Advanced search
Add to list

A novel slow release approach to bioaugment more effectively activated sludge systems

Author
Organization
Abstract
The survival and activity of microbial degradative inoculants in bioreactors is critical to obtain successful biodegradation of non- or slowly degradable pollutants. Achieving this in industrial wastewater reactors is technically challenging. We evaluated a strategy to obtain complete and stable bioaugmentation of activated sludge, used to treat a 3-chloroaniline (3-CA) contaminated wastewater in a lab-scale Semi-Continuous Activated Sludge system. A 3-CA metabolizing bacterium, Comamonas testosteroni strain 12, was mixed with molten agar and encapsulated in open ended silicon tubes. The tubes containing the immobilized bacteria represented about 1% of the volume of the mixed liquor. The bioaugmentation activity of a reactor containing the immobilized cells was compared with a reactor with suspended I2gfp cells. From day 25-30 after inoculation, the reactor with only suspended cells failed to degrade 3-CA completely, due to a decrease in specific metabolic activity. In the reactors with immobilized cells however, 3-CA continued to be removed. A mass balance indicated that ca. 10% of the degradation activity was due to the immobilized cells. Slow release of the growing embedded cells from the agar into the activated sludge medium, resulting in a higher number of active 3-CA degrading 12 cells, was responsible for ca. 90% of the degradation. Our results demonstrate that this simple immobilization procedure was effective to maintain a 3-CA degrading population within the activated sludge community.
Keywords
SURVIVAL, 3-CHLOROANILINE

Citation

Please use this url to cite or link to this publication:

MLA
Boon, Nico, Leen De Gelder, Hanne Lievens, et al. “A Novel Slow Release Approach to Bioaugment More Effectively Activated Sludge Systems.” EUROPEAN SYMPOSIUM ON ENVIRONMENTAL BIOTECHNOLOGY, ESEB 2004. Ed. Willy Verstraete. Leiden, The Netherlands: AA Balkema Publishers, 2004. 93–97. Print.
APA
Boon, N., De Gelder, L., Lievens, H., Verstraete, W., Siciliano, S., & Top, E. M. (2004). A novel slow release approach to bioaugment more effectively activated sludge systems. In Willy Verstraete (Ed.), EUROPEAN SYMPOSIUM ON ENVIRONMENTAL BIOTECHNOLOGY, ESEB 2004 (pp. 93–97). Presented at the European Symposium on Environmental Biotechnology, Leiden, The Netherlands: AA Balkema Publishers.
Chicago author-date
Boon, Nico, Leen De Gelder, Hanne Lievens, Willy Verstraete, Steven Siciliano, and Eva M Top. 2004. “A Novel Slow Release Approach to Bioaugment More Effectively Activated Sludge Systems.” In EUROPEAN SYMPOSIUM ON ENVIRONMENTAL BIOTECHNOLOGY, ESEB 2004, ed. Willy Verstraete, 93–97. Leiden, The Netherlands: AA Balkema Publishers.
Chicago author-date (all authors)
Boon, Nico, Leen De Gelder, Hanne Lievens, Willy Verstraete, Steven Siciliano, and Eva M Top. 2004. “A Novel Slow Release Approach to Bioaugment More Effectively Activated Sludge Systems.” In EUROPEAN SYMPOSIUM ON ENVIRONMENTAL BIOTECHNOLOGY, ESEB 2004, ed. Willy Verstraete, 93–97. Leiden, The Netherlands: AA Balkema Publishers.
Vancouver
1.
Boon N, De Gelder L, Lievens H, Verstraete W, Siciliano S, Top EM. A novel slow release approach to bioaugment more effectively activated sludge systems. In: Verstraete W, editor. EUROPEAN SYMPOSIUM ON ENVIRONMENTAL BIOTECHNOLOGY, ESEB 2004. Leiden, The Netherlands: AA Balkema Publishers; 2004. p. 93–7.
IEEE
[1]
N. Boon, L. De Gelder, H. Lievens, W. Verstraete, S. Siciliano, and E. M. Top, “A novel slow release approach to bioaugment more effectively activated sludge systems,” in EUROPEAN SYMPOSIUM ON ENVIRONMENTAL BIOTECHNOLOGY, ESEB 2004, Oostende, Belgium, 2004, pp. 93–97.
@inproceedings{403993,
  abstract     = {The survival and activity of microbial degradative inoculants in bioreactors is critical to obtain successful biodegradation of non- or slowly degradable pollutants. Achieving this in industrial wastewater reactors is technically challenging. We evaluated a strategy to obtain complete and stable bioaugmentation of activated sludge, used to treat a 3-chloroaniline (3-CA) contaminated wastewater in a lab-scale Semi-Continuous Activated Sludge system. A 3-CA metabolizing bacterium, Comamonas testosteroni strain 12, was mixed with molten agar and encapsulated in open ended silicon tubes. The tubes containing the immobilized bacteria represented about 1% of the volume of the mixed liquor. The bioaugmentation activity of a reactor containing the immobilized cells was compared with a reactor with suspended I2gfp cells. From day 25-30 after inoculation, the reactor with only suspended cells failed to degrade 3-CA completely, due to a decrease in specific metabolic activity. In the reactors with immobilized cells however, 3-CA continued to be removed. A mass balance indicated that ca. 10% of the degradation activity was due to the immobilized cells. Slow release of the growing embedded cells from the agar into the activated sludge medium, resulting in a higher number of active 3-CA degrading 12 cells, was responsible for ca. 90% of the degradation. Our results demonstrate that this simple immobilization procedure was effective to maintain a 3-CA degrading population within the activated sludge community.},
  author       = {Boon, Nico and De Gelder, Leen and Lievens, Hanne and Verstraete, Willy and Siciliano, Steven and Top, Eva M},
  booktitle    = {EUROPEAN SYMPOSIUM ON ENVIRONMENTAL BIOTECHNOLOGY, ESEB 2004},
  editor       = {Verstraete, Willy},
  isbn         = {90-5809-653-X},
  keywords     = {SURVIVAL,3-CHLOROANILINE},
  language     = {eng},
  location     = {Oostende, Belgium},
  pages        = {93--97},
  publisher    = {AA Balkema Publishers},
  title        = {A novel slow release approach to bioaugment more effectively activated sludge systems},
  year         = {2004},
}

Web of Science
Times cited: