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Minimizing acetate formation in E. coli fermentations

Marjan De Mey (UGent) , Sofie De Maeseneire (UGent) , Wim Soetaert (UGent) and Erick Vandamme (UGent)
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Abstract
Escherichia coli remains the best established production organisms in industrial biotechnology. However, during aerobic fermentation runs at high growth rates, considerable amounts of acetate are accumulated as by-product. This by-product has negative effects on growth and protein production. Over the last 20 years, substantial research efforts have been spent to reduce acetate accumulation during aerobic growth of E. coli on glucose. From the onset it was clear that this quest should not be a simple nor uncomplicated one. Simple deletion of the acetate pathway, reduced the acetate accumulation, but instead other by-products were formed. This minireview gives a clear outline of these research efforts and the outcome of them, including bioprocess level approaches and genetic approaches. Recently, the latter seems to have some promising results.
Keywords
metabolic engineering, Escherichia coli, acetate reduction, HIGH-CELL-DENSITY, CENTRAL CARBON METABOLISM, SUCCINIC ACID PRODUCTION, FED-BATCH FERMENTATIONS, TUNING GENETIC-CONTROL, D-LACTATE PRODUCTION, ACETIC-ACID, PYRUVATE OXIDASE, FLUX ANALYSIS, ACKA-PTA

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Citation

Please use this url to cite or link to this publication:

MLA
De Mey, Marjan et al. “Minimizing Acetate Formation in E. Coli Fermentations.” JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 34.11 (2007): 689–700. Print.
APA
De Mey, Marjan, De Maeseneire, S., Soetaert, W., & Vandamme, E. (2007). Minimizing acetate formation in E. coli fermentations. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 34(11), 689–700.
Chicago author-date
De Mey, Marjan, Sofie De Maeseneire, Wim Soetaert, and Erick Vandamme. 2007. “Minimizing Acetate Formation in E. Coli Fermentations.” Journal of Industrial Microbiology & Biotechnology 34 (11): 689–700.
Chicago author-date (all authors)
De Mey, Marjan, Sofie De Maeseneire, Wim Soetaert, and Erick Vandamme. 2007. “Minimizing Acetate Formation in E. Coli Fermentations.” Journal of Industrial Microbiology & Biotechnology 34 (11): 689–700.
Vancouver
1.
De Mey M, De Maeseneire S, Soetaert W, Vandamme E. Minimizing acetate formation in E. coli fermentations. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY. 2007;34(11):689–700.
IEEE
[1]
M. De Mey, S. De Maeseneire, W. Soetaert, and E. Vandamme, “Minimizing acetate formation in E. coli fermentations,” JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, vol. 34, no. 11, pp. 689–700, 2007.
@article{387812,
  abstract     = {Escherichia coli remains the best established production organisms in industrial biotechnology. However, during aerobic fermentation runs at high growth rates, considerable amounts of acetate are accumulated as by-product. This by-product has negative effects on growth and protein production. Over the last 20 years, substantial research efforts have been spent to reduce acetate accumulation during aerobic growth of E. coli on glucose. From the onset it was clear that this quest should not be a simple nor uncomplicated one. Simple deletion of the acetate pathway, reduced the acetate accumulation, but instead other by-products were formed. This minireview gives a clear outline of these research efforts and the outcome of them, including bioprocess level approaches and genetic approaches. Recently, the latter seems to have some promising results.},
  author       = {De Mey, Marjan and De Maeseneire, Sofie and Soetaert, Wim and Vandamme, Erick},
  issn         = {1367-5435},
  journal      = {JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY},
  keywords     = {metabolic engineering,Escherichia coli,acetate reduction,HIGH-CELL-DENSITY,CENTRAL CARBON METABOLISM,SUCCINIC ACID PRODUCTION,FED-BATCH FERMENTATIONS,TUNING GENETIC-CONTROL,D-LACTATE PRODUCTION,ACETIC-ACID,PYRUVATE OXIDASE,FLUX ANALYSIS,ACKA-PTA},
  language     = {eng},
  number       = {11},
  pages        = {689--700},
  title        = {Minimizing acetate formation in E. coli fermentations},
  url          = {http://dx.doi.org/10.1007/s10295-007-0244-2},
  volume       = {34},
  year         = {2007},
}

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