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Higher extractability of lignin in poplar (Populus tremula x P. alba) by reducing cinnamyl alcohol dehydrogenase activity

(1996) Forestry Sciences. 49. p.153-158
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Abstract
Cinnamyl alcohol dehydrogenase (CAD) catalyses the last step in the biosynthesis of the lignin precursors, which is the conversion of cinnamaldehydes into cinnamyl alcohols. A poplar (P. trichocarpa x P. deltoides) CAD cDNA was introduced in sense and in antisense orientation into P. tremula x P. alba via an Agrobacterium-mediated transformation. The CAD activity was reduced by 70% in the xylem tissue of three antisense and two co-suppressed lines. A red coloration of the xylem was detected in all poplar lines with a residual CAD activity of less than 60%. The lignin content and the lignin composition (syringyl/guaiacyl) were similar in co-suppressed, antisense CAD and control lines. However, chemical properties of the cell wall were different. First, a phloroglucinol staining showed a different coloration in the xylem of the down-regulated CAD poplars, being red-brown as compared to the typical pink staining of the control xylem. Second, after NaOH treatment, lignin could be precipitated by acidification of the alkali-soluble fraction of the cell wall of the red poplars but not of that of the white poplars. A spectral characterisation of the alkali fraction from red poplars showed an increased level of vanillin and syringaldehyde. Third, pulping experiments showed less residual lignin in the pulp of CAD down-regulated poplars.

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MLA
Baucher, Marie, Brigitte Chabbert, Jan Van Doorsselaere, et al. “Higher Extractability of Lignin in Poplar (Populus Tremula x P. Alba) by Reducing Cinnamyl Alcohol Dehydrogenase Activity.” Forestry Sciences. Ed. MR Ahuja, Wout Boerjan, & DB Neale. Vol. 49. Dordrecht, The Netherlands: Kluwer Academic, 1996. 153–158. Print.
APA
Baucher, M., Chabbert, B., Van Doorsselaere, J., Pilate, G., Cornu, D., Petit-Conil, M., Monties, B., et al. (1996). Higher extractability of lignin in poplar (Populus tremula x P. alba) by reducing cinnamyl alcohol dehydrogenase activity. In M. Ahuja, W. Boerjan, & D. Neale (Eds.), Forestry Sciences (Vol. 49, pp. 153–158). Presented at the Joint meeting of IUFRO Working Party on Somatic Cell Genetics / IUFRO Working Party on Molecular Genetics on Somatic Cell Genetics and Molecular Genetics of Trees, Dordrecht, The Netherlands: Kluwer Academic.
Chicago author-date
Baucher, Marie, Brigitte Chabbert, Jan Van Doorsselaere, Gilles Pilate, Daniel Cornu, Michel Petit-Conil, Bernard Monties, et al. 1996. “Higher Extractability of Lignin in Poplar (Populus Tremula x P. Alba) by Reducing Cinnamyl Alcohol Dehydrogenase Activity.” In Forestry Sciences, ed. MR Ahuja, Wout Boerjan, and DB Neale, 49:153–158. Dordrecht, The Netherlands: Kluwer Academic.
Chicago author-date (all authors)
Baucher, Marie, Brigitte Chabbert, Jan Van Doorsselaere, Gilles Pilate, Daniel Cornu, Michel Petit-Conil, Bernard Monties, Marc Van Montagu, Dirk Inzé, Lise Jouanin, and Wout Boerjan. 1996. “Higher Extractability of Lignin in Poplar (Populus Tremula x P. Alba) by Reducing Cinnamyl Alcohol Dehydrogenase Activity.” In Forestry Sciences, ed. MR Ahuja, Wout Boerjan, and DB Neale, 49:153–158. Dordrecht, The Netherlands: Kluwer Academic.
Vancouver
1.
Baucher M, Chabbert B, Van Doorsselaere J, Pilate G, Cornu D, Petit-Conil M, et al. Higher extractability of lignin in poplar (Populus tremula x P. alba) by reducing cinnamyl alcohol dehydrogenase activity. In: Ahuja M, Boerjan W, Neale D, editors. Forestry Sciences. Dordrecht, The Netherlands: Kluwer Academic; 1996. p. 153–8.
IEEE
[1]
M. Baucher et al., “Higher extractability of lignin in poplar (Populus tremula x P. alba) by reducing cinnamyl alcohol dehydrogenase activity,” in Forestry Sciences, Ghent, Belgium, 1996, vol. 49, pp. 153–158.
@inproceedings{398448,
  abstract     = {Cinnamyl alcohol dehydrogenase (CAD) catalyses the last step in the biosynthesis of the lignin precursors, which is the conversion of cinnamaldehydes into cinnamyl alcohols. A poplar (P. trichocarpa x P. deltoides) CAD cDNA was introduced in sense and in antisense orientation into P. tremula x P. alba via an Agrobacterium-mediated transformation. The CAD activity was reduced by 70% in the xylem tissue of three antisense and two co-suppressed lines. A red coloration of the xylem was detected in all poplar lines with a residual CAD activity of less than 60%. The lignin content and the lignin composition (syringyl/guaiacyl) were similar in co-suppressed, antisense CAD and control lines. However, chemical properties of the cell wall were different. First, a phloroglucinol staining showed a different coloration in the xylem of the down-regulated CAD poplars, being red-brown as compared to the typical pink staining of the control xylem. Second, after NaOH treatment, lignin could be precipitated by acidification of the alkali-soluble fraction of the cell wall of the red poplars but not of that of the white poplars. A spectral characterisation of the alkali fraction from red poplars showed an increased level of vanillin and syringaldehyde. Third, pulping experiments showed less residual lignin in the pulp of CAD down-regulated poplars.},
  author       = {Baucher, Marie and Chabbert, Brigitte and Van Doorsselaere, Jan and Pilate, Gilles and Cornu, Daniel and Petit-Conil, Michel and Monties, Bernard and Van Montagu, Marc and Inzé, Dirk and Jouanin, Lise and Boerjan, Wout},
  booktitle    = {Forestry Sciences},
  editor       = {Ahuja, MR and Boerjan, Wout and Neale, DB},
  isbn         = {9780792341796},
  language     = {eng},
  location     = {Ghent, Belgium},
  pages        = {153--158},
  publisher    = {Kluwer Academic},
  title        = {Higher extractability of lignin in poplar (Populus tremula x P. alba) by reducing cinnamyl alcohol dehydrogenase activity},
  volume       = {49},
  year         = {1996},
}