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Comparison of different strategies to reduce acetate formation in Escherichia coli

Marjan De Mey UGent, Gaspard Lequeux UGent, Joeri Beauprez, Jo Maertens UGent, Ellen Van Horen UGent, Wim Soetaert UGent, Peter A Vanrolleghem and Erick Vandamme (2007) BIOTECHNOLOGY PROGRESS. 23(5). p.1053-1063
abstract
E. coli cells produce acetate as an extracellular coproduct of aerobic cultures. Acetate is undesirable because it retards growth and inhibits protein formation. Most process designs or genetic modifications to minimize acetate formation aim at balancing growth rate and oxygen consumption. In this research, three genetic approaches to reduce acetate formation were investigated: (1) direct reduction of the carbon flow to acetate (ackA-pta, poxB knock-out); (2) anticipation on the underlying metabolic and regulatory mechanisms that lead to acetate (constitutive ppc expression mutant); and (3) both (1) and (2). Initially, these mutants were compared to the wild-type E. coli via batch cultures under aerobic conditions. Subsequently, these mutants were further characterized using metabolic flux analysis on continuous cultures. It is concluded that a combination of directly reducing the carbon flow to acetate and anticipating on the underlying metabolic and regulatory mechanism that lead to acetate, is the most promising approach to overcome acetate formation and improve recombinant protein production. These genetic modifications have no significant influence on the metabolism when growing the microorganisms under steady state at relatively low dilution rates (less than 0.4 h(-1)).
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
MFA, Escherichia coli, acetate reduction
journal title
BIOTECHNOLOGY PROGRESS
Biotechnol. Prog.
volume
23
issue
5
pages
1053-1063 pages
Web of Science type
Article
Web of Science id
000250010000009
JCR category
FOOD SCIENCE & TECHNOLOGY
JCR impact factor
2.224 (2007)
JCR rank
11/102 (2007)
JCR quartile
1 (2007)
ISSN
8756-7938
DOI
10.1021/bp070170g
language
English
UGent publication?
yes
classification
A1
id
387813
handle
http://hdl.handle.net/1854/LU-387813
date created
2008-02-14 15:45:00
date last changed
2016-12-19 15:43:46
@article{387813,
  abstract     = {E. coli cells produce acetate as an extracellular coproduct of aerobic cultures. Acetate is undesirable because it retards growth and inhibits protein formation. Most process designs or genetic modifications to minimize acetate formation aim at balancing growth rate and oxygen consumption. In this research, three genetic approaches to reduce acetate formation were investigated: (1) direct reduction of the carbon flow to acetate (ackA-pta, poxB knock-out); (2) anticipation on the underlying metabolic and regulatory mechanisms that lead to acetate (constitutive ppc expression mutant); and (3) both (1) and (2). Initially, these mutants were compared to the wild-type E. coli via batch cultures under aerobic conditions. Subsequently, these mutants were further characterized using metabolic flux analysis on continuous cultures. It is concluded that a combination of directly reducing the carbon flow to acetate and anticipating on the underlying metabolic and regulatory mechanism that lead to acetate, is the most promising approach to overcome acetate formation and improve recombinant protein production. These genetic modifications have no significant influence on the metabolism when growing the microorganisms under steady state at relatively low dilution rates (less than 0.4 h(-1)).},
  author       = {De Mey, Marjan and Lequeux, Gaspard and Beauprez, Joeri and Maertens, Jo and Van Horen, Ellen and Soetaert, Wim and Vanrolleghem, Peter A and Vandamme, Erick},
  issn         = {8756-7938},
  journal      = {BIOTECHNOLOGY PROGRESS},
  keyword      = {MFA,Escherichia coli,acetate reduction},
  language     = {eng},
  number       = {5},
  pages        = {1053--1063},
  title        = {Comparison of different strategies to reduce acetate formation in Escherichia coli},
  url          = {http://dx.doi.org/10.1021/bp070170g},
  volume       = {23},
  year         = {2007},
}

Chicago
De Mey, Marjan, Gaspard Lequeux, Joeri Beauprez, Jo Maertens, Ellen Van Horen, Wim Soetaert, Peter A Vanrolleghem, and Erick Vandamme. 2007. “Comparison of Different Strategies to Reduce Acetate Formation in Escherichia Coli.” Biotechnology Progress 23 (5): 1053–1063.
APA
De Mey, Marjan, Lequeux, G., Beauprez, J., Maertens, J., Van Horen, E., Soetaert, W., Vanrolleghem, P. A., et al. (2007). Comparison of different strategies to reduce acetate formation in Escherichia coli. BIOTECHNOLOGY PROGRESS, 23(5), 1053–1063.
Vancouver
1.
De Mey M, Lequeux G, Beauprez J, Maertens J, Van Horen E, Soetaert W, et al. Comparison of different strategies to reduce acetate formation in Escherichia coli. BIOTECHNOLOGY PROGRESS. 2007;23(5):1053–63.
MLA
De Mey, Marjan, Gaspard Lequeux, Joeri Beauprez, et al. “Comparison of Different Strategies to Reduce Acetate Formation in Escherichia Coli.” BIOTECHNOLOGY PROGRESS 23.5 (2007): 1053–1063. Print.