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Identification and characterization of a novel specific secreted protein family for selected members of the subfamily Ostertagiinae (Nematoda)

Heidi Saverwyns, Aline Visser UGent, AJ Nisbet, Iris Peelaers UGent, Kris Gevaert UGent, Jozef Vercruysse UGent, Edwin Claerebout UGent and Peter Geldhof UGent (2008) PARASITOLOGY. 135(1). p.63-70
abstract
It has been shown that the bovine abomasal parasite, Ostertagia ostertagi, drastically modulates its microenvironment, causing epithelial cell damage, accumulation of inflammatory cells and pH changes in the stomach. The mechanisms used by the parasite to change the abomasal environment are largely unknown, but an important role has been attributed to excretory-secretory (ES) products from the parasite. In this study we have identified proteins representing a novel ES protein family, characterized by the SCP/Tpx-1/Ag5/PR-1/Sc7 protein motif. These proteins were named Oo-AL1 and Oo-AL2 (O. ostertagi ASP-like protein). Both proteins contain a signal peptide and 1 predicted N-glycosylation site. The transcript for Oo-AL1 was present from the L4 stage onwards in both male and female adult worms, whereas the Oo-AL2 transcript was hardly detectable. Western blots of somatic extracts and ES products from different developmental stages of O. ostertagi, probed with anti-Oo-AL1 antibodies, revealed Oo-AL proteins in the ES products of adult worms. An analysis of the nematode genome and EST databases indicated that these novel ES proteins are unique to O. ostertagi and its relative, Teladorsagia circumcincta, suggesting a key function in these abomasal parasites.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
VACCINATION, CIRCUMCINCTA, ANTIGENS, CLONING, HOOKWORM LARVAE, PARASITIC STAGES, ANCYLOSTOMA-CANINUM, PATHOGENESIS-RELATED PROTEINS, activation-associated secreted proteins (ASPs), Ostertagia ostertagi, excretory-secretory products, TRANSITION, EXPRESSION
journal title
PARASITOLOGY
Parasitology
volume
135
issue
1
pages
63-70 pages
publisher
Cambridge University Press
Web of Science type
Article
Web of Science id
000253151500008
JCR category
PARASITOLOGY
JCR impact factor
2.071 (2008)
JCR rank
10/25 (2008)
JCR quartile
2 (2008)
ISSN
0031-1820
DOI
10.1017/S0031182007003666
language
English
UGent publication?
yes
classification
A1
id
390837
handle
http://hdl.handle.net/1854/LU-390837
date created
2008-03-04 13:22:00
date last changed
2013-01-30 09:42:06
@article{390837,
  abstract     = {It has been shown that the bovine abomasal parasite, Ostertagia ostertagi, drastically modulates its microenvironment, causing epithelial cell damage, accumulation of inflammatory cells and pH changes in the stomach. The mechanisms used by the parasite to change the abomasal environment are largely unknown, but an important role has been attributed to excretory-secretory (ES) products from the parasite. In this study we have identified proteins representing a novel ES protein family, characterized by the SCP/Tpx-1/Ag5/PR-1/Sc7 protein motif. These proteins were named Oo-AL1 and Oo-AL2 (O. ostertagi ASP-like protein). Both proteins contain a signal peptide and 1 predicted N-glycosylation site. The transcript for Oo-AL1 was present from the L4 stage onwards in both male and female adult worms, whereas the Oo-AL2 transcript was hardly detectable. Western blots of somatic extracts and ES products from different developmental stages of O. ostertagi, probed with anti-Oo-AL1 antibodies, revealed Oo-AL proteins in the ES products of adult worms. An analysis of the nematode genome and EST databases indicated that these novel ES proteins are unique to O. ostertagi and its relative, Teladorsagia circumcincta, suggesting a key function in these abomasal parasites.},
  author       = {Saverwyns, Heidi and Visser, Aline and Nisbet, AJ and Peelaers, Iris and Gevaert, Kris and Vercruysse, Jozef and Claerebout, Edwin and Geldhof, Peter},
  issn         = {0031-1820},
  journal      = {PARASITOLOGY},
  keyword      = {VACCINATION,CIRCUMCINCTA,ANTIGENS,CLONING,HOOKWORM LARVAE,PARASITIC STAGES,ANCYLOSTOMA-CANINUM,PATHOGENESIS-RELATED PROTEINS,activation-associated secreted proteins (ASPs),Ostertagia ostertagi,excretory-secretory products,TRANSITION,EXPRESSION},
  language     = {eng},
  number       = {1},
  pages        = {63--70},
  publisher    = {Cambridge University Press},
  title        = {Identification and characterization of a novel specific secreted protein family for selected members of the subfamily Ostertagiinae (Nematoda)},
  url          = {http://dx.doi.org/10.1017/S0031182007003666},
  volume       = {135},
  year         = {2008},
}

Chicago
Saverwyns, Heidi, Aline Visser, AJ Nisbet, Iris Peelaers, Kris Gevaert, Jozef Vercruysse, Edwin Claerebout, and Peter Geldhof. 2008. “Identification and Characterization of a Novel Specific Secreted Protein Family for Selected Members of the Subfamily Ostertagiinae (Nematoda).” Parasitology 135 (1): 63–70.
APA
Saverwyns, H., Visser, A., Nisbet, A., Peelaers, I., Gevaert, K., Vercruysse, J., Claerebout, E., et al. (2008). Identification and characterization of a novel specific secreted protein family for selected members of the subfamily Ostertagiinae (Nematoda). PARASITOLOGY, 135(1), 63–70.
Vancouver
1.
Saverwyns H, Visser A, Nisbet A, Peelaers I, Gevaert K, Vercruysse J, et al. Identification and characterization of a novel specific secreted protein family for selected members of the subfamily Ostertagiinae (Nematoda). PARASITOLOGY. Cambridge University Press; 2008;135(1):63–70.
MLA
Saverwyns, Heidi, Aline Visser, AJ Nisbet, et al. “Identification and Characterization of a Novel Specific Secreted Protein Family for Selected Members of the Subfamily Ostertagiinae (Nematoda).” PARASITOLOGY 135.1 (2008): 63–70. Print.