTRAMICROSCOPY
journal title
BELGIAN JOURNAL OF ZOOLOGY
Belg. J. Zool.
volume
142
issue
2
pages
99 - 111
Web of Science type
Article
Web of Science id
000312578600002
JCR category
ZOOLOGY
JCR impact factor
0.702 (2012)
JCR rank
106/149 (2012)
JCR quartile
3 (2012)
ISSN
0777-6276
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4088661
handle
http://hdl.handle.net/1854/LU-4088661
date created
2013-06-27 14:54:09
date last changed
2018-04-11 10:07:53
@article{4088661,
  abstract     = {Many tools are currently available to investigate and visualize soft and hard tissues in animals both in high-resolution and three dimensions. The most popular and traditional method is based on destructive histological techniques. However, these techniques have some specific limitations. In order to avoid those limitations, various non-destructive approaches have surfaced in the last decades. One of those is micro-CT-scanning. In the best conditions, resolution achieved in micro-CT currently approaches that of standard histological protocols. In addition to bone, soft tissues can also be made visible through micro-CT-scanning. However, discriminating between structures of the same tissue and among different tissue types remains a challenge. An alternative approach, which has not yet been explored to its full potential for comparative anatomy studies, is Orthogonal-Plane Fluorescence Optical Sectioning (OPFOS) microscopy or tomography, also known as (Laser) Light Sheet based Fluorescence Microscopy (LSFM). In this study, we compare OPFOS with light microscopy, applying those techniques to the model organism Xenopus laevis. The potential of both methods for discrimination between different types of tissues, as well as different structures of the same tissue type, is tested and illustrated. Since the histological sections provided a better resolution, adjacent structures of the same tissue type could be discerned more easily compared to our OPFOS images. However, we obtained a more naturally-shaped 3D model of the musculoskeletal system of Xenopus laevis with OPFOS. An overview of the advantages and disadvantages of both techniques is given and their applicability for a wider scope of biological research is discussed.},
  author       = {Descamps, Emilie and Buytaert, Jan and De Kegel, Barbara and Dirckx, Joris and Adriaens, Dominique},
  issn         = {0777-6276},
  journal      = {BELGIAN JOURNAL OF ZOOLOGY},
  keyword      = {histological sectioning,vertebrates,optical sectioning,Xenopus,3D visualization,MAGNETIC-RESONANCE MICROSCOPY,GRADIENT-ECHO SEQUENCE,3-DIMENSIONAL RECONSTRUCTION,HIGH-RESOLUTION,MORPHOLOGY,TOMOGRAPHY,SPECIMENS,EMBRYOS,AUTOFLUORESCENCE,ULTRAMICROSCOPY},
  language     = {eng},
  number       = {2},
  pages        = {99--111},
  title        = {A qualitative comparison of 3D visualization in Xenopus laevis using a traditional method and a non-destructive method},
  volume       = {142},
  year         = {2012},
}

Chicago
Descamps, Emilie, Jan Buytaert, Barbara De Kegel, Joris Dirckx, and Dominique Adriaens. 2012. “A Qualitative Comparison of 3D Visualization in Xenopus Laevis Using a Traditional Method and a Non-destructive Method.” Belgian Journal of Zoology 142 (2): 99–111.
APA
Descamps, E., Buytaert, J., De Kegel, B., Dirckx, J., & Adriaens, D. (2012). A qualitative comparison of 3D visualization in Xenopus laevis using a traditional method and a non-destructive method. BELGIAN JOURNAL OF ZOOLOGY, 142(2), 99–111.
Vancouver
1.
Descamps E, Buytaert J, De Kegel B, Dirckx J, Adriaens D. A qualitative comparison of 3D visualization in Xenopus laevis using a traditional method and a non-destructive method. BELGIAN JOURNAL OF ZOOLOGY. 2012;142(2):99–111.
MLA
Descamps, Emilie, Jan Buytaert, Barbara De Kegel, et al. “A Qualitative Comparison of 3D Visualization in Xenopus Laevis Using a Traditional Method and a Non-destructive Method.” BELGIAN JOURNAL OF ZOOLOGY 142.2 (2012): 99–111. Print.