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Introduction of nematode-inducible plant promoter-gus fusions in hairy roots of different plants

Mansour Karimi (UGent) , Kris Van Poucke (UGent) , Nathalie Barthels (UGent) , Marc Van Montagu (UGent) and Godelieve Gheysen (UGent)
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Abstract
Plant-parasitic nematodes are among the most important pests of agricultural plants worldwide. They infect plants and establish complex feeding sites inside the root tissues. Plant-parasitic nematodes are controlled to same extent using conventional methods, These methods are either not efficient or cause environmental hazard. An alternative and promising way is making nematode-resistant plants by introducing engineered genes. Using die promoter tagging approach, we have identified some nematode-inducible plant promoters. After isolation of these promoters, they have been fused to the beta-glucuronidase (gus) marker. We have introduced the promoter-gus fusions into different plant species via Agrobacterium tumefaciens or Agrobacterium rhizogenes. Since plant-parasitic nematodes invade the plant root, we found re-introduction of plant promoters by using A. rhizogenes relatively faster and easier. To get a hgh frequency of transformation and hairy root regeneration, plant media and hormone compositions mere optimised. Then a number of plant species were transformed using A. rhizogenes pRil5834. The Agrobacterium harboured a binary vector containing a T-DNA composed of a nematode-inducible promoter gus-fusion and nptII gene. Integration of the T-DNAs from the binary vector and the Ri plasmid results in the generation of kanamycin-resistant hairy roots.

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MLA
Karimi, Mansour, Kris Van Poucke, Nathalie Barthels, et al. “Introduction of Nematode-inducible Plant Promoter-gus Fusions in Hairy Roots of Different Plants.” Mededelingen Van De Faculteit Landbouwkundige En Toegepaste Biologische Wetenschappen, Universiteit Gent. Vol. 63. 1998. 609–617. Print.
APA
Karimi, M., Van Poucke, K., Barthels, N., Van Montagu, M., & Gheysen, G. (1998). Introduction of nematode-inducible plant promoter-gus fusions in hairy roots of different plants. MEDEDELINGEN VAN DE FACULTEIT LANDBOUWKUNDIGE EN TOEGEPASTE BIOLOGISCHE WETENSCHAPPEN, UNIVERSITEIT GENT (Vol. 63, pp. 609–617). Presented at the 50th International symposium on Crop Protection.
Chicago author-date
Karimi, Mansour, Kris Van Poucke, Nathalie Barthels, Marc Van Montagu, and Godelieve Gheysen. 1998. “Introduction of Nematode-inducible Plant Promoter-gus Fusions in Hairy Roots of Different Plants.” In Mededelingen Van De Faculteit Landbouwkundige En Toegepaste Biologische Wetenschappen, Universiteit Gent, 63:609–617.
Chicago author-date (all authors)
Karimi, Mansour, Kris Van Poucke, Nathalie Barthels, Marc Van Montagu, and Godelieve Gheysen. 1998. “Introduction of Nematode-inducible Plant Promoter-gus Fusions in Hairy Roots of Different Plants.” In Mededelingen Van De Faculteit Landbouwkundige En Toegepaste Biologische Wetenschappen, Universiteit Gent, 63:609–617.
Vancouver
1.
Karimi M, Van Poucke K, Barthels N, Van Montagu M, Gheysen G. Introduction of nematode-inducible plant promoter-gus fusions in hairy roots of different plants. MEDEDELINGEN VAN DE FACULTEIT LANDBOUWKUNDIGE EN TOEGEPASTE BIOLOGISCHE WETENSCHAPPEN, UNIVERSITEIT GENT. 1998. p. 609–17.
IEEE
[1]
M. Karimi, K. Van Poucke, N. Barthels, M. Van Montagu, and G. Gheysen, “Introduction of nematode-inducible plant promoter-gus fusions in hairy roots of different plants,” in MEDEDELINGEN VAN DE FACULTEIT LANDBOUWKUNDIGE EN TOEGEPASTE BIOLOGISCHE WETENSCHAPPEN, UNIVERSITEIT GENT, Ghent, Belgium, 1998, vol. 63, no. 3a, pp. 609–617.
@inproceedings{399761,
  abstract     = {Plant-parasitic nematodes are among the most important pests of agricultural plants worldwide. They infect plants and establish complex feeding sites inside the root tissues. Plant-parasitic nematodes are controlled to same extent using conventional methods, These methods are either not efficient or cause environmental hazard. An alternative and promising way is making nematode-resistant plants by introducing engineered genes. Using die promoter tagging approach, we have identified some nematode-inducible plant promoters. After isolation of these promoters, they have been fused to the beta-glucuronidase (gus) marker. We have introduced the promoter-gus fusions into different plant species via Agrobacterium tumefaciens or Agrobacterium rhizogenes. Since plant-parasitic nematodes invade the plant root, we found re-introduction of plant promoters by using A. rhizogenes relatively faster and easier. To get a hgh frequency of transformation and hairy root regeneration, plant media and hormone compositions mere optimised. Then a number of plant species were transformed using A. rhizogenes pRil5834. The Agrobacterium harboured a binary vector containing a T-DNA composed of a nematode-inducible promoter gus-fusion and nptII gene. Integration of the T-DNAs from the binary vector and the Ri plasmid results in the generation of kanamycin-resistant hairy roots.},
  author       = {Karimi, Mansour and Van Poucke, Kris and Barthels, Nathalie and Van Montagu, Marc and Gheysen, Godelieve},
  booktitle    = {MEDEDELINGEN VAN DE FACULTEIT LANDBOUWKUNDIGE EN TOEGEPASTE BIOLOGISCHE WETENSCHAPPEN, UNIVERSITEIT GENT},
  issn         = {1373-7503},
  language     = {eng},
  location     = {Ghent, Belgium},
  number       = {3a},
  pages        = {609--617},
  title        = {Introduction of nematode-inducible plant promoter-gus fusions in hairy roots of different plants},
  volume       = {63},
  year         = {1998},
}

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