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Determination of the wax layer ultrastructure of Jonagold with confocal and raster electron microscopy

EA Veraverbeke, B Nicolaï, Patric Van Oostveldt UGent and J De Baerdemaeker (2000) Acta Horticulturae. 517. p.389-395
abstract
Until now, techniques for measuring wax layer thickness and analysing wax layer ultrastructure were always destructive and could alter the original structure of the wax. In this research confocal microscopy and raster electron microscopy were evaluated and compared to conventional scanning electron microscopy. Both new techniques have the advantage that minimal sample preparation is needed compared to conventional SEM. From the images obtained by means of confocal microscopy, no semicrystalline, flaked structure as described in literature could be observed. In contrary, a porous structure with little irregular channels was observed. At the outer surface, cracks could be observed which corresponded to the boundary edges of the epidermal cells beneath the cuticula. This was confirmed by REM. On several confocal microscopy images small droplets were observed at the surface of the wax layer. The exact origine, formation and composition of these droplets was not yet revealed. They were very unstable for they disappeared more or less when more severe techniques as SEM or REM were applied. The thickness of the wax layer could also be determined from the images, and was found to be typically between 10 to 60 mu m.
Please use this url to cite or link to this publication:
author
organization
year
type
conference (proceedingsPaper)
publication status
published
subject
keyword
wax, Jonagold, raster electron microscopy, confocal microscopy
in
Acta Horticulturae
Acta Hortic.
editor
M Herregods
volume
517
issue title
Proceedings of the XXV international horticultural congress, part 7 : quality of horticultural products
pages
389 - 395
publisher
International Society for Horticultural Science (ISHS)
place of publication
Leuven, Belgium
conference name
25th International Horticultural Congress
conference location
Brussels, Belgium
conference start
1998-08-02
conference end
1998-08-07
Web of Science type
Article
Web of Science id
000088354600049
ISSN
0567-7572
ISBN
9789066058132
language
English
UGent publication?
yes
classification
P1
id
407215
handle
http://hdl.handle.net/1854/LU-407215
alternative location
http://www.actahort.org/books/517/517_49.htm
date created
2008-05-14 13:48:00
date last changed
2017-05-02 12:29:14
@inproceedings{407215,
  abstract     = {Until now, techniques for measuring wax layer thickness and analysing wax layer ultrastructure were always destructive and could alter the original structure of the wax. In this research confocal microscopy and raster electron microscopy were evaluated and compared to conventional scanning electron microscopy. Both new techniques have the advantage that minimal sample preparation is needed compared to conventional SEM. From the images obtained by means of confocal microscopy, no semicrystalline, flaked structure as described in literature could be observed. In contrary, a porous structure with little irregular channels was observed. At the outer surface, cracks could be observed which corresponded to the boundary edges of the epidermal cells beneath the cuticula. This was confirmed by REM. On several confocal microscopy images small droplets were observed at the surface of the wax layer. The exact origine, formation and composition of these droplets was not yet revealed. They were very unstable for they disappeared more or less when more severe techniques as SEM or REM were applied. The thickness of the wax layer could also be determined from the images, and was found to be typically between 10 to 60 mu m.},
  author       = {Veraverbeke, EA and Nicola{\"i}, B and Van Oostveldt, Patric and De Baerdemaeker, J},
  booktitle    = {Acta Horticulturae},
  editor       = {Herregods, M},
  isbn         = {9789066058132},
  issn         = {0567-7572},
  keyword      = {wax,Jonagold,raster electron microscopy,confocal microscopy},
  language     = {eng},
  location     = {Brussels, Belgium},
  pages        = {389--395},
  publisher    = {International Society for Horticultural Science (ISHS)},
  title        = {Determination of the wax layer ultrastructure of Jonagold with confocal and raster electron microscopy},
  url          = {http://www.actahort.org/books/517/517\_49.htm},
  volume       = {517},
  year         = {2000},
}

Chicago
Veraverbeke, EA, B Nicolaï, Patric Van Oostveldt, and J De Baerdemaeker. 2000. “Determination of the Wax Layer Ultrastructure of Jonagold with Confocal and Raster Electron Microscopy.” In Acta Horticulturae, ed. M Herregods, 517:389–395. Leuven, Belgium: International Society for Horticultural Science (ISHS).
APA
Veraverbeke, E., Nicolaï, B., Van Oostveldt, P., & De Baerdemaeker, J. (2000). Determination of the wax layer ultrastructure of Jonagold with confocal and raster electron microscopy. In M. Herregods (Ed.), Acta Horticulturae (Vol. 517, pp. 389–395). Presented at the 25th International Horticultural Congress, Leuven, Belgium: International Society for Horticultural Science (ISHS).
Vancouver
1.
Veraverbeke E, Nicolaï B, Van Oostveldt P, De Baerdemaeker J. Determination of the wax layer ultrastructure of Jonagold with confocal and raster electron microscopy. In: Herregods M, editor. Acta Horticulturae. Leuven, Belgium: International Society for Horticultural Science (ISHS); 2000. p. 389–95.
MLA
Veraverbeke, EA, B Nicolaï, Patric Van Oostveldt, et al. “Determination of the Wax Layer Ultrastructure of Jonagold with Confocal and Raster Electron Microscopy.” Acta Horticulturae. Ed. M Herregods. Vol. 517. Leuven, Belgium: International Society for Horticultural Science (ISHS), 2000. 389–395. Print.