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Vaccine testing of a recombinant activation-associated secreted protein (ASP1) from Ostertagia ostertagi

Peter Geldhof UGent, Yves Meyvis UGent, Jozef Vercruysse UGent and Edwin Claerebout UGent (2008) PARASITE IMMUNOLOGY. 30(1). p.57-60
abstract
Previous vaccination trials against the economically important cattle parasite Ostertagia ostertagi have indicated the protective capacity of activation-associated secreted proteins (ASPs). The further development of these antigens into a commercial vaccine will require their recombinant expression. The aim of the current study was to clone and express Oo-asp1 in a baculovirus expression system and to evaluate the protective capacity of the recombinant protein against an O. ostertagi challenge infection in cattle. The full coding sequence of Oo-asp1 was cloned in a baculovirus expression vector in frame with a carboxy-terminal Histidine tag and recombinant virus was used to infect an insect cell culture. Western blot analysis with anti-His and anti-Oo-ASP1 antibodies showed the production of recombinant Oo-ASP1. The cell pellet containing the recombinant was subsequently used to immunize seven calves three times intramuscularly with QuilA as adjuvant. Control animals were solely injected with the QuilA adjuvant. The challenge infection with O. ostertagi consisted of 30 000 L3 larvae per animal given over 30 days (1000 larvae/day, 5 days/week) and started the same day as the final immunization. Immunization with the recombinant Oo-ASP1 did not result in any level of protection against the challenge infection. There was no reduction in faecal egg output or in worm burdens. Moreover, Western blot analyses and ELISA indicated that, although the animals raised an antibody response against the recombinant Oo-ASP1, there was hardly a response against the native Oo-ASP1, suggesting that the baculovirus expressed recombinant was wrongly folded or lacked essential secondary modifications. Further analysis of the structure of the native ASPs and their glycosylations is being done.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ANCYLOSTOMA-CANINUM, ANTIGENS, vaccination, ASP, recombinant, Ostertagia ostertagi, HOOKWORM BURDEN, REDUCTIONS, FRACTION
journal title
PARASITE IMMUNOLOGY
Parasite Immunol.
volume
30
issue
1
pages
57-60 pages
publisher
BLACKWELL PUBLISHING
Web of Science type
Article
Web of Science id
000251587900008
JCR category
PARASITOLOGY
JCR impact factor
2.523 (2008)
JCR rank
8/25 (2008)
JCR quartile
2 (2008)
ISSN
0141-9838
DOI
10.1111/j.1365-3024.2007.01001.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
390842
handle
http://hdl.handle.net/1854/LU-390842
date created
2008-03-04 13:28:00
date last changed
2009-04-30 15:30:08
@article{390842,
  abstract     = {Previous vaccination trials against the economically important cattle parasite Ostertagia ostertagi have indicated the protective capacity of activation-associated secreted proteins (ASPs). The further development of these antigens into a commercial vaccine will require their recombinant expression. The aim of the current study was to clone and express Oo-asp1 in a baculovirus expression system and to evaluate the protective capacity of the recombinant protein against an O. ostertagi challenge infection in cattle. The full coding sequence of Oo-asp1 was cloned in a baculovirus expression vector in frame with a carboxy-terminal Histidine tag and recombinant virus was used to infect an insect cell culture. Western blot analysis with anti-His and anti-Oo-ASP1 antibodies showed the production of recombinant Oo-ASP1. The cell pellet containing the recombinant was subsequently used to immunize seven calves three times intramuscularly with QuilA as adjuvant. Control animals were solely injected with the QuilA adjuvant. The challenge infection with O. ostertagi consisted of 30 000 L3 larvae per animal given over 30 days (1000 larvae/day, 5 days/week) and started the same day as the final immunization. Immunization with the recombinant Oo-ASP1 did not result in any level of protection against the challenge infection. There was no reduction in faecal egg output or in worm burdens. Moreover, Western blot analyses and ELISA indicated that, although the animals raised an antibody response against the recombinant Oo-ASP1, there was hardly a response against the native Oo-ASP1, suggesting that the baculovirus expressed recombinant was wrongly folded or lacked essential secondary modifications. Further analysis of the structure of the native ASPs and their glycosylations is being done.},
  author       = {Geldhof, Peter and Meyvis, Yves and Vercruysse, Jozef and Claerebout, Edwin},
  issn         = {0141-9838},
  journal      = {PARASITE IMMUNOLOGY},
  keyword      = {ANCYLOSTOMA-CANINUM,ANTIGENS,vaccination,ASP,recombinant,Ostertagia ostertagi,HOOKWORM BURDEN,REDUCTIONS,FRACTION},
  language     = {eng},
  number       = {1},
  pages        = {57--60},
  publisher    = {BLACKWELL PUBLISHING},
  title        = {Vaccine testing of a recombinant activation-associated secreted protein (ASP1) from Ostertagia ostertagi},
  url          = {http://dx.doi.org/10.1111/j.1365-3024.2007.01001.x},
  volume       = {30},
  year         = {2008},
}

Chicago
Geldhof, Peter, Yves Meyvis, Jozef Vercruysse, and Edwin Claerebout. 2008. “Vaccine Testing of a Recombinant Activation-associated Secreted Protein (ASP1) from Ostertagia Ostertagi.” Parasite Immunology 30 (1): 57–60.
APA
Geldhof, P., Meyvis, Y., Vercruysse, J., & Claerebout, E. (2008). Vaccine testing of a recombinant activation-associated secreted protein (ASP1) from Ostertagia ostertagi. PARASITE IMMUNOLOGY, 30(1), 57–60.
Vancouver
1.
Geldhof P, Meyvis Y, Vercruysse J, Claerebout E. Vaccine testing of a recombinant activation-associated secreted protein (ASP1) from Ostertagia ostertagi. PARASITE IMMUNOLOGY. BLACKWELL PUBLISHING; 2008;30(1):57–60.
MLA
Geldhof, Peter, Yves Meyvis, Jozef Vercruysse, et al. “Vaccine Testing of a Recombinant Activation-associated Secreted Protein (ASP1) from Ostertagia Ostertagi.” PARASITE IMMUNOLOGY 30.1 (2008): 57–60. Print.