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A temperature-sensitive mutation in the Arabidopsis thaliana phosphomannomutase gene disrupts protein glycosylation and triggers cell death

Frank Hoeberichts UGent, Olivier Van Aken UGent, Guy Kiddle, Emmy Coppens, Brigitte Van De Cotte UGent, Antoine Adamantidis, Sandra Ormenese, Christine H Foyer, Marc Zabeau, Dirk Inzé UGent, et al. (2008) JOURNAL OF BIOLOGICAL CHEMISTRY. 283(9). p.5708-5718
abstract
Eukaryotic phosphomannomutases (PMMs) catalyze the interconversion of mannose 6-phosphate to mannose 1-phosphate and are essential to the biosynthesis of GDP-mannose. As such, plant PMMs are involved in ascorbic acid (AsA) biosynthesis and N-glycosylation. We report on the conditional phenotype of the temperature-sensitive Arabidopsis thaliana pmm- 12 mutant. Mutant seedlings were phenotypically similar to wild type seedlings when grown at 16 - 18 degrees C but died within several days after transfer to 28 degrees C. This phenotype was observed throughout both vegetative and reproductive development. Protein extracts derived from pmm-12 plants had lower PMM protein and enzyme activity levels. In vitro biochemical analysis of recombinant proteins showed that the mutant PMM protein was compromised in its catalytic efficiency (K-cat/K-m). Despite significantly decreased AsA levels in pmm-12 plants, AsA deficiency could not account for the observed phenotype. Since, at restrictive temperature, total glycoprotein patterns were altered and glycosylation of protein-disulfide isomerase was perturbed, we propose that a deficiency in protein glycosylation is responsible for the observed cell death phenotype.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
L-ASCORBIC-ACID, CELLULOSE BIOSYNTHESIS, DISULFIDE-ISOMERASE, GLUCOSIDASE-I, HIGHER-PLANTS, GDP-MANNOSE, VITAMIN-C, DEFICIENT, MUTANTS, OLIGOSACCHARYLTRANSFERASE
journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
J. Biol. Chem.
volume
283
issue
9
pages
5708 - 5718
Web of Science type
Article
Web of Science id
000253426700052
JCR category
BIOCHEMISTRY & MOLECULAR BIOLOGY
JCR impact factor
5.52 (2008)
JCR rank
47/276 (2008)
JCR quartile
1 (2008)
ISSN
0021-9258
DOI
10.1074/jbc.M704991200
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
408338
handle
http://hdl.handle.net/1854/LU-408338
date created
2008-05-14 16:45:00
date last changed
2016-12-19 15:43:05
@article{408338,
  abstract     = {Eukaryotic phosphomannomutases (PMMs) catalyze the interconversion of mannose 6-phosphate to mannose 1-phosphate and are essential to the biosynthesis of GDP-mannose. As such, plant PMMs are involved in ascorbic acid (AsA) biosynthesis and N-glycosylation. We report on the conditional phenotype of the temperature-sensitive Arabidopsis thaliana pmm- 12 mutant. Mutant seedlings were phenotypically similar to wild type seedlings when grown at 16 - 18 degrees C but died within several days after transfer to 28 degrees C. This phenotype was observed throughout both vegetative and reproductive development. Protein extracts derived from pmm-12 plants had lower PMM protein and enzyme activity levels. In vitro biochemical analysis of recombinant proteins showed that the mutant PMM protein was compromised in its catalytic efficiency (K-cat/K-m). Despite significantly decreased AsA levels in pmm-12 plants, AsA deficiency could not account for the observed phenotype. Since, at restrictive temperature, total glycoprotein patterns were altered and glycosylation of protein-disulfide isomerase was perturbed, we propose that a deficiency in protein glycosylation is responsible for the observed cell death phenotype.},
  author       = {Hoeberichts, Frank and Van Aken, Olivier and Kiddle, Guy and Coppens, Emmy and Van De Cotte, Brigitte and Adamantidis, Antoine and Ormenese, Sandra and Foyer, Christine H and Zabeau, Marc and Inz{\'e}, Dirk and P{\'e}rilleux, Claire and Van Breusegem, Frank and Vuylsteke, Marnik},
  issn         = {0021-9258},
  journal      = {JOURNAL OF BIOLOGICAL CHEMISTRY},
  keyword      = {L-ASCORBIC-ACID,CELLULOSE BIOSYNTHESIS,DISULFIDE-ISOMERASE,GLUCOSIDASE-I,HIGHER-PLANTS,GDP-MANNOSE,VITAMIN-C,DEFICIENT,MUTANTS,OLIGOSACCHARYLTRANSFERASE},
  language     = {eng},
  number       = {9},
  pages        = {5708--5718},
  title        = {A temperature-sensitive mutation in the Arabidopsis thaliana phosphomannomutase gene disrupts protein glycosylation and triggers cell death},
  url          = {http://dx.doi.org/10.1074/jbc.M704991200},
  volume       = {283},
  year         = {2008},
}

Chicago
Hoeberichts, Frank, Elke Vaeck, Guy Kiddle, Emmy Coppens, Brigitte Van De Cotte, Antoine Adamantidis, Sandra Ormenese, et al. 2008. “A Temperature-sensitive Mutation in the Arabidopsis Thaliana Phosphomannomutase Gene Disrupts Protein Glycosylation and Triggers Cell Death.” Journal of Biological Chemistry 283 (9): 5708–5718.
APA
Hoeberichts, F., Vaeck, E., Kiddle, G., Coppens, E., Van De Cotte, B., Adamantidis, A., Ormenese, S., et al. (2008). A temperature-sensitive mutation in the Arabidopsis thaliana phosphomannomutase gene disrupts protein glycosylation and triggers cell death. JOURNAL OF BIOLOGICAL CHEMISTRY, 283(9), 5708–5718.
Vancouver
1.
Hoeberichts F, Vaeck E, Kiddle G, Coppens E, Van De Cotte B, Adamantidis A, et al. A temperature-sensitive mutation in the Arabidopsis thaliana phosphomannomutase gene disrupts protein glycosylation and triggers cell death. JOURNAL OF BIOLOGICAL CHEMISTRY. 2008;283(9):5708–18.
MLA
Hoeberichts, Frank, Elke Vaeck, Guy Kiddle, et al. “A Temperature-sensitive Mutation in the Arabidopsis Thaliana Phosphomannomutase Gene Disrupts Protein Glycosylation and Triggers Cell Death.” JOURNAL OF BIOLOGICAL CHEMISTRY 283.9 (2008): 5708–5718. Print.