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Phenazines and biosurfactants interact in the biological control of soil-borne diseases caused by Pythium spp.

Maaike Perneel, Liesbet D'hondt UGent, Katrien De Maeyer UGent, Amayana Adiobo, Korneel Rabaey UGent and Monica Höfte UGent (2008) ENVIRONMENTAL MICROBIOLOGY. 10(3). p.778-788
abstract
In this study, the putative role of phenazines and rhamnolipid-biosurfactants, antagonistic metabolites produced by Pseudomonas aeruginosa PNA1, was tested in the biological control of Pythium splendens on bean (Phaseolus vulgaris L) and Pythium myriotylum on cocoyam (Xanthosoma sagittifolium L Schott). A rhamnolipid-deficient and a phenazine-deficient mutant of PNA1 were used either separately or jointly in plant experiments. When the mutants were applied separately, no disease-suppressive effect was observed, although both mutants still produced one of the antagonistic compounds (phenazines or rhamnolipids). When the mutants were concurrently introduced in the soil, the biocontrol activity was restored to wild-type levels. Bean seeds developed significantly less pre-emergence damping-off caused by P. splendens when treated with a mixture of purified phenazine-1-carboxamide and rhamnolipids than with any of the chemicals alone. When phenazines and rhamnolipids were combined at concentrations that had no observable effects when the metabolites were applied separately, mycelial growth of P. myriotylum was significantly reduced. In addition, microscopic analysis revealed substantial vacuolization and disintegration of Pythium hyphae after incubation in liquid medium amended with both metabolites. Results of this study indicate that phenazines and biosurfactants are acting synergistically in the control of Pythium spp.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PHENAZINE-1-CARBOXYLIC ACID, ANTIFUNGAL ACTIVITY, FLUORESCENT PSEUDOMONAS, IN-VITRO, PHYTOPHTHORA-CRYPTOGEA, CHLORORAPHIS PCL1391, PSEUDOMONAS-AERUGINOSA PNA1, SUGAR-BEET RHIZOSPHERE, QUINOLONE SIGNAL, WITLOOF CHICORY
journal title
ENVIRONMENTAL MICROBIOLOGY
Environ. Microbiol.
volume
10
issue
3
pages
778 - 788
Web of Science type
Article
Web of Science id
000252712300021
JCR category
MICROBIOLOGY
JCR impact factor
4.707 (2008)
JCR rank
17/91 (2008)
JCR quartile
1 (2008)
ISSN
1462-2912
DOI
10.1111/j.1462-2920.2007.01501.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
391560
handle
http://hdl.handle.net/1854/LU-391560
date created
2008-03-06 10:38:00
date last changed
2012-02-03 10:08:29
@article{391560,
  abstract     = {In this study, the putative role of phenazines and rhamnolipid-biosurfactants, antagonistic metabolites produced by Pseudomonas aeruginosa PNA1, was tested in the biological control of Pythium splendens on bean (Phaseolus vulgaris L) and Pythium myriotylum on cocoyam (Xanthosoma sagittifolium L Schott). A rhamnolipid-deficient and a phenazine-deficient mutant of PNA1 were used either separately or jointly in plant experiments. When the mutants were applied separately, no disease-suppressive effect was observed, although both mutants still produced one of the antagonistic compounds (phenazines or rhamnolipids). When the mutants were concurrently introduced in the soil, the biocontrol activity was restored to wild-type levels. Bean seeds developed significantly less pre-emergence damping-off caused by P. splendens when treated with a mixture of purified phenazine-1-carboxamide and rhamnolipids than with any of the chemicals alone. When phenazines and rhamnolipids were combined at concentrations that had no observable effects when the metabolites were applied separately, mycelial growth of P. myriotylum was significantly reduced. In addition, microscopic analysis revealed substantial vacuolization and disintegration of Pythium hyphae after incubation in liquid medium amended with both metabolites. Results of this study indicate that phenazines and biosurfactants are acting synergistically in the control of Pythium spp.},
  author       = {Perneel, Maaike and D'hondt, Liesbet and De Maeyer, Katrien and Adiobo, Amayana and Rabaey, Korneel and H{\"o}fte, Monica},
  issn         = {1462-2912},
  journal      = {ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {PHENAZINE-1-CARBOXYLIC ACID,ANTIFUNGAL ACTIVITY,FLUORESCENT PSEUDOMONAS,IN-VITRO,PHYTOPHTHORA-CRYPTOGEA,CHLORORAPHIS PCL1391,PSEUDOMONAS-AERUGINOSA PNA1,SUGAR-BEET RHIZOSPHERE,QUINOLONE SIGNAL,WITLOOF CHICORY},
  language     = {eng},
  number       = {3},
  pages        = {778--788},
  title        = {Phenazines and biosurfactants interact in the biological control of soil-borne diseases caused by Pythium spp.},
  url          = {http://dx.doi.org/10.1111/j.1462-2920.2007.01501.x},
  volume       = {10},
  year         = {2008},
}

Chicago
Perneel, Maaike, Liesbet D’hondt, Katrien De Maeyer, Amayana Adiobo, Korneel Rabaey, and Monica Höfte. 2008. “Phenazines and Biosurfactants Interact in the Biological Control of Soil-borne Diseases Caused by Pythium Spp.” Environmental Microbiology 10 (3): 778–788.
APA
Perneel, M., D’hondt, L., De Maeyer, K., Adiobo, A., Rabaey, K., & Höfte, M. (2008). Phenazines and biosurfactants interact in the biological control of soil-borne diseases caused by Pythium spp. ENVIRONMENTAL MICROBIOLOGY, 10(3), 778–788.
Vancouver
1.
Perneel M, D’hondt L, De Maeyer K, Adiobo A, Rabaey K, Höfte M. Phenazines and biosurfactants interact in the biological control of soil-borne diseases caused by Pythium spp. ENVIRONMENTAL MICROBIOLOGY. 2008;10(3):778–88.
MLA
Perneel, Maaike, Liesbet D’hondt, Katrien De Maeyer, et al. “Phenazines and Biosurfactants Interact in the Biological Control of Soil-borne Diseases Caused by Pythium Spp.” ENVIRONMENTAL MICROBIOLOGY 10.3 (2008): 778–788. Print.