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Comparison of different strategies to reduce acetate formation in Escherichia coli

Marjan De Mey (UGent) , Gaspard Lequeux (UGent) , Joeri Beauprez (UGent) , Jo Maertens (UGent) , Ellen Van Horen (UGent) , Wim Soetaert (UGent) , Peter A Vanrolleghem and Erick Vandamme (UGent)
(2007) BIOTECHNOLOGY PROGRESS. 23(5). p.1053-1063
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Abstract
E. coli cells produce acetate as an extracellular coproduct of aerobic cultures. Acetate is undesirable because it retards growth and inhibits protein formation. Most process designs or genetic modifications to minimize acetate formation aim at balancing growth rate and oxygen consumption. In this research, three genetic approaches to reduce acetate formation were investigated: (1) direct reduction of the carbon flow to acetate (ackA-pta, poxB knock-out); (2) anticipation on the underlying metabolic and regulatory mechanisms that lead to acetate (constitutive ppc expression mutant); and (3) both (1) and (2). Initially, these mutants were compared to the wild-type E. coli via batch cultures under aerobic conditions. Subsequently, these mutants were further characterized using metabolic flux analysis on continuous cultures. It is concluded that a combination of directly reducing the carbon flow to acetate and anticipating on the underlying metabolic and regulatory mechanism that lead to acetate, is the most promising approach to overcome acetate formation and improve recombinant protein production. These genetic modifications have no significant influence on the metabolism when growing the microorganisms under steady state at relatively low dilution rates (less than 0.4 h(-1)).
Keywords
MFA, Escherichia coli, acetate reduction

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Chicago
De Mey, Marjan, Gaspard Lequeux, Joeri Beauprez, Jo Maertens, Ellen Van Horen, Wim Soetaert, Peter A Vanrolleghem, and Erick Vandamme. 2007. “Comparison of Different Strategies to Reduce Acetate Formation in Escherichia Coli.” Biotechnology Progress 23 (5): 1053–1063.
APA
De Mey, Marjan, Lequeux, G., Beauprez, J., Maertens, J., Van Horen, E., Soetaert, W., Vanrolleghem, P. A., et al. (2007). Comparison of different strategies to reduce acetate formation in Escherichia coli. BIOTECHNOLOGY PROGRESS, 23(5), 1053–1063.
Vancouver
1.
De Mey M, Lequeux G, Beauprez J, Maertens J, Van Horen E, Soetaert W, et al. Comparison of different strategies to reduce acetate formation in Escherichia coli. BIOTECHNOLOGY PROGRESS. 2007;23(5):1053–63.
MLA
De Mey, Marjan, Gaspard Lequeux, Joeri Beauprez, et al. “Comparison of Different Strategies to Reduce Acetate Formation in Escherichia Coli.” BIOTECHNOLOGY PROGRESS 23.5 (2007): 1053–1063. Print.
@article{387813,
  abstract     = {E. coli cells produce acetate as an extracellular coproduct of aerobic cultures. Acetate is undesirable because it retards growth and inhibits protein formation. Most process designs or genetic modifications to minimize acetate formation aim at balancing growth rate and oxygen consumption. In this research, three genetic approaches to reduce acetate formation were investigated: (1) direct reduction of the carbon flow to acetate (ackA-pta, poxB knock-out); (2) anticipation on the underlying metabolic and regulatory mechanisms that lead to acetate (constitutive ppc expression mutant); and (3) both (1) and (2). Initially, these mutants were compared to the wild-type E. coli via batch cultures under aerobic conditions. Subsequently, these mutants were further characterized using metabolic flux analysis on continuous cultures. It is concluded that a combination of directly reducing the carbon flow to acetate and anticipating on the underlying metabolic and regulatory mechanism that lead to acetate, is the most promising approach to overcome acetate formation and improve recombinant protein production. These genetic modifications have no significant influence on the metabolism when growing the microorganisms under steady state at relatively low dilution rates (less than 0.4 h(-1)).},
  author       = {De Mey, Marjan and Lequeux, Gaspard and Beauprez, Joeri and Maertens, Jo and Van Horen, Ellen and Soetaert, Wim and Vanrolleghem, Peter A and Vandamme, Erick},
  issn         = {8756-7938},
  journal      = {BIOTECHNOLOGY PROGRESS},
  keyword      = {MFA,Escherichia coli,acetate reduction},
  language     = {eng},
  number       = {5},
  pages        = {1053--1063},
  title        = {Comparison of different strategies to reduce acetate formation in Escherichia coli},
  url          = {http://dx.doi.org/10.1021/bp070170g},
  volume       = {23},
  year         = {2007},
}

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