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Relation between telomerase activity, hTERT and telomere length for intracranial tumours

(2007) ONCOLOGY REPORTS. 18(6). p.1571-1576
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Abstract
Human linear chromosomes are capped by specialized DNA-protein structures called telomeres. The present study analysed the telomerase activity, hTERT protein and telomere length in meningiomas and gliomas in relation to their WHO grading. Fifty-three freshly dissected tumour biopsies were analysed for telomerase activity, hTERT protein expression and telomere length. Telomerase activity was examined in 41 of the 53 biopsies. Telomerase activity was detected in 3 of 35 (8.6%) screened meningiomas (I benign, 1 atypical and I malignant meningioma). For hTERT expression, 56.4% of meningiomas were positive with a mean labelling index (hTERT LI) of 31.3% (SD=26.5) for the hTERT positive meningiomas. The mean telomere length for meningiomas was 6.983 kb (SD=1.969). For gliomas, no active telomerase was detected in 2 low-grade astrocytomas, whereas three of the four screened glioblastomas were positive for telomerase activity. The only hTERT protein positive astrocytoma had a mean labelling index of 9.0%. On the other hand, the hTERT LI for glioblastomas was 53.6% (SD=28.0). The two low-grade astrocytomas had a telomere length of 14.310 and 9.236 kb. The anaplastic astrocytoma had a telomere length of 4.903 kb and the glioblastomas 5.767 kb (SD=2.042). The normal meningeal and neuronal tissue is negative for telomerase activity and hTERT. The length was +/- 10.000 kb. These results indicate that telomere shortening may be a critical step in pathogenesis of atypical and malignant meningiomas and gliomas. Critical telomere shortening in vitro was shown to activate telomerase.
Keywords
telomere length, telomerase, intracranial tumours, REVERSE-TRANSCRIPTASE, IMMORTAL CELLS, BRAIN-TUMORS, IN-VITRO, RNA COMPONENT, PROGRESSION, CANCER, PROTEIN, EXPRESSION, MENINGIOMAS

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Chicago
Maes, Lode, Leander Van Neste, K Van Damme, Jean-Pierre Kalala Okito, Leo De Ridder, Sofie Bekaert, and Maria Cornelissen. 2007. “Relation Between Telomerase Activity, hTERT and Telomere Length for Intracranial Tumours.” Oncology Reports 18 (6): 1571–1576.
APA
Maes, Lode, Van Neste, L., Van Damme, K., Kalala Okito, J.-P., De Ridder, L., Bekaert, S., & Cornelissen, M. (2007). Relation between telomerase activity, hTERT and telomere length for intracranial tumours. ONCOLOGY REPORTS, 18(6), 1571–1576.
Vancouver
1.
Maes L, Van Neste L, Van Damme K, Kalala Okito J-P, De Ridder L, Bekaert S, et al. Relation between telomerase activity, hTERT and telomere length for intracranial tumours. ONCOLOGY REPORTS. 2007;18(6):1571–6.
MLA
Maes, Lode, Leander Van Neste, K Van Damme, et al. “Relation Between Telomerase Activity, hTERT and Telomere Length for Intracranial Tumours.” ONCOLOGY REPORTS 18.6 (2007): 1571–1576. Print.
@article{389652,
  abstract     = {Human linear chromosomes are capped by specialized DNA-protein structures called telomeres. The present study analysed the telomerase activity, hTERT protein and telomere length in meningiomas and gliomas in relation to their WHO grading. Fifty-three freshly dissected tumour biopsies were analysed for telomerase activity, hTERT protein expression and telomere length. Telomerase activity was examined in 41 of the 53 biopsies. Telomerase activity was detected in 3 of 35 (8.6\%) screened meningiomas (I benign, 1 atypical and I malignant meningioma). For hTERT expression, 56.4\% of meningiomas were positive with a mean labelling index (hTERT LI) of 31.3\% (SD=26.5) for the hTERT positive meningiomas. The mean telomere length for meningiomas was 6.983 kb (SD=1.969). For gliomas, no active telomerase was detected in 2 low-grade astrocytomas, whereas three of the four screened glioblastomas were positive for telomerase activity. The only hTERT protein positive astrocytoma had a mean labelling index of 9.0\%. On the other hand, the hTERT LI for glioblastomas was 53.6\% (SD=28.0). The two low-grade astrocytomas had a telomere length of 14.310 and 9.236 kb. The anaplastic astrocytoma had a telomere length of 4.903 kb and the glioblastomas 5.767 kb (SD=2.042). The normal meningeal and neuronal tissue is negative for telomerase activity and hTERT. The length was +/- 10.000 kb. These results indicate that telomere shortening may be a critical step in pathogenesis of atypical and malignant meningiomas and gliomas. Critical telomere shortening in vitro was shown to activate telomerase.},
  author       = {Maes, Lode and Van Neste, Leander and Van Damme, K and Kalala Okito, Jean-Pierre and De Ridder, Leo and Bekaert, Sofie and Cornelissen, Maria},
  issn         = {1021-335X},
  journal      = {ONCOLOGY REPORTS},
  keyword      = {telomere length,telomerase,intracranial tumours,REVERSE-TRANSCRIPTASE,IMMORTAL CELLS,BRAIN-TUMORS,IN-VITRO,RNA COMPONENT,PROGRESSION,CANCER,PROTEIN,EXPRESSION,MENINGIOMAS},
  language     = {eng},
  number       = {6},
  pages        = {1571--1576},
  title        = {Relation between telomerase activity, hTERT and telomere length for intracranial tumours},
  url          = {http://dx.doi.org/10.3892/or.18.6.1571},
  volume       = {18},
  year         = {2007},
}

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