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Development of a method for assessing the relative contribution of waterborne and dietary exposure to zinc bioaccumulation in Daphnia magna by using isotopically enriched tracers and ICP-MS detection

Lieve Balcaen UGent, Karel De Schamphelaere UGent, Colin Janssen UGent, Luc Moens UGent and Frank Vanhaecke UGent (2008) ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 390(2). p.555-569
abstract
In order to study the effect of anthropogenic substances on freshwater and marine ecosystems and to develop methods to derive water-quality criteria, ecotoxicological testing is required. While toxicity assessments are traditionally based on dissolved metal concentrations, assuming that toxicity is caused by waterborne metal only, it was recently pointed out that also the dietary exposure route should be carefully considered and interpreted in regulatory assessments of zinc. In this context, the aim of this experimental study was to develop a method which allows the uptake of waterborne and dietary zinc by Daphnia magna and the interaction between both exposure routes to be studied. Therefore, the setup of a dual isotopic tracer study was required. During several days, daphnids were exposed to Zn-67 and Zn-68 via the dietary and the waterborne routes, respectively, and after several time intervals the daphnids were sampled and subjected to isotopic analysis by means of inductively coupled plasma mass spectrometry (ICP-MS). In order to obtain reliable and accurate results for zinc, special care was taken to prevent contamination and to deal with the spectral interferences traditionally hindering the determination of zinc. The figures of merit of both a quadrupole-based ICP-MS instrument equipped with a dynamic reaction cell, and a sector field ICP-MS unit were studied, and it was concluded that by using a sector field mass spectrometer operated at medium mass resolution all interferences could be overcome adequately. Although the set-up of the exposure experiments seems to be rather simple at first sight, it was shown in this work that several (dynamic) variables can have an influence on the results obtained and on the subsequent data interpretation. The importance of these confounding factors was examined, and on the basis of preliminary calculations it became clear that not only the isotopic composition of the daphnids has to be studied-adequate monitoring of the isotopic composition of the dissolved phase and the algae during the exposure of the daphnids is also required to accurately discriminate between uptake from water and from food.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
URINE, CD, ELEMENTS, ASSIMILATION, ACCUMULATION, TOXICITY, ZN, COPPER, TRANSITION-METALS, PLASMA-MASS SPECTROMETRY, ecotoxicology, interferences, ICP-mass spectrometry, tracer study, isotopic analysis
journal title
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Anal. Bioanal. Chem.
volume
390
issue
2
pages
555 - 569
Web of Science type
Article
Web of Science id
000252170400014
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
3.328 (2008)
JCR rank
9/70 (2008)
JCR quartile
1 (2008)
ISSN
1618-2642
DOI
10.1007/s00216-007-1620-5
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
396342
handle
http://hdl.handle.net/1854/LU-396342
date created
2008-04-07 21:40:00
date last changed
2011-08-12 09:44:06
@article{396342,
  abstract     = {In order to study the effect of anthropogenic substances on freshwater and marine ecosystems and to develop methods to derive water-quality criteria, ecotoxicological testing is required. While toxicity assessments are traditionally based on dissolved metal concentrations, assuming that toxicity is caused by waterborne metal only, it was recently pointed out that also the dietary exposure route should be carefully considered and interpreted in regulatory assessments of zinc. In this context, the aim of this experimental study was to develop a method which allows the uptake of waterborne and dietary zinc by Daphnia magna and the interaction between both exposure routes to be studied. Therefore, the setup of a dual isotopic tracer study was required. During several days, daphnids were exposed to Zn-67 and Zn-68 via the dietary and the waterborne routes, respectively, and after several time intervals the daphnids were sampled and subjected to isotopic analysis by means of inductively coupled plasma mass spectrometry (ICP-MS). In order to obtain reliable and accurate results for zinc, special care was taken to prevent contamination and to deal with the spectral interferences traditionally hindering the determination of zinc. The figures of merit of both a quadrupole-based ICP-MS instrument equipped with a dynamic reaction cell, and a sector field ICP-MS unit were studied, and it was concluded that by using a sector field mass spectrometer operated at medium mass resolution all interferences could be overcome adequately. Although the set-up of the exposure experiments seems to be rather simple at first sight, it was shown in this work that several (dynamic) variables can have an influence on the results obtained and on the subsequent data interpretation. The importance of these confounding factors was examined, and on the basis of preliminary calculations it became clear that not only the isotopic composition of the daphnids has to be studied-adequate monitoring of the isotopic composition of the dissolved phase and the algae during the exposure of the daphnids is also required to accurately discriminate between uptake from water and from food.},
  author       = {Balcaen, Lieve and De Schamphelaere, Karel and Janssen, Colin and Moens, Luc and Vanhaecke, Frank},
  issn         = {1618-2642},
  journal      = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
  keyword      = {URINE,CD,ELEMENTS,ASSIMILATION,ACCUMULATION,TOXICITY,ZN,COPPER,TRANSITION-METALS,PLASMA-MASS SPECTROMETRY,ecotoxicology,interferences,ICP-mass spectrometry,tracer study,isotopic analysis},
  language     = {eng},
  number       = {2},
  pages        = {555--569},
  title        = {Development of a method for assessing the relative contribution of waterborne and dietary exposure to zinc bioaccumulation in Daphnia magna by using isotopically enriched tracers and ICP-MS detection},
  url          = {http://dx.doi.org/10.1007/s00216-007-1620-5},
  volume       = {390},
  year         = {2008},
}

Chicago
Balcaen, Lieve, Karel De Schamphelaere, Colin Janssen, Luc Moens, and Frank Vanhaecke. 2008. “Development of a Method for Assessing the Relative Contribution of Waterborne and Dietary Exposure to Zinc Bioaccumulation in Daphnia Magna by Using Isotopically Enriched Tracers and ICP-MS Detection.” Analytical and Bioanalytical Chemistry 390 (2): 555–569.
APA
Balcaen, L., De Schamphelaere, K., Janssen, C., Moens, L., & Vanhaecke, F. (2008). Development of a method for assessing the relative contribution of waterborne and dietary exposure to zinc bioaccumulation in Daphnia magna by using isotopically enriched tracers and ICP-MS detection. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 390(2), 555–569.
Vancouver
1.
Balcaen L, De Schamphelaere K, Janssen C, Moens L, Vanhaecke F. Development of a method for assessing the relative contribution of waterborne and dietary exposure to zinc bioaccumulation in Daphnia magna by using isotopically enriched tracers and ICP-MS detection. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2008;390(2):555–69.
MLA
Balcaen, Lieve, Karel De Schamphelaere, Colin Janssen, et al. “Development of a Method for Assessing the Relative Contribution of Waterborne and Dietary Exposure to Zinc Bioaccumulation in Daphnia Magna by Using Isotopically Enriched Tracers and ICP-MS Detection.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY 390.2 (2008): 555–569. Print.