Ghent University Academic Bibliography

Advanced

A tandem affinity purification-based technology platform to study the cell cycle interactome in Arabidopsis thaliana

Jelle Van Leene UGent, Hilde Stals UGent, Dominique Eeckhout UGent, Geert Persiau UGent, Eveline Van De Slijke UGent, Gert Van Isterdael UGent, Annelies De Clercq UGent, Eric Bonnet UGent, Kris Laukens and Noor Remmerie, et al. (2007) MOLECULAR & CELLULAR PROTEOMICS. 6(7). p.1226-1238
abstract
Defining protein complexes is critical to virtually all aspects of cell biology because many cellular processes are regulated by stable protein complexes, and their identification often provides insights into their function. We describe the development and application of a high throughput tandem affinity purification/mass spectrometry platform for cell suspension cultures to analyze cell cycle-related protein complexes in Arabidopsis thaliana. Elucidation of this protein-protein interaction network is essential to fully understand the functional differences between the highly redundant cyclin-dependent kinase/cyclin modules, which are generally accepted to play a central role in cell cycle control, in all eukaryotes. Cell suspension cultures were chosen because they provide an unlimited supply of protein extracts of actively dividing and undifferentiated cells, which is crucial for a systematic study of the cell cycle interactome in the absence of plant development. Here we report the mapping of a protein interaction network around six known core cell cycle proteins by an integrated approach comprising generic Gateway-based vectors with high cloning flexibility, the fast generation of transgenic suspension cultures, tandem affinity purification adapted for plant cells, matrix- assisted laser desorption ionization tandem mass spectrometry, data analysis, and functional assays. We identified 28 new molecular associations and confirmed 14 previously described interactions. This systemic approach provides new insights into the basic cell cycle control mechanisms and is generally applicable to other pathways in plants.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
YEAST, IN-VIVO, S-PHASE, PROTEIN COMPLEXES, ACTIVATING KINASES, DEPENDENT KINASE INHIBITORS, SACCHAROMYCES-CEREVISIAE, MASS-SPECTROMETRY, INTERACTION NETWORKS, SUSPENSION-CULTURES
journal title
MOLECULAR & CELLULAR PROTEOMICS
Mol. Cell. Proteomics
volume
6
issue
7
pages
1226 - 1238
Web of Science type
Article
Web of Science id
000247850200011
JCR category
BIOCHEMICAL RESEARCH METHODS
JCR impact factor
9.425 (2007)
JCR rank
2/57 (2007)
JCR quartile
1 (2007)
ISSN
1535-9476
DOI
10.1074/mcp.M700078-MCP200
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
408354
handle
http://hdl.handle.net/1854/LU-408354
date created
2008-05-14 17:32:00
date last changed
2013-10-17 16:02:01
@article{408354,
  abstract     = {Defining protein complexes is critical to virtually all aspects of cell biology because many cellular processes are regulated by stable protein complexes, and their identification often provides insights into their function. We describe the development and application of a high throughput tandem affinity purification/mass spectrometry platform for cell suspension cultures to analyze cell cycle-related protein complexes in Arabidopsis thaliana. Elucidation of this protein-protein interaction network is essential to fully understand the functional differences between the highly redundant cyclin-dependent kinase/cyclin modules, which are generally accepted to play a central role in cell cycle control, in all eukaryotes. Cell suspension cultures were chosen because they provide an unlimited supply of protein extracts of actively dividing and undifferentiated cells, which is crucial for a systematic study of the cell cycle interactome in the absence of plant development. Here we report the mapping of a protein interaction network around six known core cell cycle proteins by an integrated approach comprising generic Gateway-based vectors with high cloning flexibility, the fast generation of transgenic suspension cultures, tandem affinity purification adapted for plant cells, matrix- assisted laser desorption ionization tandem mass spectrometry, data analysis, and functional assays. We identified 28 new molecular associations and confirmed 14 previously described interactions. This systemic approach provides new insights into the basic cell cycle control mechanisms and is generally applicable to other pathways in plants.},
  author       = {Van Leene, Jelle and Stals, Hilde and Eeckhout, Dominique and Persiau, Geert and Van De Slijke, Eveline and Van Isterdael, Gert and De Clercq, Annelies and Bonnet, Eric and Laukens, Kris and Remmerie, Noor and Henderickx, Kim and De Vijlder, Thomas and Abdelkrim, Azmi and Pharazyn, Anne and Van Onckelen, Harry and Inz{\'e}, Dirk and Witters, Erwin and De Jaeger, Geert},
  issn         = {1535-9476},
  journal      = {MOLECULAR \& CELLULAR PROTEOMICS},
  keyword      = {YEAST,IN-VIVO,S-PHASE,PROTEIN COMPLEXES,ACTIVATING KINASES,DEPENDENT KINASE INHIBITORS,SACCHAROMYCES-CEREVISIAE,MASS-SPECTROMETRY,INTERACTION NETWORKS,SUSPENSION-CULTURES},
  language     = {eng},
  number       = {7},
  pages        = {1226--1238},
  title        = {A tandem affinity purification-based technology platform to study the cell cycle interactome in Arabidopsis thaliana},
  url          = {http://dx.doi.org/10.1074/mcp.M700078-MCP200},
  volume       = {6},
  year         = {2007},
}

Chicago
Van Leene, Jelle, Hilde Stals, Dominique Eeckhout, Geert Persiau, Eveline Van De Slijke, Gert Van Isterdael, Annelies De Clercq, et al. 2007. “A Tandem Affinity Purification-based Technology Platform to Study the Cell Cycle Interactome in Arabidopsis Thaliana.” Molecular & Cellular Proteomics 6 (7): 1226–1238.
APA
Van Leene, J., Stals, H., Eeckhout, D., Persiau, G., Van De Slijke, E., Van Isterdael, G., De Clercq, A., et al. (2007). A tandem affinity purification-based technology platform to study the cell cycle interactome in Arabidopsis thaliana. MOLECULAR & CELLULAR PROTEOMICS, 6(7), 1226–1238.
Vancouver
1.
Van Leene J, Stals H, Eeckhout D, Persiau G, Van De Slijke E, Van Isterdael G, et al. A tandem affinity purification-based technology platform to study the cell cycle interactome in Arabidopsis thaliana. MOLECULAR & CELLULAR PROTEOMICS. 2007;6(7):1226–38.
MLA
Van Leene, Jelle, Hilde Stals, Dominique Eeckhout, et al. “A Tandem Affinity Purification-based Technology Platform to Study the Cell Cycle Interactome in Arabidopsis Thaliana.” MOLECULAR & CELLULAR PROTEOMICS 6.7 (2007): 1226–1238. Print.