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A temperature-sensitive mutation in the Arabidopsis thaliana phosphomannomutase gene disrupts protein glycosylation and triggers cell death

(2008) JOURNAL OF BIOLOGICAL CHEMISTRY. 283(9). p.5708-5718
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Abstract
Eukaryotic phosphomannomutases (PMMs) catalyze the interconversion of mannose 6-phosphate to mannose 1-phosphate and are essential to the biosynthesis of GDP-mannose. As such, plant PMMs are involved in ascorbic acid (AsA) biosynthesis and N-glycosylation. We report on the conditional phenotype of the temperature-sensitive Arabidopsis thaliana pmm- 12 mutant. Mutant seedlings were phenotypically similar to wild type seedlings when grown at 16 - 18 degrees C but died within several days after transfer to 28 degrees C. This phenotype was observed throughout both vegetative and reproductive development. Protein extracts derived from pmm-12 plants had lower PMM protein and enzyme activity levels. In vitro biochemical analysis of recombinant proteins showed that the mutant PMM protein was compromised in its catalytic efficiency (K-cat/K-m). Despite significantly decreased AsA levels in pmm-12 plants, AsA deficiency could not account for the observed phenotype. Since, at restrictive temperature, total glycoprotein patterns were altered and glycosylation of protein-disulfide isomerase was perturbed, we propose that a deficiency in protein glycosylation is responsible for the observed cell death phenotype.
Keywords
L-ASCORBIC-ACID, CELLULOSE BIOSYNTHESIS, DISULFIDE-ISOMERASE, GLUCOSIDASE-I, HIGHER-PLANTS, GDP-MANNOSE, VITAMIN-C, DEFICIENT, MUTANTS, OLIGOSACCHARYLTRANSFERASE

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Chicago
Hoeberichts, Frank, Olivier Van Aken, Guy Kiddle, Emmy Coppens, Brigitte Van De Cotte, Antoine Adamantidis, Sandra Ormenese, et al. 2008. “A Temperature-sensitive Mutation in the Arabidopsis Thaliana Phosphomannomutase Gene Disrupts Protein Glycosylation and Triggers Cell Death.” Journal of Biological Chemistry 283 (9): 5708–5718.
APA
Hoeberichts, F., Van Aken, O., Kiddle, G., Coppens, E., Van De Cotte, B., Adamantidis, A., Ormenese, S., et al. (2008). A temperature-sensitive mutation in the Arabidopsis thaliana phosphomannomutase gene disrupts protein glycosylation and triggers cell death. JOURNAL OF BIOLOGICAL CHEMISTRY, 283(9), 5708–5718.
Vancouver
1.
Hoeberichts F, Van Aken O, Kiddle G, Coppens E, Van De Cotte B, Adamantidis A, et al. A temperature-sensitive mutation in the Arabidopsis thaliana phosphomannomutase gene disrupts protein glycosylation and triggers cell death. JOURNAL OF BIOLOGICAL CHEMISTRY. 2008;283(9):5708–18.
MLA
Hoeberichts, Frank, Olivier Van Aken, Guy Kiddle, et al. “A Temperature-sensitive Mutation in the Arabidopsis Thaliana Phosphomannomutase Gene Disrupts Protein Glycosylation and Triggers Cell Death.” JOURNAL OF BIOLOGICAL CHEMISTRY 283.9 (2008): 5708–5718. Print.
@article{408338,
  abstract     = {Eukaryotic phosphomannomutases (PMMs) catalyze the interconversion of mannose 6-phosphate to mannose 1-phosphate and are essential to the biosynthesis of GDP-mannose. As such, plant PMMs are involved in ascorbic acid (AsA) biosynthesis and N-glycosylation. We report on the conditional phenotype of the temperature-sensitive Arabidopsis thaliana pmm- 12 mutant. Mutant seedlings were phenotypically similar to wild type seedlings when grown at 16 - 18 degrees C but died within several days after transfer to 28 degrees C. This phenotype was observed throughout both vegetative and reproductive development. Protein extracts derived from pmm-12 plants had lower PMM protein and enzyme activity levels. In vitro biochemical analysis of recombinant proteins showed that the mutant PMM protein was compromised in its catalytic efficiency (K-cat/K-m). Despite significantly decreased AsA levels in pmm-12 plants, AsA deficiency could not account for the observed phenotype. Since, at restrictive temperature, total glycoprotein patterns were altered and glycosylation of protein-disulfide isomerase was perturbed, we propose that a deficiency in protein glycosylation is responsible for the observed cell death phenotype.},
  author       = {Hoeberichts, Frank and Van Aken, Olivier and Kiddle, Guy and Coppens, Emmy and Van De Cotte, Brigitte and Adamantidis, Antoine and Ormenese, Sandra and Foyer, Christine H and Zabeau, Marc and Inz{\'e}, Dirk and P{\'e}rilleux, Claire and Van Breusegem, Frank and Vuylsteke, Marnik},
  issn         = {0021-9258},
  journal      = {JOURNAL OF BIOLOGICAL CHEMISTRY},
  keyword      = {L-ASCORBIC-ACID,CELLULOSE BIOSYNTHESIS,DISULFIDE-ISOMERASE,GLUCOSIDASE-I,HIGHER-PLANTS,GDP-MANNOSE,VITAMIN-C,DEFICIENT,MUTANTS,OLIGOSACCHARYLTRANSFERASE},
  language     = {eng},
  number       = {9},
  pages        = {5708--5718},
  title        = {A temperature-sensitive mutation in the Arabidopsis thaliana phosphomannomutase gene disrupts protein glycosylation and triggers cell death},
  url          = {http://dx.doi.org/10.1074/jbc.M704991200},
  volume       = {283},
  year         = {2008},
}

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