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Relation between telomerase activity, hTERT and telomere length for intracranial tumours

Lode Maes UGent, Leander Van Neste UGent, K Van Damme, Jean-Pierre Kalala Okito UGent, Leo De Ridder, Sofie Bekaert UGent and Maria Cornelissen UGent (2007) ONCOLOGY REPORTS. 18(6). p.1571-1576
abstract
Human linear chromosomes are capped by specialized DNA-protein structures called telomeres. The present study analysed the telomerase activity, hTERT protein and telomere length in meningiomas and gliomas in relation to their WHO grading. Fifty-three freshly dissected tumour biopsies were analysed for telomerase activity, hTERT protein expression and telomere length. Telomerase activity was examined in 41 of the 53 biopsies. Telomerase activity was detected in 3 of 35 (8.6%) screened meningiomas (I benign, 1 atypical and I malignant meningioma). For hTERT expression, 56.4% of meningiomas were positive with a mean labelling index (hTERT LI) of 31.3% (SD=26.5) for the hTERT positive meningiomas. The mean telomere length for meningiomas was 6.983 kb (SD=1.969). For gliomas, no active telomerase was detected in 2 low-grade astrocytomas, whereas three of the four screened glioblastomas were positive for telomerase activity. The only hTERT protein positive astrocytoma had a mean labelling index of 9.0%. On the other hand, the hTERT LI for glioblastomas was 53.6% (SD=28.0). The two low-grade astrocytomas had a telomere length of 14.310 and 9.236 kb. The anaplastic astrocytoma had a telomere length of 4.903 kb and the glioblastomas 5.767 kb (SD=2.042). The normal meningeal and neuronal tissue is negative for telomerase activity and hTERT. The length was +/- 10.000 kb. These results indicate that telomere shortening may be a critical step in pathogenesis of atypical and malignant meningiomas and gliomas. Critical telomere shortening in vitro was shown to activate telomerase.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
telomere length, telomerase, intracranial tumours, REVERSE-TRANSCRIPTASE, IMMORTAL CELLS, BRAIN-TUMORS, IN-VITRO, RNA COMPONENT, PROGRESSION, CANCER, PROTEIN, EXPRESSION, MENINGIOMAS
journal title
ONCOLOGY REPORTS
Oncol. Rep.
volume
18
issue
6
pages
1571 - 1576
Web of Science type
Article
Web of Science id
000251353300031
JCR category
ONCOLOGY
JCR impact factor
1.597 (2007)
JCR rank
94/129 (2007)
JCR quartile
3 (2007)
ISSN
1021-335X
DOI
10.3892/or.18.6.1571
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
389652
handle
http://hdl.handle.net/1854/LU-389652
date created
2008-02-29 09:03:00
date last changed
2018-02-22 12:36:49
@article{389652,
  abstract     = {Human linear chromosomes are capped by specialized DNA-protein structures called telomeres. The present study analysed the telomerase activity, hTERT protein and telomere length in meningiomas and gliomas in relation to their WHO grading. Fifty-three freshly dissected tumour biopsies were analysed for telomerase activity, hTERT protein expression and telomere length. Telomerase activity was examined in 41 of the 53 biopsies. Telomerase activity was detected in 3 of 35 (8.6\%) screened meningiomas (I benign, 1 atypical and I malignant meningioma). For hTERT expression, 56.4\% of meningiomas were positive with a mean labelling index (hTERT LI) of 31.3\% (SD=26.5) for the hTERT positive meningiomas. The mean telomere length for meningiomas was 6.983 kb (SD=1.969). For gliomas, no active telomerase was detected in 2 low-grade astrocytomas, whereas three of the four screened glioblastomas were positive for telomerase activity. The only hTERT protein positive astrocytoma had a mean labelling index of 9.0\%. On the other hand, the hTERT LI for glioblastomas was 53.6\% (SD=28.0). The two low-grade astrocytomas had a telomere length of 14.310 and 9.236 kb. The anaplastic astrocytoma had a telomere length of 4.903 kb and the glioblastomas 5.767 kb (SD=2.042). The normal meningeal and neuronal tissue is negative for telomerase activity and hTERT. The length was +/- 10.000 kb. These results indicate that telomere shortening may be a critical step in pathogenesis of atypical and malignant meningiomas and gliomas. Critical telomere shortening in vitro was shown to activate telomerase.},
  author       = {Maes, Lode and Van Neste, Leander and Van Damme, K and Kalala Okito, Jean-Pierre and De Ridder, Leo and Bekaert, Sofie and Cornelissen, Maria},
  issn         = {1021-335X},
  journal      = {ONCOLOGY REPORTS},
  keyword      = {telomere length,telomerase,intracranial tumours,REVERSE-TRANSCRIPTASE,IMMORTAL CELLS,BRAIN-TUMORS,IN-VITRO,RNA COMPONENT,PROGRESSION,CANCER,PROTEIN,EXPRESSION,MENINGIOMAS},
  language     = {eng},
  number       = {6},
  pages        = {1571--1576},
  title        = {Relation between telomerase activity, hTERT and telomere length for intracranial tumours},
  url          = {http://dx.doi.org/10.3892/or.18.6.1571},
  volume       = {18},
  year         = {2007},
}

Chicago
Maes, Lode, Leander Van Neste, K Van Damme, Jean-Pierre Kalala Okito, Leo De Ridder, Sofie Bekaert, and Maria Cornelissen. 2007. “Relation Between Telomerase Activity, hTERT and Telomere Length for Intracranial Tumours.” Oncology Reports 18 (6): 1571–1576.
APA
Maes, Lode, Van Neste, L., Van Damme, K., Kalala Okito, J.-P., De Ridder, L., Bekaert, S., & Cornelissen, M. (2007). Relation between telomerase activity, hTERT and telomere length for intracranial tumours. ONCOLOGY REPORTS, 18(6), 1571–1576.
Vancouver
1.
Maes L, Van Neste L, Van Damme K, Kalala Okito J-P, De Ridder L, Bekaert S, et al. Relation between telomerase activity, hTERT and telomere length for intracranial tumours. ONCOLOGY REPORTS. 2007;18(6):1571–6.
MLA
Maes, Lode, Leander Van Neste, K Van Damme, et al. “Relation Between Telomerase Activity, hTERT and Telomere Length for Intracranial Tumours.” ONCOLOGY REPORTS 18.6 (2007): 1571–1576. Print.