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Extending the honey bee venome with the antimicrobial peptide apidaecin and a protein resembling wasp antigen 5

(2013) INSECT MOLECULAR BIOLOGY. 22(2). p.199-210
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Abstract
Honey bee venom is a complex mixture of toxic proteins and peptides. In the present study we tried to extend our knowledge of the venom composition using two different approaches. First, worker venom was analysed by liquid chromatography-mass spectrometry and this revealed the antimicrobial peptide apidaecin for the first time in such samples. Its expression in the venom gland was confirmed by reverse transcription PCR and by a peptidomic analysis of the venom apparatus tissue. Second, genome mining revealed a list of proteins with resemblance to known insect allergens or venom toxins, one of which showed homology to proteins of the antigen 5 (Ag5)/Sol i 3 cluster. It was demonstrated that the honey bee Ag5-like gene is expressed by venom gland tissue of winter bees but not of summer bees. Besides this seasonal variation, it shows an interesting spatial expression pattern with additional production in the hypopharyngeal glands, the brains and the midgut. Finally, our immunoblot study revealed that both synthetic apidaecin and the Ag5-like recombinant from bacteria evoke no humoral activity in beekeepers. Also, no IgG4-based cross-reactivity was detected between the honey bee Ag5-like protein and its yellow jacket paralogue Ves v 5.
Keywords
honey bee, venom, Apis mellifera, apidaecin, antigen 5, APIS-MELLIFERA, STRUCTURAL CHARACTERIZATION, EXPRESSION, MECHANISMS, ALLERGENS, DEFENSE, BIODIVERSITY, SUPERFAMILY, COMPONENT, GENOME

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Chicago
Van Vaerenbergh, Matthias, Dries Cardoen, Ellen Formesyn, Marleen Brunain, Gonzalez Van Driessche, S Blank, E Spillner, et al. 2013. “Extending the Honey Bee Venome with the Antimicrobial Peptide Apidaecin and a Protein Resembling Wasp Antigen 5.” Insect Molecular Biology 22 (2): 199–210.
APA
Van Vaerenbergh, M., Cardoen, D., Formesyn, E., Brunain, M., Van Driessche, G., Blank, S., Spillner, E., et al. (2013). Extending the honey bee venome with the antimicrobial peptide apidaecin and a protein resembling wasp antigen 5. INSECT MOLECULAR BIOLOGY, 22(2), 199–210.
Vancouver
1.
Van Vaerenbergh M, Cardoen D, Formesyn E, Brunain M, Van Driessche G, Blank S, et al. Extending the honey bee venome with the antimicrobial peptide apidaecin and a protein resembling wasp antigen 5. INSECT MOLECULAR BIOLOGY. 2013;22(2):199–210.
MLA
Van Vaerenbergh, Matthias, Dries Cardoen, Ellen Formesyn, et al. “Extending the Honey Bee Venome with the Antimicrobial Peptide Apidaecin and a Protein Resembling Wasp Antigen 5.” INSECT MOLECULAR BIOLOGY 22.2 (2013): 199–210. Print.
@article{4082923,
  abstract     = {Honey bee venom is a complex mixture of toxic proteins and peptides. In the present study we tried to extend our knowledge of the venom composition using two different approaches. First, worker venom was analysed by liquid chromatography-mass spectrometry and this revealed the antimicrobial peptide apidaecin for the first time in such samples. Its expression in the venom gland was confirmed by reverse transcription PCR and by a peptidomic analysis of the venom apparatus tissue. Second, genome mining revealed a list of proteins with resemblance to known insect allergens or venom toxins, one of which showed homology to proteins of the antigen 5 (Ag5)/Sol i 3 cluster. It was demonstrated that the honey bee Ag5-like gene is expressed by venom gland tissue of winter bees but not of summer bees. Besides this seasonal variation, it shows an interesting spatial expression pattern with additional production in the hypopharyngeal glands, the brains and the midgut. Finally, our immunoblot study revealed that both synthetic apidaecin and the Ag5-like recombinant from bacteria evoke no humoral activity in beekeepers. Also, no IgG4-based cross-reactivity was detected between the honey bee Ag5-like protein and its yellow jacket paralogue Ves v 5.},
  author       = {Van Vaerenbergh, Matthias and Cardoen, Dries and Formesyn, Ellen and Brunain, Marleen and Van Driessche, Gonzalez and Blank, S and Spillner, E and Verleyen, Peter and Wenseleers, Tom and Schoofs, Liliane and Devreese, Bart and de Graaf, Dirk},
  issn         = {0962-1075},
  journal      = {INSECT MOLECULAR BIOLOGY},
  language     = {eng},
  number       = {2},
  pages        = {199--210},
  title        = {Extending the honey bee venome with the antimicrobial peptide apidaecin and a protein resembling wasp antigen 5},
  url          = {http://dx.doi.org/10.1111/imb.12013},
  volume       = {22},
  year         = {2013},
}

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