Advanced search
1 file | 318.41 KB

Phenazines and biosurfactants interact in the biological control of soil-borne diseases caused by Pythium spp.

(2008) ENVIRONMENTAL MICROBIOLOGY. 10(3). p.778-788
Author
Organization
Abstract
In this study, the putative role of phenazines and rhamnolipid-biosurfactants, antagonistic metabolites produced by Pseudomonas aeruginosa PNA1, was tested in the biological control of Pythium splendens on bean (Phaseolus vulgaris L) and Pythium myriotylum on cocoyam (Xanthosoma sagittifolium L Schott). A rhamnolipid-deficient and a phenazine-deficient mutant of PNA1 were used either separately or jointly in plant experiments. When the mutants were applied separately, no disease-suppressive effect was observed, although both mutants still produced one of the antagonistic compounds (phenazines or rhamnolipids). When the mutants were concurrently introduced in the soil, the biocontrol activity was restored to wild-type levels. Bean seeds developed significantly less pre-emergence damping-off caused by P. splendens when treated with a mixture of purified phenazine-1-carboxamide and rhamnolipids than with any of the chemicals alone. When phenazines and rhamnolipids were combined at concentrations that had no observable effects when the metabolites were applied separately, mycelial growth of P. myriotylum was significantly reduced. In addition, microscopic analysis revealed substantial vacuolization and disintegration of Pythium hyphae after incubation in liquid medium amended with both metabolites. Results of this study indicate that phenazines and biosurfactants are acting synergistically in the control of Pythium spp.
Keywords
PHENAZINE-1-CARBOXYLIC ACID, ANTIFUNGAL ACTIVITY, FLUORESCENT PSEUDOMONAS, IN-VITRO, PHYTOPHTHORA-CRYPTOGEA, CHLORORAPHIS PCL1391, PSEUDOMONAS-AERUGINOSA PNA1, SUGAR-BEET RHIZOSPHERE, QUINOLONE SIGNAL, WITLOOF CHICORY

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 318.41 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Perneel, Maaike, Liesbet D’hondt, Katrien De Maeyer, Amayana Adiobo, Korneel Rabaey, and Monica Höfte. 2008. “Phenazines and Biosurfactants Interact in the Biological Control of Soil-borne Diseases Caused by Pythium Spp.” Environmental Microbiology 10 (3): 778–788.
APA
Perneel, M., D’hondt, L., De Maeyer, K., Adiobo, A., Rabaey, K., & Höfte, M. (2008). Phenazines and biosurfactants interact in the biological control of soil-borne diseases caused by Pythium spp. ENVIRONMENTAL MICROBIOLOGY, 10(3), 778–788.
Vancouver
1.
Perneel M, D’hondt L, De Maeyer K, Adiobo A, Rabaey K, Höfte M. Phenazines and biosurfactants interact in the biological control of soil-borne diseases caused by Pythium spp. ENVIRONMENTAL MICROBIOLOGY. 2008;10(3):778–88.
MLA
Perneel, Maaike, Liesbet D’hondt, Katrien De Maeyer, et al. “Phenazines and Biosurfactants Interact in the Biological Control of Soil-borne Diseases Caused by Pythium Spp.” ENVIRONMENTAL MICROBIOLOGY 10.3 (2008): 778–788. Print.
@article{391560,
  abstract     = {In this study, the putative role of phenazines and rhamnolipid-biosurfactants, antagonistic metabolites produced by Pseudomonas aeruginosa PNA1, was tested in the biological control of Pythium splendens on bean (Phaseolus vulgaris L) and Pythium myriotylum on cocoyam (Xanthosoma sagittifolium L Schott). A rhamnolipid-deficient and a phenazine-deficient mutant of PNA1 were used either separately or jointly in plant experiments. When the mutants were applied separately, no disease-suppressive effect was observed, although both mutants still produced one of the antagonistic compounds (phenazines or rhamnolipids). When the mutants were concurrently introduced in the soil, the biocontrol activity was restored to wild-type levels. Bean seeds developed significantly less pre-emergence damping-off caused by P. splendens when treated with a mixture of purified phenazine-1-carboxamide and rhamnolipids than with any of the chemicals alone. When phenazines and rhamnolipids were combined at concentrations that had no observable effects when the metabolites were applied separately, mycelial growth of P. myriotylum was significantly reduced. In addition, microscopic analysis revealed substantial vacuolization and disintegration of Pythium hyphae after incubation in liquid medium amended with both metabolites. Results of this study indicate that phenazines and biosurfactants are acting synergistically in the control of Pythium spp.},
  author       = {Perneel, Maaike and D'hondt, Liesbet and De Maeyer, Katrien and Adiobo, Amayana and Rabaey, Korneel and H{\"o}fte, Monica},
  issn         = {1462-2912},
  journal      = {ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {PHENAZINE-1-CARBOXYLIC ACID,ANTIFUNGAL ACTIVITY,FLUORESCENT PSEUDOMONAS,IN-VITRO,PHYTOPHTHORA-CRYPTOGEA,CHLORORAPHIS PCL1391,PSEUDOMONAS-AERUGINOSA PNA1,SUGAR-BEET RHIZOSPHERE,QUINOLONE SIGNAL,WITLOOF CHICORY},
  language     = {eng},
  number       = {3},
  pages        = {778--788},
  title        = {Phenazines and biosurfactants interact in the biological control of soil-borne diseases caused by Pythium spp.},
  url          = {http://dx.doi.org/10.1111/j.1462-2920.2007.01501.x},
  volume       = {10},
  year         = {2008},
}

Altmetric
View in Altmetric
Web of Science
Times cited: