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Metabolites produced by Pseudomonas sp. enable a Gram-positive bacterium to achieve extracellular electron transfer

Hai The Pham UGent, Nico Boon UGent, Peter Aelterman UGent, Peter Clauwaert UGent, Liesje De Schamphelaire UGent, Lynn Vanhaecke UGent, Katrien De Maeyer UGent, Monica Höfte UGent, Willy Verstraete UGent and Korneel Rabaey UGent (2008) APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 77(5). p.1119-1129
abstract
Previous studies revealed the abundance of Pseudomonas sp. in the microbial community of a microbial fuel cell (MFC). These bacteria can transfer electrons to the electrode via self-produced phenazine-based mediators. A MFC fed with acetate where several Pseudomonas sp. were present was found to be rich in a Gram-positive bacterium, identified as Brevibacillus sp. PTH1. Remarkably, MFCs operated with only the Brevibacillus strain in their anodes had poor electricity generation. Upon replacement of the anodic aqueous part of Brevibacillus containing MFCs with the cell-free anodic supernatants of MFCs operated with Pseudomonas sp. CMR12a, a strain producing considerable amounts of phenazine-1-carboxamide (PCN) and biosurfactants, the electricity generation was improved significantly. Supernatants of Pseudomonas sp. CMR12a_Reg, a regulatory mutant lacking the ability to produce PCN, had no similar improvement effect. Purified PCN, together with rhamnolipids as biosurfactants (1 mg L-1), could clearly improve electricity generation by Brevibacillus sp. PTH1, as well as enable this bacterium to oxidize acetate with concomitant reduction of ferric iron, supplied as goethite (FeOOH). When added alone, PCN had no observable effects on Brevibacillus' electron transfer. This work demonstrates that metabolites produced by Pseudomonas sp. enable Gram-positive bacteria to achieve extracellular electron transfer. Possibly, this bacterial interaction is a key process in the anodic electron transfer of a MFC, enabling Brevibacillus sp. PTH1 to achieve its dominance.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
MICROORGANISMS, electrochemically active bacteria, STRAIN, AERUGINOSA, BIOFUEL CELLS, ELECTRICITY PRODUCTION, FE(III)-REDUCING BACTERIUM, microbial interaction, electron shuttle, phenazines, biosurfactant, synergistic effect, MICROBIAL FUEL-CELLS, GRADIENT GEL-ELECTROPHORESIS, 16S RIBOSOMAL-RNA, GENERATION
journal title
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Appl. Microbiol. Biotechnol.
volume
77
issue
5
pages
1119 - 1129
Web of Science type
Article
Web of Science id
000251606900016
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
2.569 (2008)
JCR rank
50/144 (2008)
JCR quartile
2 (2008)
ISSN
0175-7598
DOI
10.1007/s00253-007-1248-6
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
391629
handle
http://hdl.handle.net/1854/LU-391629
date created
2008-03-06 14:34:00
date last changed
2014-01-06 14:22:48
@article{391629,
  abstract     = {Previous studies revealed the abundance of Pseudomonas sp. in the microbial community of a microbial fuel cell (MFC). These bacteria can transfer electrons to the electrode via self-produced phenazine-based mediators. A MFC fed with acetate where several Pseudomonas sp. were present was found to be rich in a Gram-positive bacterium, identified as Brevibacillus sp. PTH1. Remarkably, MFCs operated with only the Brevibacillus strain in their anodes had poor electricity generation. Upon replacement of the anodic aqueous part of Brevibacillus containing MFCs with the cell-free anodic supernatants of MFCs operated with Pseudomonas sp. CMR12a, a strain producing considerable amounts of phenazine-1-carboxamide (PCN) and biosurfactants, the electricity generation was improved significantly. Supernatants of Pseudomonas sp. CMR12a\_Reg, a regulatory mutant lacking the ability to produce PCN, had no similar improvement effect. Purified PCN, together with rhamnolipids as biosurfactants (1 mg L-1), could clearly improve electricity generation by Brevibacillus sp. PTH1, as well as enable this bacterium to oxidize acetate with concomitant reduction of ferric iron, supplied as goethite (FeOOH). When added alone, PCN had no observable effects on Brevibacillus' electron transfer. This work demonstrates that metabolites produced by Pseudomonas sp. enable Gram-positive bacteria to achieve extracellular electron transfer. Possibly, this bacterial interaction is a key process in the anodic electron transfer of a MFC, enabling Brevibacillus sp. PTH1 to achieve its dominance.},
  author       = {Pham, Hai The and Boon, Nico and Aelterman, Peter and Clauwaert, Peter and De Schamphelaire, Liesje and Vanhaecke, Lynn and De Maeyer, Katrien and H{\"o}fte, Monica and Verstraete, Willy and Rabaey, Korneel},
  issn         = {0175-7598},
  journal      = {APPLIED MICROBIOLOGY AND BIOTECHNOLOGY},
  keyword      = {MICROORGANISMS,electrochemically active bacteria,STRAIN,AERUGINOSA,BIOFUEL CELLS,ELECTRICITY PRODUCTION,FE(III)-REDUCING BACTERIUM,microbial interaction,electron shuttle,phenazines,biosurfactant,synergistic effect,MICROBIAL FUEL-CELLS,GRADIENT GEL-ELECTROPHORESIS,16S RIBOSOMAL-RNA,GENERATION},
  language     = {eng},
  number       = {5},
  pages        = {1119--1129},
  title        = {Metabolites produced by Pseudomonas sp. enable a Gram-positive bacterium to achieve extracellular electron transfer},
  url          = {http://dx.doi.org/10.1007/s00253-007-1248-6},
  volume       = {77},
  year         = {2008},
}

Chicago
Pham, Hai The, Nico Boon, Peter Aelterman, Peter Clauwaert, Liesje De Schamphelaire, Lynn Vanhaecke, Katrien De Maeyer, Monica Höfte, Willy Verstraete, and Korneel Rabaey. 2008. “Metabolites Produced by Pseudomonas Sp. Enable a Gram-positive Bacterium to Achieve Extracellular Electron Transfer.” Applied Microbiology and Biotechnology 77 (5): 1119–1129.
APA
Pham, H. T., Boon, N., Aelterman, P., Clauwaert, P., De Schamphelaire, L., Vanhaecke, L., De Maeyer, K., et al. (2008). Metabolites produced by Pseudomonas sp. enable a Gram-positive bacterium to achieve extracellular electron transfer. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 77(5), 1119–1129.
Vancouver
1.
Pham HT, Boon N, Aelterman P, Clauwaert P, De Schamphelaire L, Vanhaecke L, et al. Metabolites produced by Pseudomonas sp. enable a Gram-positive bacterium to achieve extracellular electron transfer. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2008;77(5):1119–29.
MLA
Pham, Hai The, Nico Boon, Peter Aelterman, et al. “Metabolites Produced by Pseudomonas Sp. Enable a Gram-positive Bacterium to Achieve Extracellular Electron Transfer.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 77.5 (2008): 1119–1129. Print.