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Lipoplex-loaded microbubbles for gene delivery: A Trojan horse controlled by ultrasound

Ine Lentacker (UGent) , Stefaan De Smedt (UGent) , Jo Demeester (UGent) , Veerle Van Marck (UGent) , Marc Bracke (UGent) and Niek Sanders (UGent)
(2007) ADVANCED FUNCTIONAL MATERIALS. 17(12). p.1910-1916
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Abstract
Cationic poly(ethylene glycol)ylated (PEGylated) liposomes are one of the most important gene transfer reagents in non-viral gene therapy. However, the low transfection efficiencies of highly PEGylated lipoplexes currently hamper their clinical use. Recently, ultrasound has been used in combination with microbubbles to enhance the uptake of genes in different cell types. However, the gene transfer efficiency still remains low in these experiments. To overcome the limitations of both techniques, we present the attachment of PEGylated lipoplexes to microbubbles via biotin-avidin-biotin linkages. Exposure of these lipoplex-loaded microbubbles to ultrasound results in the release of unaltered lipoplexes. Furthermore, these lipoplex-loaded microbubbles exhibit much higher transfection efficiencies than "free" PEGylated lipoplexes or naked plasmid DNA (pDNA) when combined with microbubbles and ultrasound. Interestingly, the lipoplex-loaded microbubbles only transfect cells when exposed to ultrasound, which is promising for space- and time-controlled gene transfer. Finally, this novel Trojan-horse-like concept can also be exploited to achieve the ultrasound- triggered release of nanoparticles containing other therapeutic agents such as anticancer drugs.

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MLA
Lentacker, Ine et al. “Lipoplex-loaded Microbubbles for Gene Delivery: A Trojan Horse Controlled by Ultrasound.” ADVANCED FUNCTIONAL MATERIALS 17.12 (2007): 1910–1916. Print.
APA
Lentacker, I., De Smedt, S., Demeester, J., Van Marck, V., Bracke, M., & Sanders, N. (2007). Lipoplex-loaded microbubbles for gene delivery: A Trojan horse controlled by ultrasound. ADVANCED FUNCTIONAL MATERIALS, 17(12), 1910–1916.
Chicago author-date
Lentacker, Ine, Stefaan De Smedt, Jo Demeester, Veerle Van Marck, Marc Bracke, and Niek Sanders. 2007. “Lipoplex-loaded Microbubbles for Gene Delivery: A Trojan Horse Controlled by Ultrasound.” Advanced Functional Materials 17 (12): 1910–1916.
Chicago author-date (all authors)
Lentacker, Ine, Stefaan De Smedt, Jo Demeester, Veerle Van Marck, Marc Bracke, and Niek Sanders. 2007. “Lipoplex-loaded Microbubbles for Gene Delivery: A Trojan Horse Controlled by Ultrasound.” Advanced Functional Materials 17 (12): 1910–1916.
Vancouver
1.
Lentacker I, De Smedt S, Demeester J, Van Marck V, Bracke M, Sanders N. Lipoplex-loaded microbubbles for gene delivery: A Trojan horse controlled by ultrasound. ADVANCED FUNCTIONAL MATERIALS. WILEY-V C H VERLAG GMBH; 2007;17(12):1910–6.
IEEE
[1]
I. Lentacker, S. De Smedt, J. Demeester, V. Van Marck, M. Bracke, and N. Sanders, “Lipoplex-loaded microbubbles for gene delivery: A Trojan horse controlled by ultrasound,” ADVANCED FUNCTIONAL MATERIALS, vol. 17, no. 12, pp. 1910–1916, 2007.
@article{384664,
  abstract     = {{Cationic poly(ethylene glycol)ylated (PEGylated) liposomes are one of the most important gene transfer reagents in non-viral gene therapy. However, the low transfection efficiencies of highly PEGylated lipoplexes currently hamper their clinical use. Recently, ultrasound has been used in combination with microbubbles to enhance the uptake of genes in different cell types. However, the gene transfer efficiency still remains low in these experiments. To overcome the limitations of both techniques, we present the attachment of PEGylated lipoplexes to microbubbles via biotin-avidin-biotin linkages. Exposure of these lipoplex-loaded microbubbles to ultrasound results in the release of unaltered lipoplexes. Furthermore, these lipoplex-loaded microbubbles exhibit much higher transfection efficiencies than "free" PEGylated lipoplexes or naked plasmid DNA (pDNA) when combined with microbubbles and ultrasound. Interestingly, the lipoplex-loaded microbubbles only transfect cells when exposed to ultrasound, which is promising for space- and time-controlled gene transfer. Finally, this novel Trojan-horse-like concept can also be exploited to achieve the ultrasound- triggered release of nanoparticles containing other therapeutic agents such as anticancer drugs.}},
  author       = {{Lentacker, Ine and De Smedt, Stefaan and Demeester, Jo and Van Marck, Veerle and Bracke, Marc and Sanders, Niek}},
  issn         = {{1616-301X}},
  journal      = {{ADVANCED FUNCTIONAL MATERIALS}},
  language     = {{eng}},
  number       = {{12}},
  pages        = {{1910--1916}},
  publisher    = {{WILEY-V C H VERLAG GMBH}},
  title        = {{Lipoplex-loaded microbubbles for gene delivery: A Trojan horse controlled by ultrasound}},
  url          = {{http://dx.doi.org/10.1002/adfm.200700106}},
  volume       = {{17}},
  year         = {{2007}},
}

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