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Lipoplex-loaded microbubbles for gene delivery: A Trojan horse controlled by ultrasound

Ine Lentacker UGent, Stefaan De Smedt UGent, Jo Demeester UGent, Veerle Van Marck UGent, Marc Bracke UGent and Niek Sanders UGent (2007) ADVANCED FUNCTIONAL MATERIALS. 17(12). p.1910-1916
abstract
Cationic poly(ethylene glycol)ylated (PEGylated) liposomes are one of the most important gene transfer reagents in non-viral gene therapy. However, the low transfection efficiencies of highly PEGylated lipoplexes currently hamper their clinical use. Recently, ultrasound has been used in combination with microbubbles to enhance the uptake of genes in different cell types. However, the gene transfer efficiency still remains low in these experiments. To overcome the limitations of both techniques, we present the attachment of PEGylated lipoplexes to microbubbles via biotin-avidin-biotin linkages. Exposure of these lipoplex-loaded microbubbles to ultrasound results in the release of unaltered lipoplexes. Furthermore, these lipoplex-loaded microbubbles exhibit much higher transfection efficiencies than "free" PEGylated lipoplexes or naked plasmid DNA (pDNA) when combined with microbubbles and ultrasound. Interestingly, the lipoplex-loaded microbubbles only transfect cells when exposed to ultrasound, which is promising for space- and time-controlled gene transfer. Finally, this novel Trojan-horse-like concept can also be exploited to achieve the ultrasound- triggered release of nanoparticles containing other therapeutic agents such as anticancer drugs.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
journal title
ADVANCED FUNCTIONAL MATERIALS
Adv. Funct. Mater.
volume
17
issue
12
pages
1910-1916 pages
publisher
WILEY-V C H VERLAG GMBH
Web of Science type
Article
Web of Science id
000248920000006
JCR category
PHYSICS, APPLIED
JCR impact factor
7.496 (2007)
JCR rank
3/88 (2007)
JCR quartile
1 (2007)
ISSN
1616-301X
DOI
10.1002/adfm.200700106
language
English
UGent publication?
yes
classification
A1
id
384664
handle
http://hdl.handle.net/1854/LU-384664
date created
2008-01-16 16:25:00
date last changed
2009-05-28 10:05:08
@article{384664,
  abstract     = {Cationic poly(ethylene glycol)ylated (PEGylated) liposomes are one of the most important gene transfer reagents in non-viral gene therapy. However, the low transfection efficiencies of highly PEGylated lipoplexes currently hamper their clinical use. Recently, ultrasound has been used in combination with microbubbles to enhance the uptake of genes in different cell types. However, the gene transfer efficiency still remains low in these experiments. To overcome the limitations of both techniques, we present the attachment of PEGylated lipoplexes to microbubbles via biotin-avidin-biotin linkages. Exposure of these lipoplex-loaded microbubbles to ultrasound results in the release of unaltered lipoplexes. Furthermore, these lipoplex-loaded microbubbles exhibit much higher transfection efficiencies than {\textacutedbl}free{\textacutedbl} PEGylated lipoplexes or naked plasmid DNA (pDNA) when combined with microbubbles and ultrasound. Interestingly, the lipoplex-loaded microbubbles only transfect cells when exposed to ultrasound, which is promising for space- and time-controlled gene transfer. Finally, this novel Trojan-horse-like concept can also be exploited to achieve the ultrasound- triggered release of nanoparticles containing other therapeutic agents such as anticancer drugs.},
  author       = {Lentacker, Ine and De Smedt, Stefaan and Demeester, Jo and Van Marck, Veerle and Bracke, Marc and Sanders, Niek},
  issn         = {1616-301X},
  journal      = {ADVANCED FUNCTIONAL MATERIALS},
  language     = {eng},
  number       = {12},
  pages        = {1910--1916},
  publisher    = {WILEY-V C H VERLAG GMBH},
  title        = {Lipoplex-loaded microbubbles for gene delivery: A Trojan horse controlled by ultrasound},
  url          = {http://dx.doi.org/10.1002/adfm.200700106},
  volume       = {17},
  year         = {2007},
}

Chicago
Lentacker, Ine, Stefaan De Smedt, Jo Demeester, Veerle Van Marck, Marc Bracke, and Niek Sanders. 2007. “Lipoplex-loaded Microbubbles for Gene Delivery: A Trojan Horse Controlled by Ultrasound.” Advanced Functional Materials 17 (12): 1910–1916.
APA
Lentacker, I., De Smedt, S., Demeester, J., Van Marck, V., Bracke, M., & Sanders, N. (2007). Lipoplex-loaded microbubbles for gene delivery: A Trojan horse controlled by ultrasound. ADVANCED FUNCTIONAL MATERIALS, 17(12), 1910–1916.
Vancouver
1.
Lentacker I, De Smedt S, Demeester J, Van Marck V, Bracke M, Sanders N. Lipoplex-loaded microbubbles for gene delivery: A Trojan horse controlled by ultrasound. ADVANCED FUNCTIONAL MATERIALS. WILEY-V C H VERLAG GMBH; 2007;17(12):1910–6.
MLA
Lentacker, Ine, Stefaan De Smedt, Jo Demeester, et al. “Lipoplex-loaded Microbubbles for Gene Delivery: A Trojan Horse Controlled by Ultrasound.” ADVANCED FUNCTIONAL MATERIALS 17.12 (2007): 1910–1916. Print.